First detection and molecular characterization of Ehrlichia canis from dogs in Nigeria

Kamani, Joshua; Lee, Chung-Chan; Haruna, A. M.; Chung, Ping-Jun; Weka, Paul R.; Chung, Yang-Tsung

doi:10.1016/j.rvsc.2012.07.031

Cited by 27 publications

(20 citation statements)

References 27 publications

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“…Doyle et al (2005) characterized the TRP36 gene of American (US), Brazilian (Br) and Cameroonian E. canis strains and reported a Tandem Repeat (TR) sequence of nine amino acids (TEDSVSAPA) (US genotype; Table 1). This TR amino acid sequence was completely preserved in numerous E. canis strains around the world (Zhang et al, 2008;Hsieh et al, 2010;Kamani et al, 2013), including the new isolates from South Africa reported by Zweygarth et al (2014). Only one divergent E. canis TR sequence has been identified in an Israeli strain (Ranana strain) "TEDPVSATA".…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 92%

“…The 16S rRNA gene sequence is 99.4-100% identical among isolates from numerous countries, thus providing little information about the overall diversity of this organism (Yu et al, 2007;Aguiar et al, 2008;Zhang et al, 2008;Hsieh et al, 2010;Kamani et al, 2013). On the other hand, the molecular characterization of different sequences from the Tandem Repeat Protein (TRP36) gene of E. canis isolates obtained of naturally infected dogs has revealed variable divergence.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

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Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil

Aguiar

Melo

2015

Ticks and Tick-borne Diseases

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Section: Contents Lists Available At Sciencedirectmentioning

confidence: 92%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil

Aguiar

Melo

2015

Ticks and Tick-borne Diseases

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“…canis is the primary causal agent of Canine Monocytic Ehrlichiosis (CME), a worldwide tick-borne disease (Kamani et al, 2013;Stich et al, 2008); it is an obligate intracellular gram-negative bacterium that multiply in eukaryotic cells, like monocytes and macrophages, developing leucopenia and thrombocytopenia (Stich et al 2008). CME can be divided in an acute phase, beginning from 8 to 20 days after infection, involving anemia, anorexia, ataxia, conjunctivitis, depression, fever, leucopenia, ocular discharge, thrombocytopenia and vomiting that end with a partial recovery of the dog, followed by an months-to-years subclinical phase.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Ehrlichia canis in dogs from Monterrey, Mexico

Salinas-Melendez¹,

Cantú-Martínez²,

Wong-González³

et al. 2015

Afr. J. Microbiol. Res.

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Infection by Ehrlichia canis in dogs causes the worldwide tick-borne disease called canine monocytic ehrlichiosis (CME), and the presence of E. canis has been serologically demonstrated in all continents, with prevalence ranging from 0.2 to 80%. In southern Mexico, a prevalence of 44% was found, whereas in the northwest part of the country it varies from 21 to 49%. In the present study, a commercial kit for the detection of antibodies against E. canis was used in 391 dogs from the city of Monterrey, which is located at northeast of Mexico. A total of 54 samples were positive, giving a prevalence of 13%. According to sex, prevalence was 14% for males and 13% for females. Positive animals varied in age from 21 to 132 months old and only 10 of them presented ticks. As in the southern and northwest parts of Mexico, CME is present in northeast region, although with a lower prevalence.

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“…Consequently, much less is known about the other genes of this microorganism. Molecular characterization of 16S rRNA has provided information about strain diversity and suggests a high level of conservation [16,24]. Little is known about the disulfide bond formation protein gene ( dsb ) or the role of the inter- and intramolecular disulfide bonds in the overall Ehrlichia cell envelope structure.…”

Section: Introductionmentioning

confidence: 99%

Isolation,in vitropropagation, genetic analysis, and immunogenic characterization of anEhrlichia canisstrain from southeastern Brazil

et al. 2014

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Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlândia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28 partial sequence for the E. canis Uberlândia strain differed at several nucleotides from other sequences available in GenBank. To confirm the antigenic profile of the Uberlândia strain, an indirect immunofluorescence assay against E. canis antigens was performed using dog sera collected from two different areas in Brazil (Uberlândia and São Paulo). The results suggest that both antigens were able to identify animals seropositive for E. canis in Brazil since these Brazilian strains appear to be highly conserved.

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First detection and molecular characterization of Ehrlichia canis from dogs in Nigeria

Cited by 27 publications

References 27 publications

Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil

Divergence of the TRP36 protein (gp36) in Ehrlichia canis strains found in Brazil

Seroprevalence of Ehrlichia canis in dogs from Monterrey, Mexico

Isolation,in vitropropagation, genetic analysis, and immunogenic characterization of anEhrlichia canisstrain from southeastern Brazil

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