Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma

Jia, Lingfei; Wei, Su-bi; Gong, Kai; Gan, Ye‐Hua; Yu, Guang‐Yan

doi:10.1371/journal.pone.0056634

Cited by 51 publications

(53 citation statements)

References 45 publications

(54 reference statements)

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“…Cell apoptosis was measured by TUNEL/DAPI double staining assay as the manual recommended. The ACHN cells were seeded in coverslips in 6-well plates with the same cell number per well (1x10 5 ). Cells harvested 48 h after transfection were washed with PBS/1% BSA and fixed in paraformaldehyde solution (4% in PBS) for 20 min at room temperature.…”

Section: Rna Isolation and Quantitative Real-time Polymerase Chain Rementioning

confidence: 99%

MicroRNA-195 targets VEGFR2 and has a tumor suppressive role in ACHN cells via PI3K/Akt and Raf/MEK/ERK signaling pathways

Sun

Wang

et al. 2016

International Journal of Oncology

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Abstract. Increasing evidence indicates that dysregulation of miR-195 may contribute to the occurrence and development of multiple types of human malignancies. However, the function and the mechanism of miR-195 in clear cell renal cell carcinoma (ccRCC) are still not fully understood. In the present study, we used qRT-PCR to detect the expression of miR-195 in ccRCC tissues and normal kidney tissues. MTT assay was performed to detect the cell viability of miR-195. Migration and invasion were evaluated by Transwell migration and Matrigel invasion assays, respectively. Additionally, apoptosis levels were evaluated using TUNEL assays, and signaling pathway changes were determined by western blot analysis. We observed that miR-195 was downregulated in clear cell renal cell carcinoma samples compared with normal renal samples. We identified that overexpression of miR-195 inhibited ACHN cell viability, migration, invasion, and it also induced cell apoptosis by targeting VEGFR2 via PI3K/Akt and Raf/MEK/ERK signaling pathways. These findings indicate that miR-195 has a tumor suppressive role in ACHN cells and miR-195 may be a promising candidate target for prevention and treatment of renal cell carcinoma.

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Section: Rna Isolation and Quantitative Real-time Polymerase Chain Rementioning

confidence: 99%

MicroRNA-195 targets VEGFR2 and has a tumor suppressive role in ACHN cells via PI3K/Akt and Raf/MEK/ERK signaling pathways

Sun

Wang

et al. 2016

International Journal of Oncology

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“…However, the human transcriptome comprises not only large numbers of protein‐coding messenger RNAs (mRNAs) but also a large set of noncoding RNAs (ncRNAs) that have structural, regulatory or unknown functions . In TSCC many microRNAs (miRNAs), such as miR‐195/184/138/21, have been shown to be dysregulated and are believed to contribute to its development and progression . In contrast, long noncoding RNAs (lncRNAs), tentatively defined as ncRNAs more than 200 nt in length, are also known to play key roles in cancer development, and aberrant expression of lncRNAs has been functionally associated with lung, breast and prostate carcinomas .…”

mentioning

confidence: 99%

Expression, regulation and roles of miR‐26a and MEG3 in tongue squamous cell carcinoma

