Microfluidic wound bandage: Localized oxygen modulation of collagen maturation

Lo, Joe F.; Brennan, Martin D.; Merchant, Zameer; Lin, Chen; Guo, Shujuan; Eddington, David T.; DiPietro, Luisa A.

doi:10.1111/wrr.12021

Cited by 28 publications

(25 citation statements)

References 41 publications

(40 reference statements)

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“…A study by Fries et al, using topically applied pure oxygen on open wound sites in pigs showed that oxygen partial pressure (pO 2 ), when measured at 2 mm below the surface of the wound, increased from a baseline of 5–7 mmHg to levels >40 mmHg as early as 4 minutes into treatment, along with increases in vascular endothelial growth factor (VEGF) expression and formation of new blood vessels. This result was an important proof of principle that TOT can oxygenate superficial wound tissues, which was also supported by the studies of several other groups . An additional boost to TOT for wound healing comes from the decision of the United States FDA's proposal to reclassify TO devices from the most stringent class III (premarket approval) to a safer class II (http://www.fda.gov/cdrh/ode/guidance/1582.html).…”

Section: Introductionmentioning

confidence: 62%

Peroxide‐based oxygen generating topical wound dressing for enhancing healing of dermal wounds

Chandra

Ross

Smith

et al. 2015

Wound Repair Regeneration

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Oxygen generating biomaterials represent a new trend in regenerative medicine that aims to generate and supply oxygen at the site of requirement, to support tissue healing and regeneration. To enhance the healing of dermal wounds, we have developed a highly portable, in situ oxygen generating wound dressings that uses sodium percarbonate (SPO) and calcium peroxide (CPO) as chemical oxygen sources. The dressing continuously generated oxygen for more than 3 days, after which it was replaced. In the in vivo testing on porcine full-thickness porcine wound model, the SPO/CPO dressing showed enhanced wound healing during the 8 week study period. Quantitative measurements of wound healing related parameters, such as wound closure, reepithelialization, epidermal thickness and collagen content of dermis showed that supplying oxygen topically using the SPO/CPO dressing significantly accelerated the wound healing. An increase in neovascularization, as determined using Von Willebrand factor (vWF) and CD31 staining, was also observed in the presence of SPO/CPO dressing. This novel design for a wound dressing that contains oxygen generating biomaterials (SPO/CPO) for supplying topical oxygen, may find utility in treating various types of acute to chronic wounds.

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Section: Introductionmentioning

confidence: 62%

Peroxide‐based oxygen generating topical wound dressing for enhancing healing of dermal wounds

Chandra

Ross

Smith

et al. 2015

Wound Repair Regeneration

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“…In another mice study by Lo et al, TWO 2 therapy showed no difference in closure rate between oxygen‐treated and nontreated diabetic wounds . Both studies used different types of oxygen delivery devices, which may have affected the outcomes.…”

Section: Resultsmentioning

confidence: 99%

“…A total of ten studies were included which examined the use of TWO 2 on skin wounds. [27][28][29][30][31][32][33][34][35][36] Seven studies tested this therapy for chronic wounds, two studies tested this therapy for acute wounds and one study tested TWO 2 for chronic wounds as well as for acute wounds. Seven studies had at least one or more significant positive outcomes.…”

Section: Topical Oxygen Therapymentioning

confidence: 99%

Oxygen therapies and their effects on wound healing

Smet

Kroese

Menon

et al. 2017

Wound Repair Regeneration

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Oxygen is an important factor for wound healing. Although several different therapies investigated the use of oxygen to aid wound healing, the results of these studies are not unequivocal. This systematic review summarizes the clinical and experimental studies regarding different oxygen therapies for promoting wound healing, and evaluates the outcomes according the methodological details. A systematic literature search was conducted using Embase, Medline, Web of Science, Cochrane, PubMed publisher, and Google Scholar libraries. Clinical and experimental studies investigating oxygen for wound healing were selected. Included articles were categorized according to the kind of therapy, study design, and wound type. The methodological details were extracted and analyzed. Sixty-five articles were identified and divided in three different oxygen therapies: Local oxygen therapy, hyperbaric oxygen therapy, and supplemental inspired oxygen therapy. More than half of the included local oxygen and hyperbaric oxygen studies had one or more significant positive outcomes, 77 and 63%, respectively. Supplemental inspired oxygen therapy during gastrointestinal and vascular surgery was more likely to have a positive result than during other surgical interventions reducing surgical site infections. These many positive outcomes promote the use of oxygen treatment in the stimulation of wound healing. However, the lack of clinical studies and vast methodological diversity made it impossible to perform a proper comparison within and between the different therapies. Further randomized clinical studies are warranted to examine the value of these therapies, especially studies that investigate the more patient-friendly oxygen dressings and topical wound oxygen therapies. Also, to achieve more solid and consistent data, studies should use more standardized methods and subjects.

