Heavy metal resistant strains are widespread along Streptomyces phylogeny

Álvarez, Analía; Catalano, Santiago A.; Amoroso, Maria Julia del R.

doi:10.1016/j.ympev.2012.11.025

Cited by 25 publications

(14 citation statements)

References 32 publications

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“… Abbes and Edwerds (1990) evaluated the toxicity of various PTE including Cd, Co, Cu, Cr, Hg, Ni, Zn, and Mn on 34 Streptomyces species representative of various taxonomic clusters. Another study described the isolation of several Streptomyces strains with resistances to different PTE from contaminated areas, and some exhibited multiple tolerances against different PTE ( Álvarez et al, 2013 ). Due to the extreme abundance of the genus Streptomyces in our study, the further characterization of tolerant Streptomyces from this red gypsum dump might lead to a better assessment/new discovery of the physiological mechanisms involved in the metal tolerance in this genus and therefore to ecological applications.…”

Section: Resultsmentioning

confidence: 99%

Streptomyces Dominate the Soil Under Betula Trees That Have Naturally Colonized a Red Gypsum Landfill

et al. 2018

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The successful restoration of well-engineered tailings storage facilities is needed to avoid mine tailings problems. This study characterized the bacterial communities from vegetated and non-vegetated soils from a red gypsum landfill resulting from the industrial extraction of titanium. A set of 275 bacteria was isolated from vegetated soil and non-vegetated soil areas and taxonomically characterized using BOX-PCR. The study also evaluated the ability of a subset of 88 isolated bacteria on their ability to produce plant growth promoting (PGP) traits [indoleacetic acid (IAA) production, phosphate solubilization, and siderophore production] and their tolerance to potentially toxic elements (PTEs). Twenty strains were chosen for further analysis to produce inoculum for birch-challenging experiments. Principal component analysis (PCA) showed that the set of pedological parameters (pH, granulometry, carbon, organic matter, and Mg content) alone explained approximately 40% of the differences between the two soils. The highest density of total culturable bacteria was found in the vegetated soil, and it was much higher than that in the non-vegetated soil. The Actinobacteria phyla dominated the culturable soil community (70% in vegetated soil and 95% in non-vegetated soil), while the phyla Firmicutes (including the genus Bacillus) and Bacteroides (including the genera Pedobacter and Olivibacter) were found only in the vegetated soil fraction. Additional genera (Rhizobium, Variovorax, and Ensifer) were found solely in the vegetated soil. The vegetated soil bacteria harbored the most beneficial PGP bacteria with 12% of the isolates showing three or more PGP traits. The strains with higher metal tolerances in our study were Phyllobacterium sp. WR140 (RO1.15), Phyllobacterium sp. WR140 (R01.34), and Streptomyces sp. (R04.15), all isolated from the vegetated soil. Among the isolates tested in challenging experiments, Phyllobacterium (R01.34) and Streptomyces sp. (R05.33) have the greatest potential to act as PGP rhizobacteria and therefore to be used in the biological restoration of tailings dumps.

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Section: Resultsmentioning

confidence: 99%

Streptomyces Dominate the Soil Under Betula Trees That Have Naturally Colonized a Red Gypsum Landfill

et al. 2018

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“…In fact, the most abundant Actinobacteria genera ( Streptomyces, Frankia, Mycobacterium ), which varied between polluted and unpolluted lakes, fall in the same abundance cluster (see Actinobacteria in Supplementary File S4 ). Indeed, some Actinobacteria (e.g., Streptomyces ) strains are known to have different metal-resistance profiles (Álvarez et al, 2013 ). Interestingly, strains like Mycobacterium were able to transport and uptake Cd (Dimkpa et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Taxon-Function Decoupling as an Adaptive Signature of Lake Microbial Metacommunities Under a Chronic Polymetallic Pollution Gradient

