Variant of the Thermomyces lanuginosus lipase with improved kinetic stability: A candidate for enzyme replacement therapy

Wang, H.; Hagedorn, J; Svendsen, Allan; Borch, Kim; Otzen, Daniel E.

doi:10.1016/j.bpc.2012.12.003

Cited by 9 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BAs also influence the stability of other fungal metabolites. For example, luminal BAs 43 influence the stability of a prominent lipase in Thermomyces, which we observed as the taxa most significantly associated with weight gain on a processed diet. Intriguingly, in our model, these differences correlated with differences in metabolic hormones, including leptin, resistin, and ghrelin.…”

Section: Discussionmentioning

confidence: 67%

The gut mycobiome of healthy mice is shaped by the environment and correlates with metabolic outcomes in response to diet

et al. 2021

View full text Add to dashboard Cite

As an active interface between the host and their diet, the gut microbiota influences host metabolic adaptation; however, the contributions of fungi have been overlooked. Here, we investigate whether variations in gut mycobiome abundance and composition correlate with key features of host metabolism. We obtained animals from four commercial sources in parallel to test if differing starting mycobiomes can shape host adaptation in response to processed diets. We show that the gut mycobiome of healthy mice is shaped by the environment, including diet, and significantly correlates with metabolic outcomes. We demonstrate that exposure to processed diet leads to persistent differences in fungal communities that significantly associate with differential deposition of body mass in male mice compared to mice fed standardized diet. Fat deposition in the liver, transcriptional adaptation of metabolically active tissues and serum metabolic biomarker levels are linked with alterations in fungal community diversity and composition. Specifically, variation in fungi from the genera Thermomyces and Saccharomyces most strongly associate with metabolic disturbance and weight gain. These data suggest that host–microbe metabolic interactions may be influenced by variability in the mycobiome. This work highlights the potential significance of the gut mycobiome in health and has implications for human and experimental metabolic studies.

show abstract

Section: Discussionmentioning

confidence: 67%

The gut mycobiome of healthy mice is shaped by the environment and correlates with metabolic outcomes in response to diet

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The problem with human lipases is that they get easily deactivated by proteases; therefore, for preparations containing this kind of lipase, it is advisable to use a reduced amount of protease to ensure the survival of the lipases [ 30 ]. Efficacy can be further enhanced by the use of various fungal enzymes (e.g., lipase from Rhizopus arrhizus , Aspergillus niger , Rhizopus oryzae , and Aspergillus oryzae ) [ 31 , 32 , 33 ] and bacterial enzymes (e.g., lipase from Burkholderia cepacia and Burkholderia plantarii ) [ 34 , 35 ]. Fungal enzymes are known to be significantly less stable than bacterial ones [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Formulation of Cyclodextrin Stabilized Lipases for Efficient Pancreatin Replacement Therapies

et al. 2021

View full text Add to dashboard Cite

Enzyme replacement therapies (ERT) have been of great help over the past 30 years in the treatment of various lysosomal storage disorders, including chronic pancreatitis and its common complication, exocrine pancreatic insufficiency. Research shows that difficulties in designing such drugs can be overcome by using appropriate additives and various enzyme immobilization techniques. Cyclodextrins (CDs) can be considered as a promising additive for enzyme replacement therapies, as they are known to enhance the activity of enzymes in a complex process due to their specific binding. In this study, we investigated the formulation of lipases (from Aspergillus oryzae and Burkholderia cepacia) paired with different cyclodextrins in poly(vinyl alcohol) (PVA) nanofibers by electrospinning technique. We examined the effect of the presence of cyclodextrins and nanoformulation on the lipase activity. The rheological and morphological characterizations of precursors and nanofibers were also performed using a viscometer as well as electron and Raman microscope. We found that by selecting the appropriate CD:lipase ratio, the activity of the investigated enzyme could be multiplied, and cyclodextrins can support the homogeneous dispersion of lipases inside the solid formula. In addition, the entrapment of lipases in PVA nanofibers led to a significant increase in activity compared to the preformulated precursor. In this way, the nanofibrous formulation of lipases combining CDs as additives can provide an efficient and sustainable possibility for designing novel solid medicines in ERT.

show abstract

Stability of Therapeutic Enzymes: Challenges and Recent Advances

Ghosh

Alam

Rathore

et al. 2019

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Variant of the Thermomyces lanuginosus lipase with improved kinetic stability: A candidate for enzyme replacement therapy

Cited by 9 publications

References 54 publications

The gut mycobiome of healthy mice is shaped by the environment and correlates with metabolic outcomes in response to diet

The gut mycobiome of healthy mice is shaped by the environment and correlates with metabolic outcomes in response to diet

Nanofibrous Formulation of Cyclodextrin Stabilized Lipases for Efficient Pancreatin Replacement Therapies

Stability of Therapeutic Enzymes: Challenges and Recent Advances

Contact Info

Product

Resources

About