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“…For example, the use of oxygen under pressure or "hyperbaric oxygen therapy" (HBO 2 ) was shown to have a positive effect on ischemia and local wound hypoxia (4)(5)(6). In the last few years, additional medical procedures that allow topical administration of oxygen therapy directly to the wound site in order to support wound healing have been developed (7)(8)(9). One of the major complications affecting wound healing are infection, many of which are caused by multidrug-resistant pathogens and surface-associated (biofilm) bacteria (1,(10)(11)(12).…”

mentioning

confidence: 99%

Measurement of Predation and Biofilm Formation under Different Ambient Oxygen Conditions Using a Simple Gasbag-Based System

Kadouri

Tran

2013

Appl Environ Microbiol

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Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are Gram-negative bacteria characterized by predatory behavior. The aim of this study was to evaluate the ability of the predators to prey in different oxygen environments. When placed on an orbital shaker, a positive association between the rate of aeration and predation was observed. To further examine the effects of elevated ambient oxygen levels on predation, a simple gasbag system was developed. Using the system, we were able to conduct experiments at ambient oxygen levels of 3% to 86%. When placed in gasbags and inflated with air, 50% O 2 , and 100% O 2 , positive predation was seen on both planktonic and biofilm-grown prey cells. However, in low-oxygen environments, predatory bacteria were able to attack only prey cells grown as biofilms. To further evaluate the gasbag system, biofilm development of Grampositive and Gram-negative microorganisms was also measured. Although the gasbag system was found to be suitable for culturing bacteria that require a low-oxygen environment, it was not capable of supporting, with its current configuration, the growth of obligate anaerobes in liquid or agar medium.A cute and chronic wounds are a major burden to public health and the economy (1). It is estimated that, in the United States alone, chronic wounds affect 6.5 million patients (2). One medical procedure used to promote wound healing is the use of oxygen therapy (3). For example, the use of oxygen under pressure or "hyperbaric oxygen therapy" (HBO 2 ) was shown to have a positive effect on ischemia and local wound hypoxia (4-6). In the last few years, additional medical procedures that allow topical administration of oxygen therapy directly to the wound site in order to support wound healing have been developed (7-9). One of the major complications affecting wound healing are infection, many of which are caused by multidrug-resistant pathogens and surface-associated (biofilm) bacteria (1, 10-12). It was previously suggested that predatory bacteria from the genus Bdellovibrio and Micavibrio could be used as live antibiotics to control wound infections (13,14).The purpose of this study was to evaluate the ability of predatory bacteria to prey in elevated ambient O 2 levels with the longterm goal of using predatory bacteria in combination with traditional oxygen therapy in order to enhance wound healing. To this end, a new gasbag system that allowed experiments to be conducted in diverse gas environments was developed. The system was also used to evaluate biofilm formation of medically relevant Gram-positive and Gram-negative bacteria in O 2 environments that were not examined previously. MATERIALS AND METHODSPredatory bacteria. The predatory bacteria used in this study were Micavibrio aeruginosavorus ARL-13 (15, 16), Bdellovibrio bacteriovorus 109J (ATCC 43826), B. bacteriovorus HD100 (17, 18), and a previously isolated host-independent (HI) variant of B. bacteriovorus 109J (19). The HI variant was cultured at 30°C in peptone-yeast extract (PYE) (10 g/liter p...

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Microfluidic wound bandage: Localized oxygen modulation of collagen maturation

Cited by 28 publications

References 41 publications

Peroxide‐based oxygen generating topical wound dressing for enhancing healing of dermal wounds

Peroxide‐based oxygen generating topical wound dressing for enhancing healing of dermal wounds

Oxygen therapies and their effects on wound healing

Measurement of Predation and Biofilm Formation under Different Ambient Oxygen Conditions Using a Simple Gasbag-Based System

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