Effect of the addition of IGF-I and vitamin E to stored boar semen

Mendez, M. F. B.; Zangerônimo, Márcio Gilberto; Rocha, Luiz Célio Souza; Faria, Bruno Generoso; Pereira, Bárbara Azevedo; Fernandes, Carolina Dias; Chaves, Bruna Resende; Murgas, Luís David Solis; Sousa, Raimundo Vicente de

doi:10.1017/s1751731112002285

Cited by 23 publications

(23 citation statements)

References 20 publications

(28 reference statements)

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“…The possible mechanism of how IGF-I maintains motility and MMP is assumed to be through energy metabolism [1], antioxidant effects [13] and high intracellular calcium level by increased ion transport [2]. In contrast, activation of cellular metabolism by IGF-I may also be related to the generation of free radicals [6]. In our study, the 100 and 200 n g/m l IGF-I concentrations were optimal level and had a positive effect without toxicity to canine spermatozoa.…”

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confidence: 99%

IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa

Shin

Kim

Hong

et al. 2014

The Journal of Veterinary Medical Science

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The aim of study was to evaluate the effects of insulin-like growth factor I (IGF-I) on canine sperm function during cooled and freeze-thaw storage. Extenders supplemented with different IGF-I concentrations (0, 100 and 200 ng/ml) were added to canine spermatozoa, and the sperm samples were stored at 4°C for 48 hr or freeze-thawed. Sperm motility, morphology, plasma-membrane integrity (PMI) and mitochondrial membrane potential (MMP) were evaluated. IGF-I had no effect on PMI or morphology during cooling and freeze-thawing. However, IGF-I alleviated the reduction in progressive motility and MMP caused by cooled storage and led to an improvement in MMP after freeze-thawing. In conclusion, IGF-I can be helpful to maintain progressive motility of canine spermatozoa during hypothermic storage via increased MMP.

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IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa

Shin

Kim

Hong

et al. 2014

The Journal of Veterinary Medical Science

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“…O acréscimo de α-Tocoferol na concentração 200 μg/mL em diluidor na tentativa de melhorar a viabilidade do sêmen suíno submetido à refrigeração, não se mostrou vantajoso neste estudo, independente da temperatura utilizada, corroborando com Mendez et al (2013), que avaliaram diferentes concentrações de α-tocoferol (100, 200, 400 μg/mL) e também não observaram nenhuma ação sobre a motilidade espermática, entre essas concentrações ao longo de 72 horas de refrigeração a 15 °C.…”

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“…Da mesma forma, Althouse et al (1998) superior a 75% nas amostras preservadas entre 12 e 17 °C, durante 48 horas de refrigeração, e motilidade inferior a 70% nas amostras mantidas entre 8 e 10 °C, sugerindo que a temperatura crítica para resfriamento do sêmen suíno encontra-se a abaixo de 12 °C. Esses resultados corroboram com Mendez et al (2013), que também não observaram nenhum efeito sobre o vigor dos espermatozoides, ao utilizar α-Tocoferol no sêmen refrigerado a 15 °C ao longo de 72 horas, e também com Satorre et al (2012) que observaram que não houve diferença significativa (p>0,05) para quantidade de espermatozoides com membrana íntegra de amostras de sêmen congelados em diluidor acrescido ou não de α-Tocoferol, o que é contraditório uma vez que se esperava uma redução do estresse oxidativo resultando na melhor preservação de membranas com o uso dessa substância. Talvez haja necessidade de se testar outras concentrações de α-Tocoferol assim como avaliar maiores tempos de incubação do sêmen nos diluidores com antioxidante.…”

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Efeito da Vitamina E (±-Tocoferol) e de Diferentes Temperaturas na Viabilidade de Sêmen Suíno Refrigerado.

Labêta¹,

Mello²,

Silenciato³

et al. 2017

Braz. J. Vet. Med.

