Identification and quantification of uronic acid-containing polysaccharides in tissues of Russian sturgeon (Acipenser gueldenstaedtii) by HPLC–MS/MS and HPLC–MSn

Guo, Li; Wang, Peng; Liu, Bin; Ai, Chunqing; Zhou, Da‐Yong; Song, Shuang; Zhu, Beiwei

doi:10.1007/s00217-016-2834-6

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…The preferred extraction solvent for phenolic compounds in plant materials is methanol, 35–39 but the extraction conditions applied vary widely. According to the literature, 21,25,26,40,41 the most significant factors affecting the extraction of flavonoids were selected: extraction temperature (50–80°C), extraction time (15–60 min), methanol concentration (50–100%, v/v ). A two‐level three‐factor full factorial design of experiment was used to find the optimum extraction conditions.…”

Section: Resultsmentioning

confidence: 99%

“…The preferred extraction solvent for phenolic compounds in plant materials is methanol, [35][36][37][38][39] but the extraction conditions applied vary widely. According to the literature, 21,25,26,40,41 At optimised conditions, the characteristic profiles of flavonoids of aeroponic I. sibirica L. and biotechnological raw plant materials, grown on nutrient media with phytohormonal additives of 6-BAP and NAA in various concentrations were obtained by HPLC-MS/MS timsTOF in negative and positive ionisation mode (Figure 2). Plants grown on 1 μM 6-BAP nutrient media were used as a control.…”

Section: Procedures For the Optimisation Of Extraction Of Flavonoids ...mentioning

confidence: 99%

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Development of approach for flavonoid profiling of biotechnological raw materials Iris sibirica L. by HPLC with high‐resolution tandem mass spectrometry

Karpitskiy

Бессонова

Карцова

et al. 2022

Phytochemical Analysis

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Introduction Iris L. are promising in medicine due to the biological activity of extracts. Iris sibirica L. is spread in Russia but its phytochemical composition has not been studied in detail though it is included in the Red Book. For this reason, I. sibirica L. biotechnology is in high demand. One of the key points in biotechnology is the regulation of plant metabolism using phytohormones. Obtaining of chromatographic metabolite profiles allows to control this process. Objective The aim of this study was to develop an approach for effective control of biotechnological raw materials of I. sibirica L. by flavonoid profiles using high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) and to investigate the influence of phytohormones in nutrient media on content of flavonoids. Methodology Iris sibirica L. regenerated plants were grown on Murashige–Skoog media with 6‐benzylaminopurine (6‐BAP) and α‐naphtylacetic acid (NAA) additives. To optimise extraction conditions, the design of the experiment was used. Profiles of polyphenols were obtained by HPLC–MS/MS in the positive and negative ionisation modes. Results The process for efficient extraction from leaves of I. sibirica L. were developed. The factors influencing the extraction efficiency of flavonoids have been determined. A total of 36 compounds were identified by HPLC–MS/MS. Among them isoflavones and their glycosides are the main classes. Addition of an auxin‐like hormone increased the non‐polar flavonoid levels, but decreased the polar ones. The variation in concentration of cytokinin (6‐BAP) affected almost all of the analytes. Conclusion The methodology for effective control of I. sibirica L. raw plant material biotechnology was developed by analysing obtained chromatographic polyphenol profiles.

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Section: Resultsmentioning

confidence: 99%

Section: Procedures For the Optimisation Of Extraction Of Flavonoids ...mentioning

confidence: 99%

Development of approach for flavonoid profiling of biotechnological raw materials Iris sibirica L. by HPLC with high‐resolution tandem mass spectrometry

Karpitskiy

Бессонова

Карцова

et al. 2022

Phytochemical Analysis

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“…The results showed that the polysaccharide extracted via pH 7.5, 50 • C enzymolysis for 4 h followed by 400 Watt ultrasonic for 50 min had a higher sulfate acid group content, and the yield was better than the other two methods, about 6.09% [27]. Guo et al conducted a study on the extraction of polysaccharides from various parts of Scophthalmus maximus using two methods: first, alkaline followed by enzyme, and second, enzyme followed by alkaline [28]. The results show that enzymes are used to hydrolyze the peptide bond first, which degrades the protein into small peptides and thus separates it from other substances.…”

Section: Combined Extraction Methodsmentioning

confidence: 99%

The Relationship between Preparation and Biological Activities of Animal-Derived Polysaccharides: A Comprehensive Review

Yang,

Liu

et al. 2024

Foods

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Polysaccharides are biomolecules found in microorganisms, plants, and animals that constitute living organisms. Glycosaminoglycans, unique acidic polysaccharides in animal connective tissue, are often combined with proteins in the form of covalent bonds due to their potent biological activity, low toxicity, and minimal side effects, which have the potential to be utilized as nutrition healthcare and dietary supplements. Existing studies have demonstrated that the bioactivity of polysaccharides is closely dependent on their structure and chain conformation. The characteristic functional groups and primary structure directly determine the strength of activity. However, the relationship between structure and function is still unclear, and the target and mechanism of action are not fully understood, resulting in limited clinical applications. As a result, the clinical applications of these polysaccharides are currently limited. This review provides a comprehensive summary of the extraction methods, structures, and biological activities of animal-derived polysaccharides that have been discovered so far. The aim is to promote developments in animal active polysaccharide science and provide theoretical support for exploring other unknown natural products.

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“…Sturgeon is one of the most ancient bony fishes, the class Osteichthyes (Guo et al, 2017), and one among the most important cold-water fishes farmed commercially (Hao et al, 2015;Gui et al, 2014). In 2017, the output of Chinese sturgeon breeding reached 83,058 tons, an increase of 5.45% from 2016 (Xu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Application of an artificial neural network model to predict the change of moisture during drying of sturgeon bone marrow

Jiang

Shang

Zheng

et al. 2023

Qual. Assur. Saf. Crops Foods

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In the experiment of this article, the artificial neural network (ANN) was used to establish the sturgeon bone marrow drying model. Further, the effects of different temperatures (40, 60, and 80°C), humidities (0, 20, and 40%), and air velocities (8, 16, and 25 m/s) on the drying characteristics of sturgeon bone marrow were stud-ied. The studies had shown that with the increase of drying temperature, the acceleration of air velocity, and the decrease of humidity, the sturgeon bone marrow can be dried in the shortest period of 100 min. This study used ANN to feasibly predict dried sturgeon bone marrow moisture ratio, based on the time, temperature, humidity, and air velocity drying inputs. The results revealed that 11 hidden neurons were selected as the best configuration to predict the moisture ratio. This network was able to predict moisture ratio with R value 0.996. This model correctly predicted the optimal drying conditions and established that temperature is the single most significant factor in determining the drying time of sturgeon bone marrow. It is expected that this system will have broader application in other food drying requirements.

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Identification and quantification of uronic acid-containing polysaccharides in tissues of Russian sturgeon (Acipenser gueldenstaedtii) by HPLC–MS/MS and HPLC–MSn

Cited by 8 publications

References 23 publications

Development of approach for flavonoid profiling of biotechnological raw materials Iris sibirica L. by HPLC with high‐resolution tandem mass spectrometry

Development of approach for flavonoid profiling of biotechnological raw materials Iris sibirica L. by HPLC with high‐resolution tandem mass spectrometry

The Relationship between Preparation and Biological Activities of Animal-Derived Polysaccharides: A Comprehensive Review

Application of an artificial neural network model to predict the change of moisture during drying of sturgeon bone marrow

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