Doxorubicin-Loaded MePEG-PCL Nanoparticles for Prevention of Posterior Capsular Opacification

Guha, Rajdeep; Chowdhury, Sushovan; Palui, Himangshu; Mishra, Akhilesh; Basak, Samar; Mandal, Tapan Kumar; Hazra, Sarbani; Konar, Aditya

doi:10.2217/nnm.12.175

Cited by 23 publications

(15 citation statements)

References 20 publications

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“…Acute inflammatory response of cornea may lead to retinal gliosis 48 which may alter retinal physiology; we therefore performed ERGs to evaluate any such effect following Am silk film implantation. Electroretinography was performed two months after implantation following standard procedure as described earlier 49 . In brief, the rabbits were dark adapted for 2 hours, mydriasis was achieved using 1% topicamide and 2.5% phenylephrine; rabbits were anesthetized with a combination of xylazine hydrochloride @ 5 mg/ Kg and ketamine hydrochloride @ 35 mg /Kg body weight and 0.5% proparacain hydrochloride was used for topical anesthesia.…”

Section: Methodsmentioning

confidence: 99%

Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration

Hazra

Nandi

Naskar

et al. 2016

Sci Rep

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Purpose: Successful repair of a damaged corneal surface is a great challenge and may require the use of a scaffold that supports cell growth and differentiation. Amniotic membrane is currently used for this purpose, in spite of its limitations. A thin transparent silk fibroin film from non-mulberry Antheraea mylitta (Am) has been developed which offers to be a promising alternative. The silk scaffolds provide sufficient rigidity for easy handling, the scaffolds support the sprouting, migration, attachment and growth of epithelial cells and keratocytes from rat corneal explants; the cells form a cell sheet, preserve their phenotypes, express cytokeratin3 and vimentin respectively. The films also support growth of limbal stem cell evidenced by expression of ABCG2. The cell growth on the silk film and the amniotic membrane is comparable. The implanted film within the rabbit cornea remains transparent, stable. The clinical examination as well as histology shows absence of any inflammatory response or neovascularization. The corneal surface integrity is maintained; tear formation, intraocular pressure and electroretinography of implanted eyes show no adverse changes. The silk fibroin film from non-mulberry silk worms may be a worthy candidate for use as a corneal scaffold.

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Section: Methodsmentioning

confidence: 99%

Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration

Hazra

Nandi

Naskar

et al. 2016

Sci Rep

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“…To put an end for these gaps and improve the oral efficacy of drugs, various approaches in the form of polymer prodrugs [ 9 , 10 ], polymer conjugates [ 11 , 12 ], liposomes [ 13 , 14 ], solid lipid NPs [ 15 , 16 ] and polymeric nanoparticles (NPs) [ 17 – 19 ], have been evaluated. The successful outcomes for such approaches have been observed in the shape of SLN for doxorubicin [ 20 , 21 ], layersomes for doxorubicin [ 22 ], SEDDS for etoposide [ 23 ], polymeric micelles for paclitaxel [ 24 ], dendrimer for doxorubicin [ 25 ] as well as polymeric NPs for etoposide and epirubicin respectively [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of oral bioavailability of doxorubicin through surface modified biodegradable polymeric nanoparticles

Ahmad

Alam

et al. 2018

Chemistry Central Journal

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BackgroundDoxorubicin hydrochloride (DOX·HCl), an anthracycline glycoside antibiotic, exhibits low oral bioavailability due to active efflux from intestinal P-glycoprotein receptors. The oral administration of DOX remains a challenge hence; no oral formulation for DOX is marketed, till date.Aim of the studyTo improve the oral bioavailability of DOX through, preparation of a nanoformulation i.e. PEGylated-doxorubicin(DOX)-loaded-poly-lactic-co-glycolic acid (PLGA)-Nanoparticles (NPs) and to develop and validate an ultra-high performance liquid chromatography electrospray ionization-synapt mass spectrometric bioanalytical method (UHPLC/ESI-QTOF–MS/MS) for plasma (Wistar rats) DOX quantification.Materials and methodsFor chromatography, Waters ACQUITY UPLC™ along with a BEH C-18 column (2.1 mm × 100 mm; 1.7 μm), mobile phase conditions (acetonitrile: 0.1% formic acid::1:1 v/v) and flow rate (0.20 ml/min) was used. For analyte recovery from rat plasma, a liquid–liquid extraction method (LLE), using Acetonitrile: 5 mM ammonium acetate in a ratio of 6:4 v/v at pH 3.5, was used.ResultsNanoformulation with a particle size (183.10 ± 7.41 nm), zeta potential (− 13.10 ± 1.04 mV), drug content (42.69 ± 1.97 µg/mg) and a spherical shape and smooth surface was developed. An elution time of 1.61 and 1.75 min along with a transition at m/z 544.42/397.27 and 528.46/321.41 were observed for DOX and internal standard (IS) Daunorubicin, respectively. In addition, a linear dynamic range with r2 ≥ 0.9985 over a concentration range of 1.00–2500.0 ng/ml was observed for different processes and parameters used in the study. Similarly a marked improvement i.e. 6.8 fold was observed, in PEGylated-DOX-PLGA-NPs as compared to DOX-S, in pharmacokinetics studies.ConclusionThe promising approach of PEGylated-DOX-PLGA-NPs may provide an alternate to intravenous therapy for better patient care.