Jia

Wei

Gan

et al. 2014

Intl Journal of Cancer

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191

195

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MicroRNA miR-26a and long noncoding RNA (lncRNA) MEG3 gene have been independently reported to be tumor suppressor genes in various cancers, but neither has been previously associated with tongue squamous cell carcinoma (TSCC). We report here that miR-26a and lncRNA MEG3 gene expression were both strongly reduced in TSCC compared with levels in matched nonmalignant tissues, and combined low expression levels of both miR-26a and MEG3 emerged as an independent prognostic factor for poor clinical outcome in TSCC patients. Assays in the human TSCC cell lines SCC-15 and CAL27 showed that miR26a targets the DNA methyltransferase 3B transcript and that its inhibition may result in the upregulation of MEG3, providing a plausible link between the observed reduction of miR-26a and MEG3 in TSCC tissue. Furthermore, the overexpression of miR-26a or MEG3 in SCC-15 and CAL27 cells inhibited cell proliferation and cell cycle progression, and promoted cell apoptosis. Considering the poor prognostic outcomes associated with reduced miR-26a and MEG3, our findings imply that these factors likely play important antitumor effects in TSCC pathogenesis. Furthermore, they represent potential prognostic biomarkers for stratification of TSCC patients.Tongue squamous cell carcinoma (TSCC) is the most common type of oral cancer and is well known for its high rate of proliferation and lymph nodal metastasis. 1 In clinical practice, the prediction of prognosis and planning of treatment for patients suffering from oral cancers are predominantly based on the tumor node metastasis (TNM) staging system. 2 However, in many cases of TSCC, these conventional clinical prognostic factors remain inadequate, and are unable to discriminate tumors of the same clinical stage that may have distinct clinical outcomes and different responses to the same treatments. Therefore, the identification and application of additional biomarkers into the prognostic system that better reflect the biologic diversity of TSCC and consequently predict clinical outcomes more accurately is desirable. 3 Differentially expressed genes identified from hundreds of cancer profiling studies over the last several years have yielded numerous biomarkers that have improved the subtyping, classification and diagnosis of tumors for both research and the clinic. However, the human transcriptome comprises not only large numbers of protein-coding messenger RNAs (mRNAs) but also a large set of noncoding RNAs (ncRNAs) that have structural, regulatory or unknown functions. 4 In TSCC many microRNAs (miRNAs), such as miR-195/184/ 138/21, have been shown to be dysregulated and are believed to contribute to its development and progression. [5][6][7][8] In contrast, long noncoding RNAs (lncRNAs), tentatively defined as ncRNAs more than 200 nt in length, 9 are also known to play key roles in cancer development, 10,11 and aberrant expression of lncRNAs has been functionally associated with lung, breast and prostate carcinomas. 12-14 However, whether lncRNA has relevance to TSCC biology had not been p...

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“…Various cancer-associated genes, such as miR-126, mR-195 and let-7c were reported to be associated with kinds of cancers [20][21][22]. For the up-regulated miR-21, its potential target genes include the tumor suppressors TIMP3 and PDCD4.…”

Section: Discussionmentioning

confidence: 99%

Up-Regulation of miR-21 Expression Predicate Advanced Clinicopathological Features and Poor Prognosis in Patients with Non-Small Cell Lung Cancer

Tian

Shan

Zhang

et al. 2015

Pathol. Oncol. Res.

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MicroRNAs (miRNAs) are endogenous small (19-24 nt long) noncoding RNAs that regulate gene expression in a sequence specific manner. An increasing association between miRNA and cancer has been recently reported. Lung cancer is globally responsible for 1.4 million deaths annually and is the leading cause of cancer-related deaths in both women and men. In this study, we investigated the miR-21 expression in non-small cell lung cancer (NSCLC) to evaluate their value in prognosis of this tumor. Here, we assess miR-21 expression in NSCLC and its clinical significance including survival analysis. The expression of miR-21 in matched normal and tumor tissues of NSCLC was evaluated using a quantitative real-time RT-PCR. A Kaplan-Meier survival curve was generated following a logrank test. It was observed that miR-21 expression was up-regulated in NSCLC tissues compared with noncancerous lung tissues (mean ± SD: 6.7 ± 2.3 vs. 3.7 ± 1.5, P < 0.001). The up-regulation of miR-21 in NSCLC cancer tissues was also significantly correlated with aggressive clinicopathological features. We found that the patients with high miR-21 expression have a higher tumor grade (P = 0.027) and are in higher risk of lymph node metastasis (P = 0.021). Moreover, the results of Kaplan-Meier analyses showed that NSCLC patients with the high miR-21 expression tend to have shorter overall survival and progression free survival (P < 0.001). The multivariate analysis clearly indicated that the high miR-21 expression in biopsy samples may be considered as an independent prognostic factor in NSCLC for decreased survival (RR 3.88; 95%CI, 2.47-6.11). Our data indicate the potential of miR-21 as a novel prognostic biomarker for NSCLC. Large well-designed studies with diverse populations and functional evaluations are warranted to confirm and extend our findings.

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Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma

Cited by 51 publications

References 45 publications

MicroRNA-195 targets VEGFR2 and has a tumor suppressive role in ACHN cells via PI3K/Akt and Raf/MEK/ERK signaling pathways

MicroRNA-195 targets VEGFR2 and has a tumor suppressive role in ACHN cells via PI3K/Akt and Raf/MEK/ERK signaling pathways

Expression, regulation and roles of miR‐26a and MEG3 in tongue squamous cell carcinoma

Up-Regulation of miR-21 Expression Predicate Advanced Clinicopathological Features and Poor Prognosis in Patients with Non-Small Cell Lung Cancer

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