Immunohistochemical Characterization of Type II Pneumocyte Proliferation after Challenge with Type I Porcine Reproductive and Respiratory Syndrome Virus

Balka, Gyula; Ladinig, Andrea; Ritzmann, M.; Saalmüller, Armin; Gerner, Wilhelm; Käser, Tobias; Jakab, Csaba; Rusvai, Miklós; Weißenböck, H.

doi:10.1016/j.jcpa.2012.12.006

Cited by 10 publications

(21 citation statements)

References 28 publications

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“…Lesions were hypertrophy and hyperplasia of pneumocytes, septal infiltration by mononuclear cells, perivascular and intraalveolar accumulation of inflammatory cells and necrotic debris. Depending on their occurrence in different lung lobes and on the severity of the lesions, a total score per lung was calculated, applying a PRRSV specific scoring system that has been described elsewhere [ 27 ]. The mean values for the Austrian isolates were 52.4 ± 20.9 for AUT14-440 and 53.6 ± 9.4 for AUT13-883 (Figure 5 A).…”

Section: Resultsmentioning

confidence: 99%

Characterization of two Austrian porcine reproductive and respiratory syndrome virus (PRRSV) field isolates reveals relationship to East Asian strains

Sinn

Zieglowski

Koinig

et al. 2016

Vet Res

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Porcine reproductive and respiratory syndrome virus (PRRSV) causes major problems for the swine industry worldwide. Due to Austria’s central location in Europe, a large number of animals are transported through the country. However, little is known about current PRRSV strains and epidemiology. We determined full-length genome sequences of two Austrian field isolates (AUT13-883 and AUT14-440) from recent PRRSV outbreaks and of a related German isolate (GER09-613). Phylogenetic analysis revealed that the strains belong to European genotype 1 subtype 1 and form a cluster together with a South Korean strain. Remarkably, AUT14-440 infected the simian cell line MARC-145 without prior adaptation. In addition, this isolate showed exceptional deletions in nonstructural protein 2, in the overlapping region of glycoprotein 3 and 4 and in the 3′ untranslated region. Both Austrian isolates caused similar lung lesions but only pigs infected with AUT14-440 developed clear clinical signs of infection. Taken together, the genetic and biological characterization of two novel Austrian PRRSV field isolates revealed similarities to East Asian strains. This stresses the necessity for a more detailed analysis of current PRRSV strains in Europe beyond the determination of short ORF5 and ORF7 sequences.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-015-0293-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Characterization of two Austrian porcine reproductive and respiratory syndrome virus (PRRSV) field isolates reveals relationship to East Asian strains

Sinn

Zieglowski

Koinig

et al. 2016

Vet Res

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“…To assess typical PRRSV-induced lung lesions, an early necropsy time point two weeks post-challenge was chosen. PRRSV-specific lung lesions are likely to decrease significantly after the acute phase of infection [35]. In the acute phase, PRRSV infection in growing pigs mainly affects the lung, especially the alveolar macrophages, which are primary target cells of PRRSV [36].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of a Modified Live Virus Vaccine against Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Administered to 1-Day-Old Piglets in Front of Heterologous PRRSV-1 Challenge

et al. 2021

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PRRSV is one of the most important viruses in the global swine industry and is often controlled by the use of modified live virus (MLV) vaccines. This study assessed the impact of a PRRSV-1 MLV vaccine applied to 1-day-old piglets challenged on day 28 of life with a PRRSV-1 field isolate (AUT15-33). Twenty-one piglets were vaccinated within 24 h of birth (T02), whereas 20 piglets were left unvaccinated (T01). Necropsy was performed two weeks post-challenge. Comparing the two groups, T02 piglets showed significantly higher (p = 0.017) average daily weight gain. In addition, significantly lower (p < 0.0001) PRRSV RNA loads were measured in serum of T02 piglets at all investigated time points. All T01 piglets were viremic and shed virus in nasal swabs, whereas only 71.4 % and 38.1 % of the T02 group were viremic or shed virus, respectively. Piglets from T02 had significantly higher numbers (p < 0.0001) of IFN-γ producing lymphocytes compared to T01. At necropsy, differences in gross and histologic lung lesions were statistically significant (p = 0.012 and p < 0.0001, respectively) between the two groups. Hence, this MLV vaccine administered to 1-day-old piglets was able to protect piglets against PRRSV infection at weaning.

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“…The interstitial fibrosis was in an active early stage with prominent proliferative smooth muscle and epithelial changes as well as accumulation of alveolar macrophages [4]. The alveolar macrophages showed variable degrees of foamy cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

“…The thickened alveolar septa contained foci of elongated smooth-muscle-like cells that stained positively for alpha smooth muscle actin. The proliferating type II pneumocytes stained positively for TTF-1 and pan CK [4]. The alveolar macrophages stained positively for CD68.…”

Section: Case Reportmentioning

confidence: 99%

Procainamide-induced pulmonary fibrosis after orthotopic heart transplantation: a case report and literature review

Thangam

Nathan

Petrovica

et al. 2015

Cardiovascular Pathology

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Immunohistochemical Characterization of Type II Pneumocyte Proliferation after Challenge with Type I Porcine Reproductive and Respiratory Syndrome Virus

Cited by 10 publications

References 28 publications

Characterization of two Austrian porcine reproductive and respiratory syndrome virus (PRRSV) field isolates reveals relationship to East Asian strains

Characterization of two Austrian porcine reproductive and respiratory syndrome virus (PRRSV) field isolates reveals relationship to East Asian strains

Efficacy of a Modified Live Virus Vaccine against Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Administered to 1-Day-Old Piglets in Front of Heterologous PRRSV-1 Challenge

Procainamide-induced pulmonary fibrosis after orthotopic heart transplantation: a case report and literature review

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