Amperometric flow system for blood glucose determination using an immobilized enzyme magnetic reactor

Hernández, Prisciliano; Rodríguez, José A.; Galán, Carlos A.; Castrillejo, Y.; Barrado, E.

doi:10.1016/j.bios.2012.08.033

Cited by 14 publications

(9 citation statements)

References 20 publications

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“…[14][15][16][17][18][19][20][21][22][23][24] For example, Hernandez and coworkers 16 developed an amperometric flow system based on a magnetically immobilized glucose oxidase, for the determination of glucose in blood samples. The amino modified, silica coated, magnetite was used to bind the enzyme with the glutaraldehyde protocol.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Riccò¹,

Doherty²,

Falcaro³

2014

j nanosci nanotechnol

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Beta-Glucosidase has been chosen as a model biomolecule to establish a general protocol for binding enzymes on both ferromagnetic and superparamagnetic nano-particles for sensing applications. Using EDC (1-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide) or SMCC (Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) as coupling agents, we compared two different methods for the fabrication of enzyme-decorated magnetic nanoparticles. We identified the best conditions for the preparation of a responsive bioactive magnetic system comparing different covalent bio-grafting protocols. The enzymatic test has been performed using beta-Glucosidase. The systems were characterized using scanning electron microscopy, infrared spectroscopy, and the enzyme loading was measured by a glucose assay in the presence of the enzyme-decorated magnetic particles. Although the faster response of ferromagnetic particles to the magnetic field, the assay results suggested that the superparamagnetic particles are more efficient carriers. In fact, the best enzymatic activity was measured on superparamagnetic systems that have the further advantage of preventing aggregation induced by the residual magnetization. Hence, beta-Glucosidase coated magnetic nanospheres could provide an attractive system suitable for the cleavage and the rapid evaluation of glycoside levels in natural products, measuring the liberated glucose without the need for specialised instrumentation. Moreover, the magnetic particles allow the subsequent collection of enzymes for further analysis, such as its use in portable fast screening kits or devices.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Riccò¹,

Doherty²,

Falcaro³

2014

j nanosci nanotechnol

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“…Wires incorporated into microfluidic channels can serve the same purpose (Blanes et al, 2007). The other typical method to retain microparticles is the usage of magnetic particles (Hernandez et al, 2013;Lin et al, 2013;Sheng et al, 2012). These can be kept at a desired position using an external magnetic field, making additional features in the channels unnecessary.…”

Section: Fabricationmentioning

confidence: 97%

“…Typical detection techniques employed in systems with microparticles are either amperometry (Godino et al, 2010;Hernandez et al, 2013;Ito et al, 2007Ito et al, , 2011Sheng et al, 2012) or optical techniques such as fluorescence measurements (Jang and Koh, 2010;Kim et al, 2009). The detection limits and linear ranges for these methods are in the same range as those reported in Section 3, making the immobilization strategies comparable in terms of measurement performance.…”

Section: Characteristics and Performancementioning

confidence: 98%

See 1 more Smart Citation

Microfluidic enzymatic biosensing systems: A review

Mross

Pierrat

Zimmermann

et al. 2015

Biosensors and Bioelectronics

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“…Optimization of the system with TPD involves 5 steps, I) identifying the output variable to optimize, II) identifying and selecting factors that affect the system, III) selecting the appropriate orthogonal array and assigning adequate settings to the chosen factors, IV) analyzing the data and determining the optimum settings, and V) conducting a confirmatory experiment under the optimal conditions obtained [30].…”

Section: Optimization Of Background Electrolyte Compositionmentioning

confidence: 99%

Importance of Treatment Process on the Analysis of Penicillins in Milk Samples by Capillary Electrophoresis

Rodríguez¹,

Martínez²,

Ibarra³

et al. 2015

Field Effect Electroosmosis - A Novel Phenomenon in Electrokinetics and Its Applications in Capillary Electrophoresis

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Amperometric flow system for blood glucose determination using an immobilized enzyme magnetic reactor

Cited by 14 publications

References 20 publications

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Microfluidic enzymatic biosensing systems: A review

Importance of Treatment Process on the Analysis of Penicillins in Milk Samples by Capillary Electrophoresis

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