22 week assessment of bladder acellular matrix as a bladder augmentation material in a porcine model

Brown, Angela Demke; Farhat, Walid A.; Merguerian, Paul A.; Wilson, Gregory J.; Khoury, Antoine E.; Woodhouse, Kimberly A.

doi:10.1016/s0142-9612(01)00350-7

Cited by 142 publications

(111 citation statements)

References 37 publications

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“…1 Small intestinal submucosa (SIS) has been utilized previously to engineer the urinary bladder wall with and without cell seeding. Previous studies have shown that to maintain graft size, cell seeding of the SIS prior to implantation is necessary.…”

Section: Introductionmentioning

confidence: 99%

Generating Elastin-Rich Small Intestinal Submucosa–Based Smooth Muscle Constructs Utilizing Exogenous Growth Factors and Cyclic Mechanical Stimulation

Heise

Ivanova

Parekh

et al. 2009

Tissue Engineering Part A

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Successful approaches to tissue engineering smooth muscle tissues utilize biodegradable scaffolds seeded with autologous cells. One common problem in using biological scaffolds specifically is the difficulty of inducing cellular penetration and controlling de novo extracellular matrix deposition=remodeling in vitro. Our hypothesis was that small intestinal submucosa (SIS) exposed to specific mechanical stimulation regimes would modulate the synthesis of de novo collagen and elastin by bladder smooth muscle cells (BSMC) within the SIS matrix. We further hypothesized that the cytokines vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2), two key growth factors involved in epithelial mesenchymal signaling, will promote the cellular penetration into SIS necessary for mechanical stimulation. BSMC were seeded at 0.5Â10 6 cells=cm 2 onto the luminal side of SIS specimens. VEGF (10 ng=mL) and FGF-2 (5 ng=mL) were added to each insert in the media every other day for up to 7 days in static culture. Following static culture, specimens were stretched stripbiaxially under 15% peak strain at either 0.5 or 0.1 Hz for an additional 7 days. Following the culture period, specimens were assayed histologically and biochemically for cellular penetration, proliferation, elastin, collagen, and protease activity. Histological analyses demonstrated that in standard culture media, BSMC remained on the surface of the SIS while both FGF-2 and VEGF profoundly promoted ingrowth of the BSMC into the SIS. The penetration of the cells in response to these cytokines was confirmed using a Transwell assay. Following cellular penetration, BSMC produced significant amounts of elastic fibers under cyclic mechanical stretching at 0.1 Hz under 15% stretch, as evidenced by colorimetric assay and histology using a Verhoeff-Van Gieson stain. Protease activity was assessed in the media and found to be statistically increased in static culture following FGF-2 treatment. These findings demonstrate, for the first time, the capability of BSMC to produce histologically apparent elastin fibers in vitro. Moreover, our results suggest that a strategy involving growth factors and controlled mechanical stimulation may be used to engineer functional, elastin-rich tissue replacements using decellularized biologically derived scaffolds.

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Section: Introductionmentioning

confidence: 99%

Generating Elastin-Rich Small Intestinal Submucosa–Based Smooth Muscle Constructs Utilizing Exogenous Growth Factors and Cyclic Mechanical Stimulation

Heise

Ivanova

Parekh

et al. 2009

Tissue Engineering Part A

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“…Multilayered bladder growth was seen at 1 month and included urothelium, angiogenesis and organized smooth muscle. At longer time intervals, graft shrinkage and a lack of central organized smooth muscle were observed, 23 likely consistent with inadequate neovascularization. Thus, while unseeded SIS and bladder ECM have shown the ability to participate in the regenerative process, several limitations have prevented this technology from progressing to clinical implementation.…”

Section: Unseeded Technologymentioning

confidence: 88%

Tissue engineering in urology

Matoka

Cheng

2013

CUAJ

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Tissue engineering encompasses a multidisciplinary approach geared toward the development of biological substitutes designed to restore and maintain normal function in diseased or injured tissues. This article reviews the basic technology that is used to generate implantable tissue-engineered grafts in vitro that will exhibit characteristics in vivo consistent with the physiology and function of the equivalent healthy tissue. We also examine the current trends in tissue engineering designed to tailor scaffold construction, promote angiogenesis and identify an optimal seeded cell source. Finally, we describe several currently applied therapeutic modalities that use a tissue-engineered construct. While notable progress has clearly been demonstrated in this emerging field, these efforts have not yet translated into widespread clinical applicability. With continued development and innovation, there is optimism that the tremendous potential of this field will be realized.Can Urol Assoc J 2009;3(5):403-8 Résumé L'ingénierie tissulaire englobe une approche multidisciplinaire axée sur le développement de substituts biologiques en vue de rétablir et de maintenir la fonction normale de tissus lésés. L'article qui suit passe en revue la technologie fondamentale utilisée pour générer des greffons implantables produits par ingénierie in vitro et possédant des caractéristiques in vivo correspondant aux tissus sains équivalents sur les plans physiologique et fonctionnel. Nous examinons également les tendances actuelles en ingénierie tissulaire visant à adapter des échafaudages tissulaires, à promouvoir l'angiogenèse et à dégager une source optimale de cellules implantables. Enfin, nous décrivons plusieurs modalités thérapeu-tiques actuellement mises en application utilisant un échafaudage créé par ingénierie tissulaire. En dépit de progrès remarquables dans ce domaine en effervescence, les efforts déployés ne se sont pas encore traduits par une applicabilité clinique étendue. Des développements et des percées continus permettent d'être optimiste face au potentiel prodigieux de ce domaine.

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“…Autologous materials have proven difficult to reproduce as a result of increased patient morbidity associated with the graft harvest (56). The increased cost in allograft and xenograft material production, in addition to the risk of disease transmission and varied mechanical strength, is crucial in their limited clinical application (56,57). Furthermore, the use of natural decellularised matrices requires tissue that exhibits no principal pathological change, and is therefore unfeasible in certain patients (58,59).…”

Section: Naturally Derived Matricesmentioning

confidence: 99%

Bladder reconstruction: The past, present and future

2015

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Abstract. Ileal conduit urinary diversion is the gold standard treatment for urinary tract reconstruction following cystectomy. This procedure uses gastrointestinal segments for bladder augmentation, a technique that is often associated with significant complications. The substantial progression in the fields of tissue engineering and regenerative medicine over the previous two decades has resulted in the development of techniques that may lead to the construction of functional de novo urinary bladder substitutes. The present review identifies and discusses the complications associated with current treatment options post-cystectomy. The current techniques, achievements and perspectives of the use of biomaterials and stem cells in the field of urinary bladder reconstruction are also reviewed.

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22 week assessment of bladder acellular matrix as a bladder augmentation material in a porcine model

Cited by 142 publications

References 37 publications

Generating Elastin-Rich Small Intestinal Submucosa–Based Smooth Muscle Constructs Utilizing Exogenous Growth Factors and Cyclic Mechanical Stimulation

Generating Elastin-Rich Small Intestinal Submucosa–Based Smooth Muscle Constructs Utilizing Exogenous Growth Factors and Cyclic Mechanical Stimulation

Tissue engineering in urology

Bladder reconstruction: The past, present and future

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