Amounts and compositional analysis of glycosaminoglycans in the tissue of fish

Arima, Kazuya; Fujita, Hiroyuki; Toita, Ryosuke; Imazu-Okada, Ayaka; Tsutsumishita-Nakai, Nao; Takeda, Naoko; Nakao, Yasuhiro; Wang, Hui; Kawano, Manami; Matsushita, Kazuhiro; Tanaka, Haruna; Morimoto, Shin; Nakamura, Ayumi; Kitagaki, Masahiro; Hieda, Yuka; Hatto, Ryuya; Watanabe, Ayako; Yumura, Takeru; Okuhara, Takashi; Hayashi, Hiroki; Shimizu, Katsuhiko; Nakayama, Kiyoshi; Masuda, Shigeru; Ishihara, Yukio; Yoshioka, Shunsuke; Yoshioka, Shinobu; Shirade, Seizo; Tamura, Jun’ichi

doi:10.1016/j.carres.2012.11.010

Cited by 44 publications

(24 citation statements)

References 17 publications

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“…Based on the dehydrated tissue, a total GAGs extraction yield of approximately 0.1 ± 0.05% was revealed to be 10-fold lower than that of the overall extract (1%) from the skin tissue of the ray R. radula using same papain method (Mansour et al, 2009). This amount of GAGs contained in the O. niloticus skin contrasted with a previously described study from Arima et al (2013), who digested (55 °C, three h) defatted dry scale tissue with protease N Amano G, yielding a very scarce amount of total GAGs. This combined information allowed the assumption that some GAGs in this Cichlidae species varies depending on the tissue of origin.…”

Section: Tilapia Skin Reveals a Little Amount Of Matrix' Gagscontrasting

confidence: 78%

“…Total fraction yield was 3.66-fold greater compared with that found for this same fish species by Rodrigues et al (2011). On elution with stepwise of NaCl, the concentration of GAGs in O. niloticus skin was also greater than that of freshwater fish Cyprinus carpio (5.7 mg) (Rodrigues et al, 2009), but lower than those found in the deep-sea fishes Lycodes nakamurae and L. toyamensis (13 and 18 mg, respectively), when sulfated GAGs fractions were obtained by stepwise of LiCl (Arima et al, 2013).…”

Section: Combined Physical-chemical Analyses Reveal Unique Dsmentioning

confidence: 70%

“…GAGs comprise a variable family of highly complex glycans produced from vertebrate and invertebrate tissues (Volpi & Maccari, 2002;Arima et al, 2013;Pomin, 2014;Sayan et al, 2016). They are heteropolysaccharides consisting of repeated units of alternating residues of N-acetylgalactosamine or glycosamine linked to a non-nitrogenated sugar, like uronic acid (D-glycuronic acid or L-iduronic acid) or a galactose, making themselves typical chains are known as mucopolysaccharides, and their association to proteoglycans form the matrix' principal elements playing several physiological and pathological events (Tovar et al, 2005;Volpi, 2011;Oliveira et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…GAGs have been traditionally used as health food ingredients and medications (Volpi, 2011); however, the decontrolled fishing of sharks (Arima et al, 2013) and lobsters (Sayan et al, 2016) and the risk of viral contamination from the pig as another source of commercially-important GAGs (Mourão, 2015), as well as the price and limited supply of GAGs produced by fermentation from bacteria or extracted from avian (Arima et al, 2013) indicate the need to look for alternative sources of glycans with both economic and environmental benefits (Cardozo et al, 2007;Volpi, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…GAGs from fish tissues have been isolated as a highly valuable platform for the valorization of residues obtained from food processing for the development of novel commerciallyimportant biomaterials (Arima et al, 2013). DS has been predominantly found in the skin of several marine fish species than other GAGs types (Dellias et al, 2004;Souza et al, 2007;Mansour et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of thrombin generation by dermatan sulfate isolated from the skin of Oreochromis niloticus

