Importance of Transcript Levels of Caspase-2 Isoforms S and L for Breast Carcinoma Progression

Abstract

Our results suggest that the role of caspase-2 isoforms in the progression of breast cancer may considerably differ between pre- and post-chemotherapy patients.

MiR-301a-3p Suppresses Estrogen Signaling by Directly Inhibiting ESR1 in ERα Positive Breast Cancer

“…Processing of the tissue samples was described in detail previously [29]. Histological classification of carcinomas was performed according to standard diagnostic procedures [30].…”

Dihydrotanshinone I induced apoptosis and autophagy through caspase dependent pathway in colon cancer

“…The inclusion of exon 9 leads to an in-frame stop codon in caspase-2 short isoform (casp-2S) mRNA, thus producing a truncated protein that inhibits cell death. Whereas the exclusion of exon 9 results in caspase-2 long isoform (casp-2 L) mRNA, which produces protein product inducing cell death (Brynychova et al, 2013;Han et al, 2013;Iwanaga et al, 2005;Puccini et al, 2013). Caspases cleave a number of different substrates in the cytoplasm or nucleus, leading to many morphologic features of apoptotic cell death.…”

Transcript expression and genetic variability analysis of caspases in breast carcinomas suggests CASP9 as the most interesting target

“…Blood samples from 615 patients for the genotyping validation study were collected from patients treated in The Faculty Hospital Motol, Institute for the Care for Mother and Child, The Fac ulty Hospital Kralovske Vinohrady (Prague, Czech Republic), and The Hospital Atlas (Zlin, Czech Republic). The processing of the tissue and blood samples was described in detail previously [10,18]. Histologi cal classification of carcinomas was performed according to standard diagnostic procedures [19].…”

“…We have recently found a lack of association of expression of (proapoptotic) isoform of caspase2, CASP2L, and its alternative (antiapoptotic) isoform CASP2S with the response to neoadjuvant chemotherapy in breast carcinoma patients [10]. Alternative caspase variants with prosurvival function have also been char acterized in caspase3, caspase8, and caspase9 [11][12][13][14][15].…”

“…Therefore, genetic alterations in regulatory regions of caspase 9 were assessed to gain functional insight into their importance for expression and alternative splicing in breast carcinoma samples. Due to the overexpression of CASP2L in tumors of breast carci noma patients demonstrated in our previous work [10], we also analyzed regulatory regions of the CASP2 gene.…”