Analysis of koi herpesvirus latency in wild common carp and ornamental koi in Oregon, USA

Xu, Jin; Bently, Jennifer; Beck, Linda; Reed, Aimee N.; Miller-Morgan, Tim; Heidel, Jerry R.; Kent, Michael L.; Rockey, Daniel D.; Jin, Ling

doi:10.1016/j.jviromet.2012.11.015

Cited by 39 publications

(29 citation statements)

References 17 publications

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“…First, koi carp with previous exposure to the virus displayed CyHV-3 DNA in white blood cells in the absence of any clinical signs or detectable infectious viral particles . Similar results were found in wild carp collected from ponds in Oregon with no history of CyHV-3 outbreaks (Xu, Bently, et al, 2013). Interestingly, Eide, Miller-Morgan, Heidel, Kent, et al (2011) detected low amounts of CyHV-3 DNA ranging from 2 to 60 copies per μg of isolated DNA in white blood cells of previously infected koi.…”

Section: Latent Infectionsupporting

confidence: 76%

“…In another report, a netting stress induced viral reactivation without symptoms 81 days after initial infection as detected by qPCR on gill samples (Bergmann & Kempter, 2011). Recent studies suggested that white blood cells could support CyHV-3 latency Eide, MillerMorgan, Heidel, Kent, et al, 2011;Reed et al, 2014;Xu, Bently, et al, 2013). First, koi carp with previous exposure to the virus displayed CyHV-3 DNA in white blood cells in the absence of any clinical signs or detectable infectious viral particles .…”

Section: Latent Infectionmentioning

confidence: 99%

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Cyprinid Herpesvirus 3

Boutier

Ronsmans

Rakus

et al. 2015

Advances in Virus Research

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Section: Latent Infectionsupporting

confidence: 76%

Section: Latent Infectionmentioning

confidence: 99%

Cyprinid Herpesvirus 3

Boutier

Ronsmans

Rakus

et al. 2015

Advances in Virus Research

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“…After a 30-day quarantine and acclimation period, fish found to have no clinical signs of CyHV-3 were used in our study. Koi with CyHV-3 latent infection were screened for CyHV-3 by nested PCR as reported previously (34). All koi were kept and maintained at 12°C in 4-ft diameter tanks at Oregon State University Salmon Disease Research Lab (OSU-SDL) in accordance with the Animal Care and Use Committee regulations.…”

Section: Methodsmentioning

confidence: 99%

“…NC_009127.1) available at NCBI. The primers used for screening for the presence of CyHV-3 DNA in koi WBC were selected as previously described (34). Real-time PCR primers specific for CyHV-3 were selected as previously described (2).…”

Section: Methodsmentioning

confidence: 99%

Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency

Reed

Izume

Dolan

et al. 2014

J Virol

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Cyprinid herpesvirus 3 (CyHV-3), commonly known as koi herpesvirus (KHV), is a member of the Alloherpesviridae, and is a recently discovered emerging herpesvirus that is highly pathogenic for koi and common carp. Our previous study demonstrated that CyHV-3 becomes latent in peripheral white blood cells (WBC). In this study, CyHV-3 latency was further investigated in IgM IMPORTANCEThis is the first demonstration that a member of the Alloherpesviridae, cyprinid herpesvirus 3 (CyHV-3), establishes a latent infection in the B cells of its host, Cyprinus carpio. In addition, this is the first report of identification of gene transcription during latency for a member of Herpesvirales outside Herpesviridae. This is also the first report that the hypothetical protein of latent transcript of CyHV-3 contains a consensus sequence with homology to a conserved domain of EBNA-3B from Epstein-Barr virus and ICP4 from herpes simplex virus 1, which are genes important for latency. These strongly suggest that latency is evolutionally conserved across vertebrates.

