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Mk, Baxi; Ps, Reddy; An, Zakhartchouk; Idamakanti, Neeraja; Pyne, Caron; La, Babiuk; Sk, Tikoo

doi:10.1023/a:1008047225872

Cited by 9 publications

(3 citation statements)

References 28 publications

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“…In HAVs, the IVa2 protein has previously been shown to contribute to MLP activation (62). The IVa2 protein of BAV-3 is 376 amino acids long (6), which is shorter than the IVa2 proteins of other adenoviruses (45,61,69). The BAV-3 IVa2 protein shows amino acid identities of 29 to 69% to the IVa2 proteins of other adenoviruses (6) ( Table 3).…”

Section: Resultsmentioning

confidence: 93%

“…The pIX protein of HAVs is a structural component of the virion (67) and acts as a transcriptional activator (38a). The pIX protein of BAV-3 is 125 amino acids long (6,75), which is shorter than the pIX protein of HAV-2 (61) but longer than the pIX protein of CAV-1 (45). The BAV-3 pIX protein shows homologies of 16 to 28% to the pIX proteins of other adenoviruses (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…The E2B region of HAV-2 codes for DNA polymerase (Pol) and precursor terminal protein (pTP). As in HAV-2, the E2B region of BAV-3 codes for DNA Pol and pTP (6), which show significant homologies to the homologous proteins of other adenoviruses (Table 3). Sequence analysis of cDNA clones representing the DNA Pol and pTP of BAV-3 showed that the transcripts coding for them are 3Ј coterminal ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

Reddy

Idamakanti

Zakhartchouk

et al. 1998

J Virol

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The complete DNA sequence of bovine adenovirus type 3 is reported here. The size of the genome is 34,446 bp in length with a G+C content of 54%. All the genes of the early and late regions are present in the expected locations of the genome. However, the late-region genes are organized into seven families, instead of five as they are in human adenovirus type 2. The deduced amino acid sequences of open reading frames (ORFs) in the late regions and early region 2 (E2) and for IVa2 show higher degrees of homology, whereas the predicted amino acid sequences of ORFs in the E1, E3, and E4 regions and the pIX, fiber, and 33,000-molecular-weight nonstructural proteins show little or no homology with the corresponding proteins of other adenoviruses. In addition, the penton base protein lacks the integrin binding motif, RGD, but has an LDV motif instead of an MDV motif. Interestingly, as in other animal adenoviruses, the virus-associated RNA genes appear to be absent from their usual location. Sequence analysis of cDNA clones representing the early- and late-region genes identified splice acceptor and splice donor sites, polyadenylation signals and polyadenylation sites, and tripartite leader sequences.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

Reddy

Idamakanti

Zakhartchouk

et al. 1998

J Virol

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Construction and Characterization of E3-Deleted Bovine Adenovirus Type 3 Expressing Full-Length and Truncated Form of Bovine Herpesvirus Type 1 Glycoprotein gD

et al. 1998

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Using the homologous recombination machinery of E. coli, a 1.245-kb deletion was introduced in the E3 region of bovine adenovirus 3 (BAV3) genomic DNA cloned in a plasmid. Transfection of the restriction enzyme-excised, linear E3-deleted BAV3 genomic DNA into primary fetal bovine retina cells produced infectious virus (BAV3. E3d), suggesting that all the E3-specific open reading frames are nonessential for virus replication in vitro. Using a similar approach, we constructed replication-competent (BAV3.E3gD and BAV3. E3gDt) BAV3 recombinant expressing full-length (gD) or truncated (gDt) glycoprotein of bovine herpes virus 1. Recombinant gD and gDt proteins expressed by BAV3.E3gD and BAV3.E3gDt, respectively, were recognized by gD-specific monoclonal antibodies directed against conformational epitopes, suggesting that antigenicity of recombinant gD and gDt was similar to that of the native gD expressed in bovine herpes virus 1-infected cells. Intranasal immunization of cotton rats induced strong gD- and BAV3-specific IgA and IgG immune responses. These results suggest that replication-competent bovine adenovirus 3-based vectors have potential for the delivery of vaccine antigens to the mucosal surfaces of animals.

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Nucleotide Sequence and Transcription Map of Porcine Adenovirus Type 3

et al. 1998

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The complete nucleotide sequence of porcine adenovirus type 3 was determined and a transcriptional map for the genome was constructed. The size of the genome is 34094 bp in length with an unusually high G + C content (63.7%), the highest thus far reported for any adenovirus. Overall organization of the genome is similar to that for previously sequenced adenoviral DNAs, but there also were distinct differences. The late regions genes are organized into six families, instead of five as they are in human adenovirus type 2. In contrast to bovine adenovirus type 3 and ovine adenovirus, which lack virion-associated RNA genes, the nucleotide sequence analysis of the viral genome indicates that it encodes one short VA RNA species. With the exception of the fiber and a 33-kDa nonstructural protein, the predicted amino acid sequences of the open reading frames in the late regions and the E2 region and IVa2 exhibited a high level of homology, whereas the deduced amino acid sequences of ORFs in E1, E3, and E4 regions, and the pIX showed a lesser homology with the corresponding proteins of other adenoviruses. The proteins V, VII, and IX are unusually long, and the protein VII lacks the consensus protease cleavage site. Genomic and cDNA sequence analysis has identified promoters, cap sites, intron-exon boundaries, polyadenylation signals, and polyadenylation sites in the viral genome.

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Untitled

Cited by 9 publications

References 28 publications

Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

Construction and Characterization of E3-Deleted Bovine Adenovirus Type 3 Expressing Full-Length and Truncated Form of Bovine Herpesvirus Type 1 Glycoprotein gD

Nucleotide Sequence and Transcription Map of Porcine Adenovirus Type 3

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