C1 metabolism and the <scp>C</scp>alvin cycle function simultaneously and independently during <scp>HCHO</scp> metabolism and detoxification in <i><scp>A</scp>rabidopsis thaliana</i> treated with <scp>HCHO</scp> solutions

Song, Zhiwei; Xiao, Shuqi; You, Liangzhi; Wang, Shasha; Tan, Hao; Li, Kunzhi; Chen, Limei

doi:10.1111/pce.12079

Cited by 30 publications

(47 citation statements)

References 56 publications

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“…CO 2 can be assimilated into sugars through the Calvin Cycle. The function of the Calvin Cycle during HCHO metabolism in plants has also been verified by some previous studies (Giese et al, 1994;Song et al, 2013;Schmitz et al, 2000).…”

Section: Introductionsupporting

confidence: 76%

“…In methylotroph, HCHO can directly bind with THF to form an adduct, which has been verified through in vitro reaction (Kallen and Jencks, 1966). The direct adduction of THF with HCHO was postulated to occur in Arabidopsis under a higher (6 mM) level of liquid HCHO stress (Song et al, 2013). During the entire period of 2 mM liquid HCHO stress, no obvious H 13 COOH signal peak or enhancement in [2-13 C]Gly signal peak were observed in petunia.…”

Section: The Met Generation Pathway In C1 Metabolism Played An Importmentioning

confidence: 99%

“…Cyclosporin A (CSA) or L-carnitine (LC) can specifically inhibit the opening of MPTPs in animal cells (Teng et al, 2001). The previous study (Song et al, 2013) …”

Section: Generation Of [5-13 C]met In Petunia Under a Low Level Of LImentioning

confidence: 99%

“…Formaldehyde (HCHO) is a key intermediate in plant one-carbon (C1) metabolism and some previous studies have shown that plants have ability to metabolize exogenous HCHO (Giese et al, 1994;Song et al, 2013;Song et al, 2010;Schmitz et al, 2000). A study using gaseous H 14 CHO-traced spider plants (Chlorophytum comosum L.) reported that the radioactivity from the gaseous H 14 CHO was integrated into organic acids, amino acids, free sugars, lipids and cell wall ingredients (Giese et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…However, HCHO metabolism in plants has only been investigated by a few studies (Giese et al, 1994;Song et al, 2013;Song et al, 2010;Schmitz et al, 2000). According to the results reported by these studies, it can be hypothesized that exogenous HCHO might be metabolized via C1 metabolism in plants through the following pathways (Hanson and Roje, 2001).…”

Section: Introductionmentioning

confidence: 99%

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C1 metabolism plays an important role during formaldehyde metabolism and detoxification in petunia under liquid HCHO stress

Zhang

Tang

Sun

et al. 2014

Plant Physiology and Biochemistry

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Section: Introductionsupporting

confidence: 76%

Section: The Met Generation Pathway In C1 Metabolism Played An Importmentioning

confidence: 99%

“…Cyclosporin A (CSA) or L-carnitine (LC) can specifically inhibit the opening of MPTPs in animal cells (Teng et al, 2001). The previous study (Song et al, 2013) …”

Section: Generation Of [5-13 C]met In Petunia Under a Low Level Of LImentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

C1 metabolism plays an important role during formaldehyde metabolism and detoxification in petunia under liquid HCHO stress

Zhang

Tang

Sun

et al. 2014

Plant Physiology and Biochemistry

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Investigation of the Role of the Calvin Cycle and C1 Metabolism during HCHO Metabolism in Gaseous HCHO-Treated Petunia under Light and Dark Conditions Using¹³C-NMR

et al. 2015

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Over-expression of the Arabidopsis formate dehydrogenase in chloroplasts enhances formaldehyde uptake and metabolism in transgenic tobacco leaves

Wang

Zeng

Guo

et al. 2017

Planta

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Over-expression of AtFDH controlled by the promoter of Rubisco small subunit in chloroplasts increases formaldehyde uptake and metabolism in tobacco leaves. Our previous study showed that formaldehyde (HCHO) uptake and resistance in tobacco are weaker than in Arabidopsis. Formate dehydrogenase in Arabidopsis (AtFDH) is a key enzyme in HCHO metabolism by oxidation of HCOOH to CO, which enters the Calvin cycle to be assimilated into glucose. HCHO metabolic mechanism in tobacco differs from that in Arabidopsis. In this study, AtFDH was over-expressed in the chloroplasts of transgenic tobacco using a light inducible promoter. C-NMR analysis showed that the carbon flux from HCHO metabolism was not introduced into the Calvin cycle to produce glucose in transgenic tobacco leaves. However, the over-expression of AtFDH significantly enhanced the HCHO metabolism in transgenic leaves. Consequently, the productions of [4-C]Asn, [3-C]Gln, [U-C]oxalate, and HCOOH were notably greater in transgenic leaves than in non-transformed leaves after treatment with HCHO. The increased stomatal conductance and aperture in transgenic leaves might be ascribed to the increased yield of oxalate in the guard cells with over-expressed AtFDH in chloroplasts. Accordingly, the transgenic plants exhibited a stronger capacity to absorb gaseous HCHO. Furthermore, the higher proline content in transgenic leaves compared with non-transformed leaves under HCHO stress might be attributable to the excess formate accumulation and Gln production. Consequently, the HCHO-induced oxidative stress was reduced in transgenic leaves.

show abstract

C1 metabolism and the Calvin cycle function simultaneously and independently during HCHO metabolism and detoxification in Arabidopsis thaliana treated with HCHO solutions

Cited by 30 publications

References 56 publications

C1 metabolism plays an important role during formaldehyde metabolism and detoxification in petunia under liquid HCHO stress

C1 metabolism plays an important role during formaldehyde metabolism and detoxification in petunia under liquid HCHO stress

Investigation of the Role of the Calvin Cycle and C1 Metabolism during HCHO Metabolism in Gaseous HCHO-Treated Petunia under Light and Dark Conditions Using¹³C-NMR

Over-expression of the Arabidopsis formate dehydrogenase in chloroplasts enhances formaldehyde uptake and metabolism in transgenic tobacco leaves

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C1 metabolism and the Calvin cycle function simultaneously and independently during HCHO metabolism and detoxification in Arabidopsis thaliana treated with HCHO solutions

Cited by 30 publications

References 56 publications

C1 metabolism plays an important role during formaldehyde metabolism and detoxification in petunia under liquid HCHO stress

C1 metabolism plays an important role during formaldehyde metabolism and detoxification in petunia under liquid HCHO stress

Investigation of the Role of the Calvin Cycle and C1 Metabolism during HCHO Metabolism in Gaseous HCHO-Treated Petunia under Light and Dark Conditions Using13C-NMR

Over-expression of the Arabidopsis formate dehydrogenase in chloroplasts enhances formaldehyde uptake and metabolism in transgenic tobacco leaves

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Investigation of the Role of the Calvin Cycle and C1 Metabolism during HCHO Metabolism in Gaseous HCHO-Treated Petunia under Light and Dark Conditions Using¹³C-NMR