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“…KHV telah didiagnosis pada ikan koi dan ikan mas dan penularan KHV mengakibatkan tingkat kematian yang tinggi (McCleary et al, 2012;Xu et al, 2013;Cho et al, 2014;Weber et al, 2014).…”

Section: Pembahasanunclassified

Potential transmission test of GP25 vaccine in normal flora bacteria of common carp culture media

Nuryati¹,

Ali²,

Juliadiningtyas³

2015

Jurnal Akuakultur Indonesia

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PENDAHULUANWabah koi herpes virus atau KHV mulai diketahui terjadi awal tahun 1995−1996. Namun menurut Hedrick et al. (2005) KHV mulai lebih dikenal ketika diakui secara formal oleh Jerman pada tahun 1997 dan belum diidentifikasi sampai dengan tahun 1998. KHV dilaporkan menyebabkan kematian yang tinggi pada jenis ikan koi dan ikan mas di Israel dan USA (Pokorova et al., 2005). Wabah KHV diduga masuk ke Indonesia melalui ekspor ikan koi yang berasal dari Hongkong dan terjadi sejak bulan Maret pada tahun 2002 di daerah Blitar Jawa Timur (Sunarto et al., 2005).Penyakit KHV menyebabkan kematian massal dengan mortalitas 80−95% untuk kedua jenis ikan tersebut dan menyebabkan kerugian yang mencapai miliaran rupiah (Sunarto et al., 2005). KHV diketahui menyebabkan kerusakan pada kulit dan insang pada koi dan ikan mas. Ikan yang terkena KHV akan berenang secara acak dengan frekuensi pernapasan yang tinggi, insang bengkak, dan terjadi kerusakan pada kulit. ABSTRACTKoi herpesvirus (KHV) is a virus that infects common carp and koi Cyprinus carpio. KHV attacks most stadia of common carp and koi and causes mortality up to 80-95% of the population. One way to prevent the spread of KHV is by applying DNA vaccine. This research was conducted to test potential transmission of DNA vaccine encoding glycoprotein 25 (vaccine GP25) toward normal flora bacteria of media cultivation of common carp. Bacteria was isolated from pond water of common carp and tested for their sensitivity to ampicillin. Research was performed by adding vaccine GP25 to ampicillin-sensitive bacteria at a dose of 12.5 µg/100 µL and incubated at 28 °C for 30, 60, 180, and 300 minutes then plated on media containing ampicillin. The grown bacteria cells were tested for the existence of plasmid bearing gen GP25 through bacteria colony cracking. The results of this research showed that there was no bacteria contained plasmid bearing gen GP25.Keywords: DNA vaccines, common carp, vaccine safety ABSTRAKKoi herpesvirus (KHV) merupakan virus yang menginfeksi ikan mas dan koi Cyprinus carpio. KHV menyerang hampir semua stadia ikan mas dan koi dan menyebabkan kematian hingga 80-95% dari populasi. Salah satu cara penanggulangan penyebaran KHV adalah dengan penggunaan vaksin DNA. Penelitian ini dilakukan untuk menguji potensi transmisi vaksin DNA glikoprotein 25 (vaksin GP25) pada bakteri flora normal pada media budidaya ikan mas. Bakteri diisolasi dari kolam budidaya ikan mas dan diuji sensitivitasnya terhadap antibiotik ampisilin. Penelitian dilakukan dengan menambahkan vaksin GP25 ke larutan bakteri sensitif ampisilin dengan dosis 12,5 µg/100 µL dan diinkubasi pada suhu 28 °C selama 30, 60, 180, dan 300 menit kemudian disebar pada media mengandung antibiotik ampisilin. Sel bakteri yang tumbuh diuji untuk mendeteksi keberadaan plasmid pembawa gen GP25 dengan menggunakan metode seleksi koloni bakteri (colony cracking). Hasil penelitian ini menunjukkan bahwa tidak ada bakteri yang mengandung plasmid pembawa gen GP25.Kata kunci: keamanan vaksin, ikan mas, vaksin DNA Artikel O...

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Analysis of koi herpesvirus latency in wild common carp and ornamental koi in Oregon, USA

Cited by 39 publications

References 17 publications

Cyprinid Herpesvirus 3

Cyprinid Herpesvirus 3

Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency

Potential transmission test of GP25 vaccine in normal flora bacteria of common carp culture media

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