et al. 2018

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Adaptation of microbial communities to anthropogenic stressors can lead to reductions in microbial diversity and disequilibrium of ecosystem services. Such adaptation can change the molecular signatures of communities with differences in taxonomic and functional composition. Understanding the relationship between taxonomic and functional variation remains a critical issue in microbial ecology. Here, we assessed the taxonomic and functional diversity of a lake metacommunity system along a polymetallic pollution gradient caused by 60 years of chronic exposure to acid mine drainage (AMD). Our results highlight three adaptive signatures. First, a signature of taxon—function decoupling was detected in the microbial communities of moderately and highly polluted lakes. Second, parallel shifts in taxonomic composition occurred between polluted and unpolluted lakes. Third, variation in the abundance of functional modules suggested a gradual deterioration of ecosystem services (i.e., photosynthesis) and secondary metabolism in highly polluted lakes. Overall, changes in the abundance of taxa, function, and more importantly the polymetallic resistance genes such as copA, copB, czcA, cadR, cCusA, were correlated with trace metal content (mainly Cadmium) and acidity. Our findings highlight the impact of polymetallic pollution gradient at the lowest trophic levels.

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“…Members of Streptomyces and Amycolatopsis genera can survive in polluted environments with heavy metals as mentioned by Polti et al [ 16 ] and Albarracín et al [ 17 ]. Álvarez et al [ 63 ] reported that the presence of heavy metal resistant strains in different Streptomyces clades may have two different explanations: (i) the resistance was already present in the most recent common ancestor (MRCA) and was then inherited by the different lineages; (ii) the different lineages inherited from the MRCA the capacity to develop new mechanisms or modify existing ones, in order to generate resistance to heavy metals. Schütze and Kothe [ 64 ] indicated that several morphological, physiological, and reproductive characteristics of Streptomyces (the filamentous growth, the formation of hyphae, and the production of spores) would allow its species to occupy extreme environments.…”

Section: Discussionmentioning

confidence: 99%

Resistance to and Accumulation of Heavy Metals by Actinobacteria Isolated from Abandoned Mining Areas

Baz

Barakate

et al. 2015

The Scientific World Journal

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Accumulation of high concentrations of heavy metals in environments can cause many human health risks and serious ecological problems. Nowadays, bioremediation using microorganisms is receiving much attention due to their good performance. The aim of this work is to investigate heavy metals resistance and bioaccumulation potential of actinobacteria strains isolated from some abandoned mining areas. Analysis of mining residues revealed that high concentration of zinc “Zn” was recorded in Sidi Bouatman, Arbar, and Bir Nhass mining residues. The highest concentration of lead “Pb” was found in Sidi Bouatman. Copper “Cu,” cadmium “Cd,” and chromium “Cr” were found with moderate and low concentrations. The resistance of 59 isolated actinobacteria to the five heavy metals was also determined. Using molecular identification 16S rRNA, these 27 isolates were found to belong to Streptomyces and Amycolatopsis genera. The results showed different levels of heavy metal resistance; the minimum inhibitory concentration (MIC) recorded was 0.55 for Pb, 0.15 for Cr, and 0.10 mg·mL−1 for both Zn and Cu. Chemical precipitation assay of heavy metals using hydrogen sulfide technic (H2S) revealed that only 27 isolates have a strong ability to accumulate Pb (up to 600 mg of Pb per g of biomass for Streptomyces sp. BN3).

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Heavy metal resistant strains are widespread along Streptomyces phylogeny

Cited by 25 publications

References 32 publications

Streptomyces Dominate the Soil Under Betula Trees That Have Naturally Colonized a Red Gypsum Landfill

Streptomyces Dominate the Soil Under Betula Trees That Have Naturally Colonized a Red Gypsum Landfill

Taxon-Function Decoupling as an Adaptive Signature of Lake Microbial Metacommunities Under a Chronic Polymetallic Pollution Gradient

Resistance to and Accumulation of Heavy Metals by Actinobacteria Isolated from Abandoned Mining Areas

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