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Copyright Labêta et al. Este é um artigo publicado em acesso aberto (Open Access) sob a licença Creative Commons Attribution Non-Commercial, que permite uso, distribuição e reprodução em qualquer meio, sem restrições desde que sem fins comerciais e que o trabalho original seja corretamente citado. ) com nível de significância de 5%. Houve diferença significativa (p<0,05) para a motilidade, quando foram comparadas as temperaturas de 5 e 15 °C independentemente do uso do α-Tocoferol durante o período entre 12 e 72 horas. Os valores mais altos de motilidade foram obtidos com a temperatura de 15 °C. Observou-se também diferença (p<0,05) para a motilidade entre temperaturas de 5 e 15 °C quando comparado apenas com as amostras acrescidas de α-Tocoferol, bem como as amostras sem esta substância. A maior motilidade foi obtida novamente com a temperatura de 15 °C. Não foi observada diferença no vigor e na integridade da membrana (teste hiposmótico) entre as amostras adicionadas ou não de α-Tocoferol, em qualquer uma das temperaturas e tempos estudados. Conclui-se que a temperatura de 15 °C foi mais eficiente para a conservação de sêmen de cachaços durante 72 horas. Conclui-se também que a adição de 200 μg/mL de α-Tocoferol não melhorou a viabilidade dos espermatozoides submetidos à refrigeração. Efeito da vitamina E (α-TocoferolPalavras-chave: ejaculado, refrigeração, cachaço. AbstractThe objective of this study was to evaluate the addition of α-Tocopherol to swine insemination doses stored at 5 and 15 °C for a period of 72 hours. Semen samples (n=9) collected from three adult Pietrain boars were treated with or without 200 μg/mL of α-Tocopherol. The treatments were: T1: α-Tocopherol + cooling to 5 °C; T2: α-Tocopherol + cooling to 15 °C; T3: no α-Tocopherol + cooling to 5 °C; T4: no α-Tocopherol + cooling to 15 °C. All samples were evaluated up to 72 hours for sperm motility and intensity of movements every 12 hours, and the hiposmotic test was performed every 24 hours. The average of the motility, the intensity of movements and hiposmotic test (HOST) were analyzed by chi-square test (х 2 ) with 5% significance level. There was a significant difference (p <0.05) for motility when comparing the temperatures of 5 and 15 °C regardless of α-Tocopherol during the period between 12 and 72 hours. The highest motility values were obtained at 15 °C. It was also observed a difference (p <0.05) for sperm motility between temperatures of 5 to 15 °C when compared only the samples with α-Tocopherol, as well as the samples without this substance. The higher motility was obtained with 15 °C. No difference was observed for the intensity of movements and membrane integrity between samples added or not of α-Tocopherol, in any of the temperatures and times evaluated. We concluded that the temperature of 15 °C was more efficient for boar semen preservation over 72 hours. It is also concluded that the addition of 200 μg/mL of α-Tocopherol showed no improvement in the viability of cooled porcine semen.

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“…After that time, there is a considerable reduction in quality, mainly due to a reactive oxygen species-induced (ROS-induced) overall cell membrane alteration (Ball et al, 2001;Breininger et al, 2005). Because of this, different metabolite substances have frequently been added to semen extenders in order to counteract the deleterious effects caused by inappropriate functioning of the endogenous boar sperm metabolism (Mendez et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Semen quality and reproductive performance of boars kept in pens containing conventional coffee husk as a floor covering

et al. 2016

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-The objective of this study was to evaluate the putative effects of the use of coffee husk as floor covering on boar semen quality. Sixteen boars were divided into two groups. The first group was held in a conventional system with a compact floor. The second group was kept on a compact floor covered with coffee pods. The experimental period was 60 days. Levels of caffeine and chlorogenic acid in the insemination doses (ID) before and after 96 h of storage at 15 °C and the reproductive performance (litter size and rate of return to estrus) of these doses after their utilization in a standard artificial insemination protocol were evaluated. The ID from the animals kept in pens containing the coffee pods contained 25.4±8.7 µg/mL of caffeine, whereas no significant amounts of chlorogenic acid were detected. Semen from boars housed with coffee husk showed a significant increase in malondialdehyde levels, which indicates a significant increase in cell membrane peroxidation, after 96 h of storage. There were no significant differences in the other evaluated semen quality parameters when analyzed among groups. Likewise, there was no significant influence of coffee hulls on either the rate of return to estrus or the litter size of inseminated females. The use of coffee husks as floor covering worsens the quality of fresh semen and that of insemination doses stored for 96 h but not the quality of semen immediately diluted; therefore, coffee husks may be used only on farms that use semen immediately processed after collection without affecting the reproductive parameters of breeding stock.

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Effect of the addition of IGF-I and vitamin E to stored boar semen

Cited by 23 publications

References 20 publications

IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa

IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa

Efeito da Vitamina E (±-Tocoferol) e de Diferentes Temperaturas na Viabilidade de Sêmen Suíno Refrigerado.

Semen quality and reproductive performance of boars kept in pens containing conventional coffee husk as a floor covering

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