Novel natural genetic variation controlling the competence to form adventitious roots and shoots from the tomato wild relative Solanum pennellii

Arikita, Fernanda Namie; Azevedo, Mariana S.; Scotton, Danielle Camargo; Pinto, Maísa de Siqueira; Figueira, Antônio; Peres, Lázaro Eustáquio Pereira

doi:10.1016/j.plantsci.2012.11.005

Cited by 20 publications

(28 citation statements)

References 65 publications

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“…Considering the possibility that other wild species harbouring the allele r probably would harbor the genes related to the high organogenic capacity (PERES et al, 2001), in vitro organogenic capacity of the specie Solanum pennellii was tested, confirming that this specie also owns a high organogenic capacity (ARIKITA et al, 2013).…”

Section: Tomato In Vitro Regenerationmentioning

confidence: 91%

“…Hence, S. pennellii LA716, a green-fruited species, presented a high in vitro organogenesis capacity (ARIKITA et al, 2013). In addition, previous results have suggested the presence of other loci controlling in vitro regeneration in tomato (FARIA et al, 2002;KOORNNEEF et al, 1993).…”

Section: Introductionmentioning

confidence: 91%

“…In the last few years, studies involving these wild species, mainly S. peruvianum and S. pennellii allowed some aspects of tomato in vitro regeneration to be unraveled (ARIKITA et al, 2013;AZEVEDO, 2012;KOORNNEEF et al, 1987;LOMBARDI-CRESTANA et al, 2012;PINO et al, 2010) S. peruvianum high organogenic capacity is related mainly to two dominant alleles called REGENERATION 1 (Rg1) and REGENERATION 2 (Rg2) (KOORNNEEF et al, 1987).…”

Section: Tomato In Vitro Regenerationmentioning

confidence: 99%

“…Therefore, in order to identify key regulator of acquisition of competence, genes expressed a step before the commitment to organogenesis should be identified and characterized (SANTOS et al, 2009). Another approach is to search for and study genotypes (induced mutants and natural genetic variations) with high or low organ formation rates on both, SIM and RIM (ARIKITA et al, 2013;LOMBARDI-CRESTANA et al, 2012). Identifying these factors is an important step to further our understanding the organogenesis mechanisms, particularly the acquisition of competence phase (KOORNNEEF et al, 1993;LOMBARDI-CRESTANA et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…One of the ways to identify such genes is the characterization of those expressed in a previous step to the commitment to organogenesis (SANTOS et al, 2009), or alternatively the search for genotypes (induced mutants or natural genetic variation) with high or low organ formation in both Shoot-Inducing Medium (SIM) and Root-Inducing Medium (RIM) (ARIKITA et al, 2013;LOMBARDI-CRESTANA et al, 2012). The identification of such genes/molecules involved in the regeneration regulation would be an important step to understand the molecular events involved in the organogenic process, especially in the phase of acquisition of competence (KOORNNEEF et al, 1993;LOMBARDI-CRESTANA et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of natural genetic variations affecting tomato cell competence to assume different developmental fates

Pinto¹

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Characterization of natural genetic variations affecting tomato cell competence to assume different developmental fatesThe study of natural genetic variations affecting organogenic capacity in tomato (Solanum lycopersicum) is attractive due to the existence of several tomato wild relatives with enhanced organogenic capacity. The characterization of such variations is relevant not only in order to manipulate plant development, but also to understand its ecological and evolutionary significance. The objective of this work was to characterize three tomato loci whose alleles from the wild relative S. pennellii enhance in vitro shoot and root regeneration, and analyze their involvement in the acquisition of competence phase. In the first manuscript, we report the genetic and physiological characterization of the loci Rg3C, Rg7H and Rg8F. The S. pennellii alleles were introgressed into the tomato genetic model cv. Micro-Tom (MT), creating the near isogenic lines (NILs) MT-Rg3C, MT-Rg7H and MT-Rg8F. In the second manuscript we present a comparative analysis between the Near-Isogenic Lines (NILs) MT-Rg3C and MT-Rg1. Since Rg1 was proposed to be a key gene in the acquisition of competence, and was mapped in the chromosome three, it is believed that Rg3C is probably equivalent to the Rg1 allele from S. peruvianum. After the introgression of the loci into the MT background, the NILs presented enhanced regeneration of both roots and shoots, confirming that the loci were successfully introgressed. The analysis of the time for acquisition of competence and induction, together with the molecular characterization of the NILs, indicate that the genes present in the loci Rg3C, Rg7H and Rg8F affect in vitro regeneration by distinct pathways. While Rg3C decreased the time required for both acquisition of competence and induction, the other loci seem to influence only the time of acquisition of competence, in the case of Rg8F, or the time of induction, in the case of Rg7H. Additionally, although MT-Rg3C has an enhanced shoot branching phenotype, MT-Rg7H and MT-Rg8F did not differ from MT in this trait. This indicates that enhanced in vitro shoot formation in tomato is not necessarily related to a deleterious high branching phenotype. Comparative analyses of MT-Rg1 and MT-Rg3C strongly indicate that Rg1 and Rg3C are alleles of a same gene controlling regeneration capacity. Integrating Rg1 and Rg3C mapping information, we were able to narrow the number of candidate genes for Rg1/Rg3C to only 27, which were also analyzed and discussed.

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Section: Tomato In Vitro Regenerationmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 91%

Section: Tomato In Vitro Regenerationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of natural genetic variations affecting tomato cell competence to assume different developmental fates

Pinto¹

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Physionomics

Jesus

Zsögön

Peres

2014

Omics in Plant Breeding

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Natural Variation as a Tool to Investigate Nutrient Use Efficiency in Plants

Chietera

Chardon

2014

Plant Ecophysiology

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A huge natural variation exists between individuals within a given plant species. Most of the responses of growth-related traits to different environmental scenarios are genotype dependent. Hence, natural variation in plants provides an interesting and valuable source of genetic diversity to study plant responses to environmental factors. The identification of genes that underlie phenotypic variation has an enormous practical implication by providing a means to improve crop yield and quality. The approach based on natural variation aims to use naturally occurring differences to improve our knowledge about complex physiological responses of plants to their environment, including nutrition efficiency. An overview of different approaches currently used in plant research aimed at dissecting complex quantitative traits is presented here, with a special focus on those related to Nutrient Use Efficiency, to explain strategies based on QTL mapping in segregating populations and association mapping in wild populations. Some case studies regarding each of the investigative strategies described are detailed.

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Novel natural genetic variation controlling the competence to form adventitious roots and shoots from the tomato wild relative Solanum pennellii

Cited by 20 publications

References 65 publications

Characterization of natural genetic variations affecting tomato cell competence to assume different developmental fates

Characterization of natural genetic variations affecting tomato cell competence to assume different developmental fates

Physionomics

Natural Variation as a Tool to Investigate Nutrient Use Efficiency in Plants

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