Gene Transfer into the Both Sides of Postnatal Cerebral Cortex Using Tweezer Electrode

Ding, Xuefeng; Zhao, Yong-Qi; Fan, Wenhong; Wu, Liying; Huang, Xin; Wang, Fei; He, Yun-Ling; Liu, Shuhong; Wu, Yan; Zhao, Tong; Wāng, Ying; Zhu, Ling‐Ling; Fan, Ming

doi:10.1111/cns.12056

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Cited by 2 publications

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“…Immediately after injection, five electric pulses at 90V (50 ms duration and 1s interval) were delivered to the operated head using BTX electroporator with tweezer type electrodes (7 mm diameter, BTX, Holliston, MA, USA). Finally, the successfully operated pups were placed on a heat pad until they recovered full mobility and subsequently returned to their mother [10]. The operated brain was examined with fluorescence microscope 24 h after electroporation, and the frozen sections (30 lm) were prepared.…”

mentioning

confidence: 99%

Specific Expression of Foreign Gene in Postnatal Mouse Glial Cells using Electroporation

Ding

Hu²,

Zhao

et al. 2013

CNS Neurosci Ther

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mentioning

confidence: 99%

Specific Expression of Foreign Gene in Postnatal Mouse Glial Cells using Electroporation

Ding

Hu²,

Zhao

et al. 2013

CNS Neurosci Ther

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Nonviral Gene Therapy of the Nervous System: Electroporation

Ding

Fan

2016

Gene Therapy for Neurological Disorders

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Electroporation has been widely used to efficiently transfer foreign genes into the mammalian central nervous system (CNS), and thus plays an important role in gene therapeutic studies on some brain disorders. A lot of work concerning electroporation is focused on gene transfer into rodent brains. This technique involves an injection of nucleic acids into the brain ventricle or specific area and then applying appropriate electrical field to the injected area. Here, we briefly introduced the advantages and the basic procedures of gene transfer into the rodent brain using electroporation. Better understanding of electroporation in rodent brain may further facilitate gene therapeutic studies on brain disorders.

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Gene Transfer into the Both Sides of Postnatal Cerebral Cortex Using Tweezer Electrode

Cited by 2 publications

References 7 publications

Specific Expression of Foreign Gene in Postnatal Mouse Glial Cells using Electroporation

Specific Expression of Foreign Gene in Postnatal Mouse Glial Cells using Electroporation

Nonviral Gene Therapy of the Nervous System: Electroporation

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