Farm Animal Serum Proteomics and Impact on Human Health

Girolamo, Francesco; D’Amato, Alfonsina; Lante, Isabella; Signore, Fabrizio; Muraca, Marta; Putignani, Lorenza

doi:10.3390/ijms150915396

Cited by 24 publications

(15 citation statements)

References 55 publications

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“…; Di Girolamo et al . ). Proteomic studies on fish welfare target mainly the liver, due to its central role in most key metabolic pathways related to the adaptive responses to stress.…”

Section: Proteomics As a Tool To Assess Fish Welfarementioning

confidence: 97%

“…In an effort to try to respond to this demand, as well as to produce a fish with an optimal growth rate and health status, researchers have started to approach this concept through more advanced high-throughput technologies (Marco-Ramell et al 2016). Proteomics emerged in aquaculture welfare studies as an extremely valuable tool to try to better understand fish welfare, improve welfare status assessment, optimise fish capacity to cope with unavoidable challenges/stress and find candidate stress biomarkers (Rodrigues et al 2012;Almeida et al 2014;Di Girolamo et al 2014). Proteomic studies on fish welfare target mainly the liver, due to its central role in most key metabolic pathways related to the adaptive responses to stress.…”

Section: Proteomics As a Tool To Assess Fish Welfarementioning

confidence: 99%

“…One of the main aims of proteomics in aquaculture welfare studies is to identify putative proteins as biological markers and stress status indicators (Di Girolamo et al 2014;Sanahuja & Ibarz 2015). Reliable biomarkers of fish welfare assessment would be very useful and complementary to behavioural observations and immunochemical tests based on secondary stress effects, such as the cortisol release and other molecules (Marco-Ramell et al 2016).…”

Section: Metabolic Indicators Of Fish Welfare: Biomarkersmentioning

confidence: 99%

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A Proteomics and other Omics approach in the context of farmed fish welfare and biomarker discovery

Magalhães

Cerqueira

Schrama

et al. 2018

Reviews in Aquaculture

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The rapid and intensive growth of aquaculture over the last decade, poses a tremendous challenge to this industry in order to comply with the latest guidelines, established to minimise its negative effects on the environment, animal welfare and public health. Farmed fish welfare has become one of the main priorities towards sustainable aquaculture production with several initiatives launched by the European Union within the framework of the 2030 agenda. It is clear that an unbiased and reliable way to access farmed fish welfare needs to be implemented due to the lack of reliable indicators and standardised methods that are used at present. In this review, we start by addressing the status quo of animal and fish welfare definition in particular, describing the methods and assays currently used to measure it. We then explain why we believe these methods are unreliable and why there is a need to establish new ones that will promote productivity and consumer's acceptance of farmed fish. The establishment of a new type of welfare biomarkers using cutting‐edge technologies like proteomics and other omics technologies is proposed as a solution to this issue. Therefore, we provide a brief description of these new methodologies, describing for each one how they can improve our scientific knowledge and the role they can play in farmed fish welfare biomarker discovery.

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“…; Di Girolamo et al . ). Proteomic studies on fish welfare target mainly the liver, due to its central role in most key metabolic pathways related to the adaptive responses to stress.…”

Section: Proteomics As a Tool To Assess Fish Welfarementioning

confidence: 97%

Section: Proteomics As a Tool To Assess Fish Welfarementioning

confidence: 99%

Section: Metabolic Indicators Of Fish Welfare: Biomarkersmentioning

confidence: 99%

See 1 more Smart Citation

A Proteomics and other Omics approach in the context of farmed fish welfare and biomarker discovery

Magalhães

Cerqueira

Schrama

et al. 2018

Reviews in Aquaculture

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“…Nevertheless, compared to humans, biomarker discovery studies directly performed on animal specimens lag considerably behind . This might be partly due to specific difficulties in treating samples and to the limited availability of annotated protein sequence databases . One of the main problems in proteomic analysis of biological specimens, such as plasma, serum, and urine, is the presence of a huge dynamic molecular range of protein concentration .…”