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“…In this study, drug-loaded CHI nanoparticles were prepared by the “ionic gelation” method, having advantages of the electrostatic complexation of the cationic CHI and anionic TPP, in which TPP is the most classic ionic agent in the CHI nanoparticle preparation 34. The antiproliferative drug DOX, which has been previously investigated for PCO prevention, was added in the CHI solution to obtain the drug-loaded CHI nanoparticles 35,36. As shown in Figure 2A, the N/P ratio was critical to control the nanoparticle size and its distribution in the uncross-linked case.…”

Section: Resultsmentioning

confidence: 99%

<p>Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification</p>

Han

Tang

Xia

et al. 2019

IJN

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BackgroundPosterior capsular opacification (PCO) is the main complication after intraocular lens (IOL) implantation in cataract surgery, which is the result of lens epithelial cell (LEC) adhesion, proliferation and migration on the IOL and at the lens capsule interface. Hydrophilic surface modification, such as surface heparinization, decreases the cell adhesion, which has been commercialized and used clinically. However, clinical long-term observation results show no significant difference between the pristine and heparinized IOLs.MethodsTo prevent PCO over the long time span, we modified the IOLs with an antiproliferative drug-loaded hydrophilic coating. The antiproliferative drug doxorubicin (DOX)-incorporated chitosan (CHI) nanoparticle was fabricated by sodium tripolyphosphate (TPP) gelation. Such antiproliferative drug-loaded CHI-TPP-DOX nanoparticles (CTDNP) were used as one of the building blocks to prepare polyelectrolyte multilayer with heparin (HEP) via layer-by-layer assembly, obtaining (HEP/CTDNP)n multilayers. The assembly process was characterized by quartz crystal microbalance with dissipation (QCM-D). The drug release behavior of the coating was investigated by ultra-HPLC (UPLC). In vitro cell experiments were carried out to monitor the effects of multifunctional coatings on cellular adhesion, proliferation and migration. And the intraocular implantation was performed on rabbits to evaluate the in vivo PCO inhibitory effect of such surface-functionalized IOLs.ResultsThe positively charged CTDNP was successfully prepared by ionic gelation. The QCM-D results indicate the successful preparation of the (HEP/CTDNP)n multilayer film. Drug release profiles showed that surface-multifunctionalized IOL had drug-sustained release properties. In vitro cell culture results showed significant inhibition of adhesion, proliferation and migration of LECs after surface modification. The in vivo results showed that the IOLs with multifunctionalized surface can effectively reduce the posterior hyperplasia and Soemmering’s ring (SR) formation.ConclusionThese findings suggested that such multifunctionalized drug-eluting IOLs can effectively reduce the posterior hyperplasia and SR formation when intraocular implantation has a major impact on reducing PCO incidence. Thus they have a great potential in improving patient vision recovery and maintenance.

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Doxorubicin-Loaded MePEG-PCL Nanoparticles for Prevention of Posterior Capsular Opacification

Abstract: The authors conclude that DOX-loaded MePEG-PCL NPs show promise as a new approach to selectively kill highly proliferative lens epithelial cells in vivo following cataract surgery, while sparing normal tissue.

Cited by 23 publications

References 20 publications

Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration

Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration

Enhancement of oral bioavailability of doxorubicin through surface modified biodegradable polymeric nanoparticles

<p>Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification</p>

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