Development of a selective enrichment broth supplemented with bacteriological charcoal and a high concentration of polymyxin B for the detection of Campylobacter jejuni and Campylobacter coli in chicken carcass rinses

Chon, Jung‐Whan; Kim, Hyun‐Sook; Yim, Jin-Hyeok; Park, Jun-Ho; Kim, Moo‐Sang; Seo, Kun‐Ho

doi:10.1016/j.ijfoodmicro.2013.01.018

Cited by 17 publications

(15 citation statements)

References 13 publications

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“…As alternative enrichment broth, PB has previously been described providing good selectivity against non-target flora in the enrichment procedure of Campylobacter ( Bolton and Robertson, 1982 ; Uyttendaele and Debevere, 1996 ; Jasson et al, 2009 ; Habib et al, 2011 ; Ugarte-Ruiz et al, 2012 ) with selective components polymyxin B, rifampicin, trimethoprim, and cycloheximide/amphotericin B. Polymyxin B is probably the component that inhibits the ESBL bacteria since it has been shown to be active against most Gram-negative bacteria ( Bolton and Robertson, 1982 ). Chon et al (2013b) used polymyxin B in enrichment broth with cefoperazone, and restored selectivity in that way. Some studies have, however, shown that PB may inhibit growth of some Campylobacter strains as well, resulting in false negative outcomes ( Baylis et al, 2000 ; Paulsen et al, 2005 ), especially for C. coli ( Goossens et al, 1986 ).…”

Section: Discussionmentioning

confidence: 99%

Quantification of Growth of Campylobacter and Extended Spectrum β-Lactamase Producing Bacteria Sheds Light on Black Box of Enrichment Procedures

2016

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Campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide, and is routinely found in meat originating from poultry, sheep, pigs, and cattle. Effective monitoring of Campylobacter contamination is dependent on the availability of reliable detection methods. The method of the International Organization for Standardization for the detection of Campylobacter spp. in food (ISO 10272-1:2006) recommends the use of Bolton broth (BB) as selective enrichment medium, including a pre-enrichment step of 4–6 h at 37°C to revive sublethally damaged cells prior to incubation for 2 days at 41.5°C. Recently the presence of abundantly growing extended spectrum β-lactamase producing Enterobacteriaceae (ESBL bacteria) has become one of the most important factors that interfere with the isolation of Campylobacter, resulting in false-negative detection. However, detailed growth dynamics of Campylobacter and its competitors remain unclear, where these would provide a solid base for further improvement of the enrichment procedure for Campylobacter. Other enrichment broths, such as Preston broth (PB) and BB plus clavulanic acid (BBc) have been suggested to inhibit competitive flora. Therefore, these different broths were used as enrichments to measure the growth kinetics of several strains of Campylobacter jejuni and ESBL bacteria separately, in co-culture and of strains in chicken samples. The maximum cell numbers and often the growth rates of Campylobacter in mixed culture with ESBL bacteria were significantly lower than in single cultures, indicating severe suppression of Campylobacter by ESBL bacteria, also in naturally contaminated samples. PB and BBc successfully diminished ESBL bacteria and might therefore be a better choice as enrichment medium in possibly ESBL-bacteria contaminated samples. The efficacy of a pre-enrichment step in the BB ISO-procedure was not supported for cold-stressed and non-stressed cells. Therefore, omission of this step (4–6 h at 37°C) might be advised to obtain a less troublesome protocol.

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Section: Discussionmentioning

confidence: 99%

Quantification of Growth of Campylobacter and Extended Spectrum β-Lactamase Producing Bacteria Sheds Light on Black Box of Enrichment Procedures

2016

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“…As reported in several studies, the recovery of Campylobacter may vary depending on the isolation protocol used. The use of enrichment broth not only limits the quantitative validity of the data, but may also affect strains which are found as dominant in case mixed populations are present (Jasson et al 2009;Habib et al 2011;Vidal et al 2012;Chon et al 2013;Hayashi et al 2013). Even without enrichment, different isolation protocols may have an effect on the strains that are being detected, which introduces further variation as to which populations are determined (Newell et al 2001;Potturi-Venkata et al 2007b;Williams et al 2012).…”

Section: Introductionmentioning

confidence: 99%

The effect of different isolation protocols on detection and molecular characterization of Campylobacter from poultry

