Transcript expression and genetic variability analysis of caspases in breast carcinomas suggests CASP9 as the most interesting target

Brynychová, Veronika; Hlaváč, Viktor; Ehrlichová, Marie; Václavíková, Radka; Němcová‐Fürstová, Vlasta; Pecha, Václav; Trnková, Markéta; Mrhalová, Marcela; Kodet, Roman; Vrána, David; Gatěk, Jiří; Bendová, Marie; Vernerová, Zdeňka; Kovář, Jan; Souček, Pavel

doi:10.1515/cclm-2016-0271

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…27 In this study, the expression of CASP9 showed a significant decrease, although OXR1 was suppressed in ESCC. The decreased mRNA levels of CASP9 were found in different cancer cells, such as hepatocellular carcinoma, breast carcinomas, and colorectal cancer, [28][29][30] whereas the up-regulation of CASP9 was observed when the proliferation of tumors was inhibited. Some studies have reported that CASP9 can be down-regulated by multiple miRNAs in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-373 promotes the development of esophageal squamous cell carcinoma by targeting LATS2 and OXR1

Wang

Chang

et al. 2019

Int J Biol Markers

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Introduction: MicroRNA373 was highly expressed in many tumors including esophageal cancer. However, its molecular mechanism is still unclear, especially epigenetic modification, in esophageal squamous cell carcinoma (ESCC). Methods: In this study, we investigated serum levels of the miR-371-373 cluster in ESCC patients before and after surgical removal, and further focused on the expression level of miR-373-3p in tumor tissues of ESCC patients and its target genes. In addition, the epigenetic alterations of miR-373-3p promoter was analyzed. Results: The expression levels of miR-371-5p and miR-373-3p were significantly increased in preoperative serum of ESCC patients compared with that of healthy volunteers ( P<0.01); however, they dropped significantly after surgical removal ( P<0.01). Compared with adjacent normal tissues, miR-373-3p also showed significant up-regulation in cancer tissues ( P<0.05). The methylation levels of miR-373-3p promoter were 42.86% in ESCC cancer tissue and 66.67% in adjacent normal tissues. The low methylation of the miR-373-3p promoter may promote the expression of miR-373-3p. Large tumor suppressor 2 ( LATS2) and oxidation resistance 1( OXR1) are predicted to be targets of miR-373-3p by the bioinformatics method. They are the genes in the Hippo and the p53 signaling pathway, respectively. Their respective upstream genes, neurofibromatosis type 2 (NF2) and Jun Kinase, and the downstream genes, transcriptional co-activator with PDZ-binding motif (TAZ) and caspase 9, were also detected. The expression of all these genes were significantly decreased in ESCC cancer tissues compared with adjacent normal tissues. Conclusions: This study shows that DNA epigenetic modification in the miR-373-3p promoter region and the Hippo and p53 signaling pathways play important roles during the miR-373-3p mediating ESCC development process.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-373 promotes the development of esophageal squamous cell carcinoma by targeting LATS2 and OXR1

Wang

Chang

et al. 2019

Int J Biol Markers

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“…Caspases (cysteine-dependent aspartyl-specific protease) belong to a family of cysteine proteases that cleave target proteins at specific aspartate residues (Alnemri et al, 1996;Nicholson et al, 1995;Julien et al, 2016) and mediate proteolytic events indispensable for biological phenomena such as inflammation cell death or apoptosis (Sadowski-Debbing et al, 2002;Brynychova et al, 2016;Larsen & Sorensen 2017). It is now clear that caspases are thought to play a pivotal role in apoptosis as an evolutionarily conserved function (Seidelin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Sun¹,

Yan²,

Wang³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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mRNA expression levels in various tissues by using real-time PCR method. RT-qPCR analyses showed that turtles PsCaspase-14 mRNA was ubiquitously expressed in brain, liver, heart, lung, stomach, spleen, muscle, pancreas, gallbladder, kidney, and intestine tissues of control turtles, and RT-qPCR analyses further indicated that PsCaspase-14 mRNA was affected significantly in tissues of the turtles challenged by bacteria A. hydrophila and V.parahaemolyticus, suggesting that PsCaspase-14 might be involved in turtle antiviral immunity.

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Transcript expression and genetic variability analysis of caspases in breast carcinomas suggests CASP9 as the most interesting target

Abstract: Genetic variability in CASP9 and expression of its splicing variants present targets for further study.

Cited by 2 publications

References 36 publications

MicroRNA-373 promotes the development of esophageal squamous cell carcinoma by targeting LATS2 and OXR1

MicroRNA-373 promotes the development of esophageal squamous cell carcinoma by targeting LATS2 and OXR1

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