IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of          Canine Spermatozoa

Shin, Sang‐Ouk; Kim, Suhee; Hong, Jin-Gi; Kim, Yong-Jun

doi:10.1292/jvms.14-0049

Cited by 14 publications

(10 citation statements)

References 14 publications

(19 reference statements)

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“…It is possible to improve the stallion semen quality by addition of insulin-like growth factor-I (IGF-I) to extenders (CHAMPION et al, 2002). IGF-I is a protein hormone that helps glucose (HERNANDEZ-GRAZÓN et al, 2016) and other energy sources as fructose (SELVARAJU et al, 2009), lactate and oxygen to enter the cell (TRAVASCIO et al, 2014), resulting in improved mitochondrial membrane potential during hypothermic storage of semen (SHIN et al, 2014). Also, there is a relevant function for IGF-I in β-oxidation and cholesterol synthesis and this growth factor has an important antioxidant activity (DE ITA et al, 2015) by stimulation of glutathione peroxidase.…”

Section: Introductionmentioning

confidence: 99%

“…Also, there is a relevant function for IGF-I in β-oxidation and cholesterol synthesis and this growth factor has an important antioxidant activity (DE ITA et al, 2015) by stimulation of glutathione peroxidase. In general, IGF-I has been shown to improve the quality of bovine (HENRICKS et al, 1998), bubaline (SELVARAJU et al, 2016, ovine (MAKAREVICH et al, 2012;PADILHA et al, 2012), swine (SILVA et al, 2011 and canine (SHIN et al, 2014) spermatozoa. In addition, post-thawed supplementation of IGF-I has improved semen quality in buffalo spermatozoa (SELVARAJU et al, 2009;SELVARAJU et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Silva

Holden²,

Souza³

et al. 2019

R. Agrogeoambiental

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High seminal quality is important to achieve acceptable pregnancy rate following artificial insemination with cryopreserved semen in the equine industry. Docosahexaenoic acid (DHA) is an omega-3-polyunsaturated acid, which improves the integrity of the spermatozoa membrane during temperature changes. Insulin-like growth factor-I (IGF-I) is a protein hormone that helps mainly glucose to enter spermatozoa and it is an antioxidant. The aim of this study was to assess the effect of the in vitro addition of DHA in combination with IGF-I to frozen-thawed stallion semen. Three ejaculates from each of three Irish Sport Horse stallions were collected, the gel fraction was removed and semen was diluted in a 1:1 ratio using extender, centrifuged (1.000 g) for 10 minutes at 32 °C and ressuspended to 100 x 10 6 spermatozoa/mL in freezing extender. Semen was cooled to 4 °C, packed into 0.5 mL straws, frozen and stored under liquid nitrogen at-196 °C. Straws were thawed at 37 °C for 30 seconds, semen was diluted to 25 x 10 6 spermatozoa/mL and split in four treatments adding 0 or 1 ng of DHA /mL and 0 or 100 ng of IGF-I /mL: DHA0, DHA0 + IGF-I, DHA1 and DHA1 + IGF-I. Semen was incubated at 32 °C and after 30 minutes, total motility (TM), rapid progressive motility (PM), viability and acrosome integrity were assessed. After 60 and 120 minutes, TM and PM were assessed again. Post-thawed PM was higher (P < 0.05) when DHA1 + IGF-I was added, but there was no effect of the addition of DHA and IGF-I to TM, viability or acrosome integrity (P > 0.05).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Silva

Holden²,

Souza³

et al. 2019

R. Agrogeoambiental

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“…In particular, insulinlike growth factor 1 (IGF-1) improves the quality of mammalian spermatozoa [9][10][11]. Insulinlike growth factor 1 is a singlechain mitogenic protein that is mostly found in highly proliferative cells and has been reported to be an important factor for germ cell development, maturation, capacitation, and spermatozoa motility [10].…”

Section: Introductionmentioning

confidence: 99%

Insulinlike growth factor I improves yak (Bos grunniens) spermatozoa motility and the oocyte cleavage rate by modulating the expression of Bax and Bcl-2

et al. 2015

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“…More suitable energy sources for frozen-thawed sperm have also been examined [ 16 , 76 ]. In addition, the effects of spermine [ 77 ], kinetin [ 78 ], cholesterol-loaded cyclodextrin [ 79 ], iodixanol [ 80 ], metformin [ 81 ], stem cells [ 82 ], stem cell-conditioned medium [ 83 ], norepinephrine metabolite [ 84 ], and insulin-like growth factor-1 [ 85 ] have been examined. The effects of each treatment on the in vitro motility of frozen-thawed spermatozoa have been documented.…”

Section: Extender For Canine Spermatozoamentioning

confidence: 99%

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

2022

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Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. Their widespread use has contributed to improvements in human health and welfare. However, in dogs, only artificial insemination using frozen semen is readily available as an ART to improve breeding and control genetic diversity. A recent priority in sperm cryopreservation is the development of alternatives to egg yolk, which is widely used as a component of the sperm extender. Egg yolk can vary in composition among batches and is prone to contamination by animal pathogens. The latter can be a problem for international exchange of cryopreserved semen. Low-density lipoprotein and skim milk are promising candidates for use as extenders, to ensure fertility after artificial insemination. Although not tested for its effects on fertility following artificial insemination, polyvinyl alcohol may also be a useful alternative to egg yolk as an extender. The development of cryopreservation techniques for canine embryos lags behind that for other mammals, including humans. However, given the success of non-surgical embryo transfer in 2011, studies have sought to refine this approach for practical use. Research on sperm cryopreservation has yielded satisfactory results. However, investigation of other approaches, such as cryopreservation of oocytes and gonadal tissues, remains insufficient. Techniques for the efficient induction of estrus may aid in the development of successful canine ARTs.

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IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa

Cited by 14 publications

References 14 publications

In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Insulinlike growth factor I improves yak (Bos grunniens) spermatozoa motility and the oocyte cleavage rate by modulating the expression of Bax and Bcl-2

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

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