Section: Numbers Of Identified Proteins Peptides Psms and Search Imentioning

confidence: 99%

“…[3][4][5] This might be partly due to specific difficulties in treating samples and to the limited availability of annotated protein sequence databases. [6] One of the main problems in proteomic analysis of biological specimens, such as plasma, serum, and urine, is the presence of a huge dynamic molecular range of protein concentration. [7][8][9] The availability of commercial products aimed at depleting highly abundant protein and enriching the low abundant ones generally enables a more efficient and less challenging biomarker discovery process.…”

Section: Doi: 101002/pmic201800191mentioning

confidence: 99%

All Cats are Gray in the Dark: Enrichment/Depletion Approaches for Biomarker Discovery on Felis catus Plasma

et al. 2018

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In veterinary medicine, assay performance is often affected by the lack of species-specific diagnostic tools. Reliable biomarkers might be identified by investigating biological fluids of the species of interest, but protein sequence databases are often incomplete and human-specific devices for reducing sample complexity might fail when applied to animal plasma. Here, seven commercial methods based on different capturing agents (anti-human antibodies, affinity ligands, mixture of antibodies and ligands, and combinatorial peptide ligand libraries) are applied to cat plasma and evaluated in terms of yield, identified proteins/ peptides, and relative abundance by high-resolution shotgun proteomics and label-free quantitation. As a result, anti-human antibody-based methods are unsatisfactory. Most fail in reducing albumin and immunoglobulins, and some lead to a substantial removal of other highly abundant proteins, probably because of nonspecific interactions. A protein A/dye ligand-based method is efficient in reducing immunoglobulins, fibrinogen, and apolipoprotein A1 and A2, but not albumin, and protein identifications do not increase. Only peptide ligand libraries flatten the dynamic range, and increased protein identification (59.0%). Albumin and immunoglobulins are successfully depleted (60.7% and 35.9%, respectively). Although further studies will be required for reinforcing our observations, this work can provide a useful guide for cat plasma pretreatment in biomarker discovery studies.

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Plasma proteome of buffaloes

Pontes

Cavassan

Barros

et al. 2017

Proteomics Clinical Apps

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The proteomic approach has aroused the interest of veterinary medicine researchers, especially regarding the production of biopharmaceuticals and diagnosis of diseases in farm animals. Water buffaloes have gained prominence in the world economy due to the quality of their milk, meat, and leather, in addition to being an important donor of blood components. This work aimed to identify and characterize the proteins present in the blood plasma of Murrah buffaloes (Bubalus bubalis) through 2D electrophoresis, in gel protein digestion followed by mass spectrometry technique and for albumin depletion, in solution protein digestion followed by shotgun analysis. Our results showed the identification of 112 protein spots and 35 individual proteins, respectively. The abundant proteins were represented by albumin, fibrinogen-α, fibrinogen-β, fibrinogen-γ, immunoglobulins in general, α-1-antiproteinase, α-1B-glycoprotein, α-2-HS-glycoprotein, α-macroglobulin, apolipoprotein A1, antithrombin-III, endopin 2B, fetuin-B, retinol-binding protein, serotransferrin, transthyretin and vitamin D-binding protein. Most of these proteins are related to the signaling pathways of the complement system and coagulation cascade. The results allowed a better understanding of the protein composition of these blood components, thus promoting studies on animal health in the search for molecular markers of zoonotic diseases in buffaloes.

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Farm Animal Serum Proteomics and Impact on Human Health

Cited by 24 publications

References 55 publications

A Proteomics and other Omics approach in the context of farmed fish welfare and biomarker discovery

A Proteomics and other Omics approach in the context of farmed fish welfare and biomarker discovery

All Cats are Gray in the Dark: Enrichment/Depletion Approaches for Biomarker Discovery on Felis catus Plasma

Plasma proteome of buffaloes

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