21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometer: A National Resource for Ultrahigh Resolution Mass Analysis

Abstract

Abstract. We describe the design and initial performance of the first 21 tesla Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The 21 tesla magnet is the highest field superconducting magnet ever used for FT-ICR and features high spatial homogeneity, high temporal stability, and negligible liquid helium consumption. The instrument includes a commercial dual linear quadrupole trap front end that features high sensitivity, precise control of trapped ion number, and collisional and electron transfer dissociation. A third linear quadrupole trap offers high ion capacity and ejection efficiency, and rf quadrupole ion injection optics deliver ions to a novel dynamically harmonized ICR cell. Mass resolving power of 150,000 (m/Δm 50% ) is achieved for bovine serum albumin (66 kDa) for a 0.38 s detection period, and greater than 2,000,000 resolving power is achieved for a 12 s detection period. Externally calibrated broadband mass measurement accuracy is typically less than 150 ppb rms, with resolving power greater than 300,000 at m/z 400 for a 0.76 s detection period. Combined analysis of electron transfer and collisional dissociation spectra results in 68% sequence coverage for carbonic anhydrase. The instrument is part of the NSF High-Field FT-ICR User Facility and is available free of charge to qualified users.

Comprehensive analysis of tropomyosin isoforms in skeletal muscles by top-down proteomics

“…We envision the sensitivity of this LC-MS+ method could be improved with the use of more sensitive mass spectrometers as well as the new developments on the ion isolation and dissociation techniques. Note that the LTQ/FT mass spectrometer used in this study is 8-year-old and there are now new generation of high-resolution high sensitivity Fourier transform ion cyclotron resonance and Orbitrap mass spectrometers available (Eliuk and Makarov 2015; Hendrickson et al 2015; Kostyukevich et al 2012; Xian et al 2012). In addition, only a single charge state can be isolated for sequencing using the current LTQ/FT mass spectrometer.…”

Extreme isomeric complexity of dissolved organic matter found across aquatic environments

“…It is an ultra-complex mixture of compounds that provides the ultimate test of the capabilities of analytical chemistry (Rodgers et al 2005;Dittmar and Stubbins 2014), and investigations into its nature have been confounded by its extreme molecular complexity. High-resolution mass spectrometry (HRMS) is able to resolve many thousands of molecular masses from complex natural mixtures of organic compounds (Marshall et al 1998;Riedel and Dittmar 2014;Hendrickson et al 2015), but is unable to differentiate between structural isomers of a molecular formula.Most recent research has utilized advanced visualization or multivariate statistical approaches to interpret HRMS data (Wu et al 2004;Sleighter et al 2010;Kellerman et al 2015), but when the HRMS data are not combined with more This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. …”

“…It is an ultra-complex mixture of compounds that provides the ultimate test of the capabilities of analytical chemistry (Rodgers et al 2005;Dittmar and Stubbins 2014), and investigations into its nature have been confounded by its extreme molecular complexity. High-resolution mass spectrometry (HRMS) is able to resolve many thousands of molecular masses from complex natural mixtures of organic compounds (Marshall et al 1998;Riedel and Dittmar 2014;Hendrickson et al 2015), but is unable to differentiate between structural isomers of a molecular formula.…”

Bacterial Electron Transfer Chains Primed by Proteomics

“…An ICR cell is located at the centre (bore) of a high magnetic field superconducting magnet, which is in the range of 3-21 Tesla (T) (Hendrickson et al, 2015). First, ions are injected into the ICR cell and trapped by applying a trapping voltage to the front and back plates.…”