Ugarte‐Ruiz

Wassenaar

Gómez-Barrero

et al. 2013

Lett Appl Microbiol

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Significance and Impact of Study: The tracing of Campylobacter through the food chain remains important to control campylobacteriosis in humans. Our study points out that the isolation method used affects the genotypes obtained, and this should be considered as a variant when comparing the results of surveillance studies. AbstractWe determined whether different methods to isolate Campylobacter (including the ISO standard 10272:2006-1) affected the genotypes detectable from poultry, at three points during slaughter: caecal content, neck skin and meat. Carcasses from 28 independent flocks were thus sampled (subset A). In addition, ten neck skin samples from four flocks, ten caecal samples from ten different flocks and ten unrelated meat samples obtained from local supermarkets were collected (subset B). Campylobacter was isolated using eight different protocols: with and without enrichment using Bolton broth, Preston broth or Campyfood broth (CFB), followed by culture on either modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) or Campyfood agar (CFA). All obtained isolates were genotyped for flaA-SVR, and over half of the isolates were also typed by MLST. The strain richness, as a measure of number of detected fla-genotypes, obtained from subset A neck skin and caecal samples was higher than that of meat samples. In half of the cases, within a flock, at least one identical fla-genotype was obtained at all three slaughter stages, suggestive of autologous contamination of carcasses. Enrichment reduced the observed richness of isolates, while CFA plates increased richness compared to mCCDA plates, irrespective of inclusion of an enrichment step. Because the isolation protocol used influences both the yield and the fla-genotype richness obtained from poultry, this variable should be taken into account when different studies are being compared.

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“…with the highest efficiency, an enrichment phase of the initial sample is a crucial step in culturing (Cocolin et al, 2002). Several methods have been developed to combine improved enrichment methods with other new methods such as realtime PCR or a commercially available immunochromatographic assay (Kawatsu et al, 2010;Chon et al, 2013;Suh et al, 2014). However, the total time requirements of these detection methods were 48 h or more.…”

Section: Discussionmentioning

confidence: 99%

“…Current research methods for the detection of Campylobacter suggest the need for an enrichment step to increase the target pathogen concentration and to revitalise stressed and injured cells of Salmonella, Listeria and Campylobacter (Moran et al, 2009;Lynch et al, 2011;Chon et al, 2013). The enriched pathogens then become more accurately identifiable by real-time PCR (Garrido et al, 2013).…”

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confidence: 99%

Rapid in-house detection method of Campylobacter spp. from food by redox potential monitoring combined with real-time PCR

Erdősi

Szakmár

Szili

et al. 2018

Acta Veterinaria Hungarica

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The rapid detection of Campylobacter spp. is of utmost importance for the reduction of infections in humans by contaminated food products. The standard culturing method (ISO 10272-1:2006) involves a high time and labour demand. In this paper, we present a method that reduces the detection time of Campylobacter spp. to or below one third as compared to the ISO method, at a reduced cost per test. We used redox potential change of enrichment cultures (Bolton broth with Bolton selective supplement) for reliably selecting Campylobacter-contaminated raw milk and broiler meat samples. Identification of Campylobacter spp. in the contaminated samples was done by real-time PCR method. Culturing time to conclusive redox monitoring varied between 6 and 24 h for positive samples, depending on the contamination rate, in contrast to 136 h with the standard culturing process. However, now the Campylobacter-negative majority of food samples will not need to be tested by real-time PCR because redox potential monitoring can identify them in the selective enrichment phase. This method could be potentially used as a faster alternative to the current standard ISO 10272-1:2006, for nonregulatory monitoring purposes.

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Development of a selective enrichment broth supplemented with bacteriological charcoal and a high concentration of polymyxin B for the detection of Campylobacter jejuni and Campylobacter coli in chicken carcass rinses

Cited by 17 publications

References 13 publications

Quantification of Growth of Campylobacter and Extended Spectrum β-Lactamase Producing Bacteria Sheds Light on Black Box of Enrichment Procedures

Quantification of Growth of Campylobacter and Extended Spectrum β-Lactamase Producing Bacteria Sheds Light on Black Box of Enrichment Procedures

The effect of different isolation protocols on detection and molecular characterization of Campylobacter from poultry

Rapid in-house detection method of Campylobacter spp. from food by redox potential monitoring combined with real-time PCR

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