Salles

Rodrigues

Barcellos

et al. 2017

Agraria

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Oreochromis niloticus has skin anticoagulant glycosaminoglycans (GAGs), but their effects on thrombin generation (TG) are unknown. This study partially characterized skin GAGs and analyzed as inhibitors of TG. Papain-extraction yield of 0.1% contained two fractions separated by DEAE-cellulose chromatography, differing on charge density and carboxylated groups by a combination of agarose/polyacrylamide electrophoresis and sequential toluidine blue/stains-all staining, presenting molecular sizes ca. 40 kDa. Depolymerization of the fractions with chondroitin ABC lyase showed dermatan sulfate (DS) as the unique GAG by agarose analysis. Both activated partial thromboplastin time (APTT) and prothrombin time tests only showed anticoagulation by fractions and mammalian DS by APTT (0.61, 0.47 and 1.72 IU, respectively) against heparin (193 IU). Fractions acted concentrationdependent on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with more than 50% inactivation (41.6 and 83.3 µg), whereas DS and heparin entirely abolished at low amounts. DS from O. niloticus skin blocks in vitro TG in human plasma.Key words: Cichlidae; freshwater fish; sulfated glycans; thrombosis in vitro; waster Inibição da geração de trombina por dermatam sulfato isolado da pele de Oreochromis niloticus RESUMOOreochromis niloticus possui glicosaminoglicanos (GAGs) anticoagulantes de pele, porém desconhecidos são seus efeitos sobre geração de trombina (GT). Caracterizou-se parcialmente GAGs de pele e analisou-se como inibidores de GT. Rendimento de 0,1% da extração com papaína, conteve duas frações separadas por cromatografia de DEAE-celulose, e por combinação de eletroforese de agarose/poliacrilamida e coramento sequencial azul de toluidina/"stains-all", diferiram quanto à densidade da carga e grupos carboxilados, apresentando tamanhos moleculares ca. 40 kDa. Depolimerização das frações com condroitinase ABC mostrou, por análise em agarose, dermatam sulfato (DS) como GAG único. Ambos os testes do tempo de tromboplastina parcial ativada (TTPA) e do tempo de protrombina mostraram anticoagulação, das frações e DS de mamífero, somente pelo TTPA (0,61; 0,47 e 1,72 UI, respectivamente) contra ao da heparina (193 UI). Frações atuaram sobre ambas vias intrínsica/ extrínsica dependente de concentração na GT usando plasma humano diluído 60 vezes, com inativação mais que 50% (41,6 e 83,3 µg), enquanto em quantidades baixas DS e heparina aboliram totalmente. DS da pele de O. niloticus bloqueia GT in vitro no plasma humano. Palavras-chave:Cichlidae; peixe dulcícola; glicanos sulfatados; trombose in vitro; resíduo

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Section: Tilapia Skin Reveals a Little Amount Of Matrix' Gagscontrasting

confidence: 78%

Section: Combined Physical-chemical Analyses Reveal Unique Dsmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Inhibition of thrombin generation by dermatan sulfate isolated from the skin of Oreochromis niloticus

Salles

Rodrigues

Barcellos

et al. 2017

Agraria

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Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering

Khajavi

Hajimoradloo

Zandi

et al. 2021

J Biomedical Materials Res

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Here, engineered cartilage‐like scaffold using an extracellular matrix (ECM) from sturgeon fish cartilage provided a chondroinductive environment to stimulate cartilaginous matrix synthesis in human adipose stem cells (hASCs). Three dimensional porous and degradable fish cartilage ECM‐derived scaffold (FCS) was produced using a protocol containing chemical decellularization, enzymatic solubilization, freeze‐drying and EDC‐crosslinking treatments and the effect of different ECM concentrations (10, 20, 30, and 40 mg/ml) on prepared scaffolds was investigated through physical, mechanical and biological analysis. The histological and scanning electron microscopy analysis revealed the elimination of the cell fragments and a 3‐D interconnected porous structure, respectively. Cell viability assay displayed no cytotoxic effects. The prepared porous constructs of fish cartilage ECM were seeded with hASCs for 21 days and compared to collagen (Col) and collagen‐10% hyaluronic acid (Col‐HA) scaffolds. Cell culture results evidenced that the fabricated scaffolds could provide a proper 3‐D structure to support the adhesion, proliferation and chondrogenic differentiation of hASCs considering the synthesis of specific proteins of cartilage, collagen type II (Col II) and aggrecan (ACAN). Based on the results of the present study, it can be concluded that the porous scaffold derived from fish cartilage ECM possesses an excellent potential for cartilage tissue engineering.

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Identification and quantification of uronic acid-containing polysaccharides in tissues of Russian sturgeon (Acipenser gueldenstaedtii) by HPLC–MS/MS and HPLC–MSn

Guo

Wang

Liu

et al. 2016

Eur Food Res Technol

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Amounts and compositional analysis of glycosaminoglycans in the tissue of fish

Cited by 44 publications

References 17 publications

Inhibition of thrombin generation by dermatan sulfate isolated from the skin of Oreochromis niloticus

Inhibition of thrombin generation by dermatan sulfate isolated from the skin of Oreochromis niloticus

Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering

Identification and quantification of uronic acid-containing polysaccharides in tissues of Russian sturgeon (Acipenser gueldenstaedtii) by HPLC–MS/MS and HPLC–MSn

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