Induction of Periimplantitis in Dental Implants

Becker, Stephan; Föge, Marc; Beck‐Broichsitter, Benedicta; Гаврилова, Ольга Анатольевна; Bolte, Hendrik; Rosenstiel, Philip; Wiltfang, Jens

doi:10.1097/scs.0b013e318266fb2d

Cited by 22 publications

(34 citation statements)

References 13 publications

(10 reference statements)

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“…Although information can be gained by Baker’s model, it does not resemble clinical implant placement in humans. Nonetheless, our results corroborate those of Becker et al in that they confirm that a mouse model can be effective in the investigation of the pathogenesis of peri-implant mucositis and peri-implantitis (Becker, Foge et al 2013). Other disease models, such as ligature-induced peri-implantitis are under investigation by our research group.…”

Section: Discussionsupporting

confidence: 92%

A Murine Model of Lipopolysaccharide-Induced Peri-Implant Mucositis and Peri-Implantitis

Pirih

Hiyari

Leung

et al. 2015

Journal of Oral Implantology

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Introduction Dental implants are a vastly used treatment option for tooth replacement. Dental implants are however susceptible to inflammatory diseases such as peri-implant mucositis and peri-implantitis, which are highly prevalent and may lead to implant loss. Unfortunately, the understanding of the pathogenesis of peri-implant mucositis and peri-implantitis is fragmented and incomplete. Therefore, the availability of a reproducible animal model to study these inflammatory diseases would facilitate the dissection of their pathogenic mechanisms. The objective of this study is to propose a murine model of experimental peri-implant mucositis and peri-implantitis. Materials and Methods Screw-shaped titanium implants were placed in the upper healed edentulous alveolar ridges of C57BL/6J mice eight weeks after tooth extraction. Following four weeks of osseointegration, Porphyromonas gingivalis-lipolysaccharide (LPS) injections were delivered to the peri-implant soft tissues for six weeks. No-injections and vehicle injections were utilized as controls. Peri-implant mucositis and peri-implantitis were assessed clinically, radiographically (micro-CT) and histologically following LPS-treatment. Results LPS-injections resulted in a significant increase in soft tissue edema around the head of the implants as compared to the control groups. Micro-CT analysis revealed significantly greater bone loss in the LPS-treated implants. Histological analysis of the specimens demonstrated that the LPS-group had increased soft tissue vascularity, which harbored a dense mixed inflammatory cell infiltrate, and the bone exhibited noticeable osteoclast activity. Conclusion The induction of peri-implant mucositis and peri-implantitis in mice via localized delivery of bacterial LPS has been demonstrated. We anticipate that this model will contribute to the development of more effective preventive and therapeutic approaches for these two conditions.

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Section: Discussionsupporting

confidence: 92%

A Murine Model of Lipopolysaccharide-Induced Peri-Implant Mucositis and Peri-Implantitis

Pirih

Hiyari

Leung

et al. 2015

Journal of Oral Implantology

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“…The main difference between our model and the one described by Becker et al . is that theirs included placement of the implant in the central palatal bone and not in an area previously occupied by a tooth, which does not fully mimic implants placed in humans.…”

Section: Discussionmentioning

confidence: 99%

“…Mice constitute an attractive option in this regard, not only because they fit the criteria described above but also because of the availability of genetically manipulated strains, which are invaluable tools in dissecting the mechanisms of diseases. Only one murine model for peri‐implantitis has been described thus far; however, in that model, implants were placed in the center of the palate and not in the alveolar bone.…”

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confidence: 99%

Ligature‐induced peri‐implantitis in mice

Pirih

Hiyari

Barroso

et al. 2014

J of Periodontal Research

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Introduction Peri-implantitis has a prevalence of 11-47%, involves destruction of peri-implant bone, and may lead to implant loss. A detailed understanding of the pathogenesis of peri-implantitis is lacking. The objective of this study was to develop a murine model of experimental peri-implantitis. Materials and Methods Machined, smooth surface screw-shaped titanium implants were placed in the healed alveolar bone of the left maxillary molars of C57BL/6J male mice, eight weeks after tooth extraction. Peri-implantitis was induced by securing silk ligatures around the head of the implant fixtures. Implant survival and peri-implant bone levels were analyzed by micro-computerized tomography (micro-CT) scans and histology twelve weeks after ligature placement. Results Implant survival was 60% (6/10) for implants with ligatures and 100% (8/8) for controls. Micro-CT revealed significantly greater bone loss around the implants that received ligatures and that survived as compared to controls. The radiographic findings were confirmed via histology and toluidine blue staining. Conclusions This study describes a murine model of experimental peri-implantitis around screw-shaped titanium implants placed in the edentulous alveolar bone. This model should be a useful tool to dissect pathogenic mechanisms of peri-implantitis and evaluate potential treatment interventions.

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“…Während bei der Periimplantitis hauptsächlich Transkripte aus dem Bereich der angeborenen Immunität dominieren, herrschen bei Patienten mit Parodontitis solche vor, die als Reaktion auf eine bakterielle Antwort aktiviert werden. Weitere Untersuchungen am erstmalig etablierten Mausmodell werden folgen [17]. Peri implantitis kann auch durch das Vorhandensein iatrogener Faktoren, wie Zementreste, Überkonturierung prothetischer Restaurationen, Fehlpositionierung des Implantats und technische Komplika tionen) verursacht werden [7].…”

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