First 2-DE approach towards characterising the proteome and immunome of Besnoitia besnoiti in the tachyzoite stage

García-Lunar, Paula; Regidor‐Cerrillo, Javier; Gutiérrez-Expósito, Daniel; Ortega‐Mora, Luis Miguel; Álvarez‐García, Gema

doi:10.1016/j.vetpar.2012.12.040

Cited by 14 publications

(12 citation statements)

References 53 publications

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“…However, the disease molecular mechanisms and parasite biology have still to be clarified. García-Lunar et al [ 31 ] conducted a proteomic study to describe the proteome and immunome of the tachyzoite stage of BB using 2-DE, immunoblotting and MALDI-TOF/TOF MS.…”

Section: Animal Serum Proteomic Applicationsmentioning

confidence: 99%

Farm Animal Serum Proteomics and Impact on Human Health

Girolamo

D’Amato

Lante³

et al. 2014

IJMS

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Due to the incompleteness of animal genome sequencing, the analysis and characterization of serum proteomes of most farm animals are still in their infancy, compared to the already well-documented human serum proteome. This review focuses on the implications of the farm animal serum proteomics in order to identify novel biomarkers for animal welfare, early diagnosis, prognosis and monitoring of infectious disease treatment, and develop new vaccines, aiming at determining the reciprocal benefits for humans and animals.

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Section: Animal Serum Proteomic Applicationsmentioning

confidence: 99%

Farm Animal Serum Proteomics and Impact on Human Health

Girolamo

D’Amato

Lante³

et al. 2014

IJMS

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“…In Sarcocystidae parasites, the bradyzoite differentiation is an immune evasion mechanism that allows the parasite to persist in the host for a long life (Hemphill & Gottstein, ; Jeffers, Tampaki, Kim, & Sullivan, ). Indeed, B. besnoiti tachyzoites display different antigenic patterns and protein compositions compared with bradyzoites (Fernández‐García et al, ; García‐Lunar, Regidor‐Cerrillo, Gutiérrez‐Expósito, Ortega‐Mora, & Álvarez‐García, ). Moreover, in the closely related N. caninum, bradyzoite antigens are less immunogenic compared with tachyzoite antigens (Jiménez‐Ruíz et al, ).…”

Section: Discussionmentioning

confidence: 99%

A model for chronic bovine besnoitiosis: Parasite stage and inoculation route are key factors

Diezma-Díaz

Ferre

et al. 2019

Transbound Emerg Dis

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In this work, an experimental model for chronic besnoitiosis in bovine was developed and characterized. Using a previously established calf model, two new variables (parasite stage and inoculation route) were combined and used. Twelve Holstein Friesian 3‐month‐old male calves were randomly divided into four groups of three animals each. Bradyzoites were obtained from a chronically infected bull and used for inoculation via three different inoculation routes. Three groups were inoculated with 106 bradyzoites by intravenous (G1), subcutaneous (G2) and intradermal (G3) routes, and a non‐infected control group (G4) was inoculated with PBS. The trial lasted for 90 days and included daily clinical monitoring as well as weekly skin biopsies and blood sampling. Sera were obtained to analyse both cellular and humoral responses. Once the calves were euthanized, tissues from the skin, eyes, respiratory and reproductive tracts, among others, were collected to study presence of the parasite. Clinically, the infection was classified as mild to moderate for the acute stage since all infected calves showed lymphadenopathy from four days post‐infection (pi) and fever from one week pi until 24 days pi. However, the most relevant results were achieved during the chronic stage that was classified as moderate to severe. In fact, pathognomonic conjunctival cysts were observed in all infected calves from 40 days pi onwards and were more abundant in G3. Moreover, one calf from this group developed skin lesions (49 days pi). The microscopic tissue cysts and Besnoitia DNA were detected primarily in skin, reproductive tract and respiratory tissue samples, and parasite load was higher in G3. In conclusion, the parasite stage (bradyzoite) and the inoculation route are key factors that influence the outcome of an infection. In particular, the intradermal route led to more severe clinical signs of the chronic phase in the inoculated calves.

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“…This has led to the development of several methods, from the detection of the total protein pattern on the 2D blots prior and/or after the immunodetection (e.g., in [ 64 ]) to the partial blotting process, in which the very same 2D gel is used, both for generating the 2D reference pattern for MS identification and the 2D blot for immunodetection [ 65 ]. In the immune responses to pathogens, work has been carried out on bacteria [ 66 , 67 , 68 , 69 , 70 ], fungi [ 71 , 72 , 73 ], and parasites [ 74 , 75 ]. Such studies have always reached their initial goal of providing the major immunogens from the pathogen under study, and have sometimes resulted in valuable clinical advances [ 71 , 76 , 77 ].…”

Section: When An Unpredictable Subset Of Proteins Is To Be Analyzementioning

confidence: 99%

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

2020

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Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past. Although this opinion may be true for some biological questions, e.g., when analysis depth is of critical importance, for many others, two-dimensional gel electrophoresis-based proteomics still has a lot to offer. This is because of its robustness, its ability to separate proteoforms, and its easy interface with many powerful biochemistry techniques (including western blotting). This paper reviews where and why two-dimensional gel electrophoresis-based proteomics can still be profitably used. It emerges that, rather than being a thing of the past, two-dimensional gel electrophoresis-based proteomics is still highly valuable for many studies. Thus, its use cannot be dismissed on simple fashion arguments and, as usual, in science, the tree is to be judged by the fruit.

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First 2-DE approach towards characterising the proteome and immunome of Besnoitia besnoiti in the tachyzoite stage

Cited by 14 publications

References 53 publications

Farm Animal Serum Proteomics and Impact on Human Health

Farm Animal Serum Proteomics and Impact on Human Health

A model for chronic bovine besnoitiosis: Parasite stage and inoculation route are key factors

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

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