The cytopathic Effects of Trichomonas vaginalis on Fibroblast Cell Culture Alone and with C. albicans and E. coli

Abstract

Objective: In this study, the cytopathic effects of Trichomonas vaginalis were investigated in L929 mouse fibroblast cell cultures (FCC) under different conditions: only parasite, or coexistence with Candida albicans and Escherichia coli. Methods: The parasite was isolated from a symptomatic patient and cultured in Cysteine-Peptone-Liver infusion Maltose medium (CPLM). C. albicans strain 10235 and E. coli strain 25922 were used in the experiments. Five groups were created and inoculated on FCC. The groups were as follows; only T. vaginalis, C. albicans, E. coli, T. vaginalis+C. albicans and T. vaginalis+E. coli. The plates were incubated for 24 hours and cell viability was examined under an inverted microscope. Each experiment was repeated 11 times. Results: The fibroblast death rate was 19.1%, 21%, 40.9%, 96.5% and 89.6% in the five groups, respectively. Conclusion: All fibroblasts were alive in the control group. T. vaginalis showed almost 100% cytopathic effects on FCC with C. albicans and parasites were very motile in this coexistence. (Turkiye Parazitol Derg 2012; 36: 193-

Analysis of Volatile Constituent by Hydrodistillation and Solid‐Phase Microextraction Techniques and Antimicrobial and Scolicidal Activities of Essential Oil and Soxhlet Extracts of Ulva rigida grown in Turkey

“…Özcelik et al. (2007) reported that Allium sativum was effective on protoscoleces at 50 mg/mL concentration of at the 15th min [42] . Özcelik et al (2015) also determined that propolis can act on daughter vesicles within 10 min [43] .…”

Comparison of volatile chemical components of Cystoseira crinita Duby by hydrodistillation(HD) and solid-phase microextraction (SPME) methods and antimicrobialand scolicidal activities of essential oil and extracts

“…Ozcelik et al [ 53 ] reported that Allium sativum was effective on protoscolices. Again, Ozcelik et al [ 54 ] found that propolis also affected daughter vesicles within 10 min.…”