MicroRNA-195 targets VEGFR2 and has a tumor suppressive role in ACHN cells via PI3K/Akt and Raf/MEK/ERK signaling pathways

Sun, Peng; Wang, Lu; Lu, Yun-Han; Liu, Yu-wei; Li, Lechen; Li, Yin; Zhang, Cheng; Zhao, Weiming; Shen, Baogen; Xu, Wanhai

doi:10.3892/ijo.2016.3608

Cited by 32 publications

(22 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current study identified that overexpression of miRNA-195 represses the expression level of VEGFR2 in AMC-HN-8 cells. VEGFR2 mediates a variety of important molecular signaling pathways via its downstream signal proteins, including Raf/MEK/ERK1/2, SRC/FAK and apoptosis-related proteins, which serve a key role in regulating tumor development (36). Data from the present study further support the hypothesis that inhibition of VEGFR2/Raf/MEK/ERK and VEGFR2/SRC/FAK pathways regulates cancer cell growth and migration, respectively, mediated by miRNA-195 upregulation in vitro.…”

Section: Discussionsupporting

confidence: 81%

MicroRNA‑195 is associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinoma

Pang

Dai

et al. 2018

Mol Med Report

View full text Add to dashboard Cite

MicroRNAs (miRNAs) have been reported to be associated with the modulation of tumor development, including alterations associated with the development of human laryngeal squamous cell carcinoma (LSCC). The present study was designed to investigate whether miRNA‑195 was associated with the pathophysiologic process of human LSCC and to identify its potential roles and underlying molecular mechanisms. To determine whether miRNA‑195 serves a role in LSCC, reverse transcription‑quantitative polymerase chain reaction was used to detect miRNA‑195 expression in LSCC tissues. The tumor‑suppressive effect of miRNA‑195 was determined by in vitro assays. Gain‑of‑function studies using miRNA‑195 mimics were performed to investigate cell viability, migration and invasion, and apoptosis in the AMC‑HN‑8 cell line. Western blotting was performed to reveal the molecular mechanisms of miRNA‑195 and its downstream signaling pathways in the LSCC AMC‑HN‑8 cell line. The present study demonstrated that miRNA‑195 is downregulated in primary LSCC tumors. Upregulating miRNA‑195 in vitro suppressed cell viability, migration and invasion in AMC‑HN‑8 cells. Overexpression of miRNA‑195 alone in AMC‑HN‑8 cells was sufficient to induce cell apoptosis, as identified by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Compared with the high expression of miRNA‑195 in AMC‑HN‑8 cells, the expression levels of vascular endothelial growth factor receptor‑II protein and downstream signaling pathway proteins, which were associated with cell viability, migration, invasion and apoptosis, were markedly decreased compared with control or miRNA‑195 negative control treatment group. Together, these data suggest the therapeutic potential of miRNA‑195 in modulating cell growth, migration and apoptosis during the pathophysiological progression of LSCC and that miRNA‑195 may serve as a potential therapeutic target in human LSCC.

show abstract

Section: Discussionsupporting

confidence: 81%

MicroRNA‑195 is associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinoma

Pang

Dai

et al. 2018

Mol Med Report

View full text Add to dashboard Cite

show abstract

“…To a further extent, PI3K/AKT/mTOR is identified as a highly enriched pathway in translocation RCC with TFE3 fusion (TFE3-tRCC) by miRNA microarray analysis [258]. Besides that PIK3R1 regulates EMT and stem-like phenotype of RCC cells through the AKT/GSK3β/CTNNB1 pathway [259], FoxO, PKCε, TPD52, NOTCH1, ETS2, miR-19b, -122, -182, -193a-3p, -195, and -224, as well as LncRNA MALAT1, TP73-AS1 and HOTTIP modulate proliferation, apoptosis, invasion, metastasis, or EMT via PI3K/ AKT pathway [260][261][262][263][264][265][266][267][268][269][270][271][272]. However, only a few of clinical trials of PI3K/AKT inhibitors in touch try to offer hope for KC patients (Tables 2 and 3).…”

Section: Dysregulation Of the Pi3k/akt Pathway In The Genitourinary Smentioning

confidence: 99%

Role of PI3K/AKT pathway in cancer: the framework of malignant behavior

et al. 2020

View full text Add to dashboard Cite

Given that the PI3K/AKT pathway has manifested its compelling influence on multiple cellular process, we further review the roles of hyperactivation of PI3K/AKT pathway in various human cancers. We state the abnormalities of PI3K/AKT pathway in different cancers, which are closely related with tumorigenesis, proliferation, growth, apoptosis, invasion, metastasis, epithelial-mesenchymal transition, stem-like phenotype, immune microenvironment and drug resistance of cancer cells. In addition, we investigated the current clinical trials of inhibitors against PI3K/AKT pathway in cancers and found that the clinical efficacy of these inhibitors as monotherapy has so far been limited despite of the promising preclinical activity, which means combinations of targeted therapy may achieve better efficacies in cancers. In short, we hope to feature PI3K/ AKT pathway in cancers to the clinic and bring the new promising to patients for targeted therapies.

show abstract

“…Notably, the present study demonstrated that miR-195 suppressed VEGFR2 protein expression in ovarian cancer cells. Sun et al (14) demonstrated that miR-195 suppresses angiogenesis and metastasis of hepatocellular carcinoma through VEGF expression. Wang et al (20) reported that miR-195-5p inhibits tumorigenesis of gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, AKT activation can inhibit ovarian cancer cell apoptosis (11) and is involved in the genesis, development, invasion and metastasis of ovarian cancer (13). Sun et al (14) indicate that miR-195 suppressed cell growth in renal cell…”

Section: Introductionmentioning

confidence: 99%

miRNA‑195 suppresses cell proliferation of ovarian cancer cell by regulating VEGFR2 and AKT signaling pathways

Chen

2018

Mol Med Report

View full text Add to dashboard Cite

The present study aimed to investigate the functional effects of microRNA‑195 on ovarian cancer cells and the underling mechanism involved. Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression of microRNA‑195 in patients with ovarian cancer. Cell proliferation and apoptosis were measured with MTT assay and flow cytometry, respectively. Caspase‑3/9 activity, vascular endothelial growth factor receptor (VEGFR)2 and phosphorylated protein kinase B (p‑AKT) protein expression were analyzed using caspase‑3/9 activity kits and western blot analysis. The expression of microRNA‑195 was downregulated in ovarian cancer, compared with the normal control group. Furthermore, microRNA‑195 suppresses cell proliferation and induced apoptosis of ovarian cancer cells. In addition, microRNA‑195 suppressed VEGFR2 and p‑AKT protein expression in ovarian cancer cells. The inhibition of VEGFR2 and p‑AKT increased the functional effects of microRNA‑195 on apoptosis of ovarian cancer cells. The results demonstrated that microRNA‑195 suppresses cell proliferation of ovarian cancer cells through regulation of VEGFR2 and AKT signaling pathways.

show abstract

MicroRNA-195 targets VEGFR2 and has a tumor suppressive role in ACHN cells via PI3K/Akt and Raf/MEK/ERK signaling pathways

Cited by 32 publications

References 34 publications

MicroRNA‑195 is associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinoma

MicroRNA‑195 is associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinoma

Role of PI3K/AKT pathway in cancer: the framework of malignant behavior

miRNA‑195 suppresses cell proliferation of ovarian cancer cell by regulating VEGFR2 and AKT signaling pathways

Contact Info

Product

Resources

About