220	Identification of a novel HMW glutenin subunit and compari-son of its amino acid sequence with those of homologous sub-units

Yan, Zehong; Wan, Yongfang; Liu, Kunfan; Zheng, Youliang; Wang, Daowen

doi:10.1360/02tb9053

Cited by 93 publications

(58 citation statements)

References 19 publications

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“…Genomic DNA, total RNA, and cDNA preparation DNA was isolated from 1 g of 10-day-old seedling coleoptiles according to the methods of Yan et al (2002). Total RNA was extracted from ~0.5 g of coleoptiles with the Tiangen RNA prep Pure Plant Kit (Tiangen Company, Beijing, China).…”

Section: Plant Materialsmentioning

confidence: 99%

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Wei

Chen

et al. 2017

Cereal Research Communications

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Red coleoptile is an easily observed trait in Triticum aestivum and can provide some protection against stress. Here, TaMYB-A1 or TuMYB-A1, homologous to TaMYB-D1, which controls red coleoptile formation in the common wheat cultivar 'Gy115', was isolated from eight T. aestivum and 34 T. urartu cultivars. The genome sequence of TaMYB-A1 was 867 bp with an intron of 93 bp, which was similar to the MYBs regulating anthocyanin biosynthesis in T. aestivum but different from other MYB transcription factors regulating anthocyanin biosynthesis. TaMYB-A1 had an integrated DNA-binding domain of 102 amino acids and a transcriptional domain of 42 amino acids, which was responsible for regulating anthocyanin biosynthesis. TaMYB-A1 was assigned to the same branch as the MYBs regulating anthocyanin biosynthesis in a phylogenetic tree. A transient expression analysis showed that TaMYB-A1 induced 'Opata' coleoptile cells to synthesize anthocyanin with the help of ZmR. A non-functional allele of TaMYB-a1 existed in common wheat cultivars containing rc-a1. One single nucleotide was deleted 715 bp after the start codon in TaMYB-a1 compared with TaMYB-A1. The deletion caused a frame shift mutation, destroyed the DNA transcription activator domain, and resulted in TaMYB-a1 losing its ability to regulate anthocyanin biosynthesis in 'Opata' coleoptile cells. Those cultivars with functional TaMYB-A1 or TuMYB-A1 have red coleoptiles. The isolation of TaMYB-A1 should aid in understanding the molecular mechanisms of coleoptile traits in T. aestivum.

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Section: Plant Materialsmentioning

confidence: 99%

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Wei

Chen

et al. 2017

Cereal Research Communications

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“…Genomic DNA, total RNA, and cDNA preparation DNA was isolated from 1 g of 10-day-old seedlings according to the methods of Yan et al (2002). Total RNA was extracted from approximately 0.5 g of coleoptiles with the Tiangen RNAprep Pure Plant Kit (Tiangen, China).…”

Section: Plant Materialsmentioning

confidence: 99%

Identification of a candidate gene for Rc-D1, a locus controlling red coleoptile colour in wheat

Wang¹,

Hou²,

Zhang³

et al. 2016

Cereal Research Communications

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“…The common wheat cultivars Chuanyu 12 (subunit 1Ax1, 7 + 8, 5 + 10), Longfumai 1 (1Ax2*, 7 + 8, 5 + 10), and Chinese Spring (null, 7+8, 2+12) were used as standards. Seed protein extraction and SDS-PAGE were conducted as described by Yan et al (2002). DNA extraction, PCR amplification and DNA sequencing analysis DNA isolation was carried out from young leaf tissue using the Plant Genomic DNA Kit (Tiangen, Beijing, China).…”

Section: Plant Materials and Sds-page Analysismentioning

confidence: 99%

Molecular characterization of differentTriticum monococcumssp.monococcum Glu-A1^mxalleles

Li¹,

Li²,

Zeng³

et al. 2016

Cereal Research Communications

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High-molecular-weight glutenin subunits (HMW-GSs) are important seed storage proteins associated with bread-making quality in common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD). Variation in the Glu-A1x locus in common wheat is scare. Diploid Triticum monococcum ssp. monococcum (2n = 2x = 14, A m A m ) is the first cultivated wheat. In the present study, allelic variations at the Glu-A1 m x locus were systematically investigated in 197 T. monococcum ssp. monococcum accessions. Out of the 8 detected Glu-A1 m x alleles, 5 were novel, including Glu-A1 m -b, Glu-A1 m -c, Glu-A1 m -d, Glu-A1 m -g, and Glu-A1 m -h. This diversity is higher than that of common wheat. Compared with 1Ax1 and 1Ax2*, which are present in common wheat, these alleles contained three deletions/insertions as well as some single nucleotide polymorphism variations that might affect the elastic properties of wheat flour. New variations in T. monococcum probably occurred after the divergence between A and A m and are excluded in common wheat populations. These allelic variations could be used as novel resources to further improve wheat quality.

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220 Identification of a novel HMW glutenin subunit and compari-son of its amino acid sequence with those of homologous sub-units

Cited by 93 publications

References 19 publications

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Identification of a candidate gene for Rc-D1, a locus controlling red coleoptile colour in wheat

Molecular characterization of differentTriticum monococcumssp.monococcum Glu-A1^mxalleles

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220 Identification of a novel HMW glutenin subunit and compari-son of its amino acid sequence with those of homologous sub-units

Cited by 93 publications

References 19 publications

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Identification of a candidate gene for Rc-D1, a locus controlling red coleoptile colour in wheat

Molecular characterization of differentTriticum monococcumssp.monococcum Glu-A1mxalleles

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Molecular characterization of differentTriticum monococcumssp.monococcum Glu-A1^mxalleles