20. Early Extracellular Matrix Synthesis by Bone Cells

Davies, John E.; Ottensmeyer, Patrick Frank; Shen, Xiangying; Hashimoto, Michiaki; Peel, Sean

doi:10.3138/9781442671508-024

Cited by 19 publications

(22 citation statements)

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“…While biochemical aspects of osteogenic cell or tissue cultures have been well documented, comparatively less is known about the structural aspects of these cultures, and in particular, about the precise distribution and interrelationships of the collagenous and non-collagenous components of the mineralized matrix. It has been shown previously that the first extracellular matrix produced by bone marrow-derived osteogenic cells in vitro is an afibrillar, cement line-like material deposited at the cell-culture dish interface (Davies et al, 1991b;Shen et al, 19931, a result consistent with what is observed at natural and surgically-created interfaces in vivo (reviewed in McKee and Nanci, this issue). Interfacial matrix was also observed in the present study with calvaria-derived …”

Section: Structural and Compositional Characterizationsupporting

confidence: 72%

“…Bone sialoprotein, and particularly osteopontin, are synthesized very early during this process and constitute the major non-collagenous bone proteins incorporated into the mineralized matrix produced in rat calvaria-and marrow-derived osteogenic cell cultures (Kasugai et al, 1991Nagata et al, 1991). Furthermore, it has been proposed that the initial non-collagenous matrix assembles as a cement line-like structure at the interface with the culture dish substrate (Davies et al, 1991b;Shen et al, 1993). This sequence of events clearly shows parallels with osteogenic processes occurring in vivo (reviewed in McKee and Nanci, 1993, this issue).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrastructural characterization and immunolocalization of osteopontin in rat calvarial osteoblast primary cultures

Nanci

Zalzal

Gotoh

et al. 1996

Microsc. Res. Tech.

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Section: Structural and Compositional Characterizationsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Ultrastructural characterization and immunolocalization of osteopontin in rat calvarial osteoblast primary cultures

Nanci

Zalzal

Gotoh

et al. 1996

Microsc. Res. Tech.

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“…Detailed analysis of the crystals by energy dispersive x-rays spectroscopy showed that these structures were containing Ca and P elements. Such a substratum produced by differentiating osteoblasts was described as an anchorage for calcifying collagen fibers synthesized by the same cells in a rat model [74]. Signs of mineralization are fundamental in perspective to use primary fetal bone cells for artificial bone synthesis.…”

Section: Discussionmentioning

confidence: 99%

Fetal bone cells for tissue engineering

Montjovent

Burri

Mark

et al. 2004

Bone

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We envision the use of human fetal bone cells for engineered regeneration of adult skeletal tissue. A description of their cellular function is then necessary. To our knowledge, there is no description of human primary fetal bone cells treated with differentiation factors. The characterization of fetal bone cells is particularly important as the pattern of secreted proteins from osteoblasts has been shown to change during aging. In the first part of this work, human primary fetal bone cells were compared to adult bone cells and mesenchymal stem cells for their ability to proliferate and to differentiate into osteoblasts in vitro. Cell proliferation, gene expression of bone markers, alkaline phosphatase (ALP) activity, and mineralization were analyzed during a time-course study. In the second part of this paper, bone fetal cells behavior exposed to osteogenic factors is further detailed. The doubling time of fetal bone cells was comparable to mesenchymal stem cells but significantly shorter than for adult bone cells. Gene expression of cbfa-1, ALP, a1 chain of type I collagen, and osteocalcin were upregulated in fetal bone cells after 12 days of treatment, with higher inductions than for adult and mesenchymal stem cells. The increase of ALP enzymatic activity was stronger for fetal than for adult bone cells reaching a maximum at day 10, but lower than for mesenchymal stem cells. Importantly, the mineralization process of bone fetal cells started earlier than adult bone and mesenchymal stem cells. Proliferation of fetal and adult bone cells was increased by dexamethasone, whereas 1a,25-dihydroxyvitamin D 3 did not show any proliferative effect. Mineralization studies clearly demonstrated the presence of calcium deposits in the extracellular matrix of fetal bone cells. Nodule formation and calcification were strongly increased by the differentiation treatment, especially by dexamethasone. This study shows for the first time that human primary fetal bone cells could be of great interest for bone research, due to their fast growth rate and their ability to differentiate into mature osteoblasts. They represent an interesting and promising potential for therapeutic use in bone tissue engineering.

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“…Selective microanalysis enables the detection of specific elements present on the surface. For example, phosphorus and calcium, which are present in calcium phosphate compounds produced during mineralization by osteoblasts, 29 can be analyzed.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

et al. 2005

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The aim of this investigation was to test the biocompatibility of three-dimensional bioresorbable foams made of poly(L-lactic acid) (PLA), alone or filled with hydroxyapatite (HA) or ␤-tricalcium phosphate (␤-TCP), with human primary osteoblasts, using a direct contact method. Porous constructs were processed by supercritical gas foaming, after a melt-extrusion of ceramic/polymer mixture. Three neat polymer foams, with pore sizes of 170, 310, and 600 m, and two composite foams, PLA/5 wt% HA and PLA/5 wt% ␤-TCP, were examined over a 4-week culture period. The targeted application is the bone tissue-engineering field. For this purpose, human fetal and adult bone cells were chosen because of their highly osteogenic potential. The association of fetal bone cells and composite scaffold should lead to in vitro bone formation. The polymer and composite foams supported adhesion and intense proliferation of seeded cells, as revealed by scanning electron microscopy. Cell differentiation toward osteoblasts was demonstrated by alkaline phosphatase (ALP) enzymatic activity, ␥-carboxylated Gla-osteocalcin production, and the onset of mineralization. The addition of HA or ␤-TCP resulted in higher ALP enzymatic activity for fetal bone cells and a stronger production of Gla-osteocalcin for adult bone cells.

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20. Early Extracellular Matrix Synthesis by Bone Cells

Cited by 19 publications

References 0 publications

Ultrastructural characterization and immunolocalization of osteopontin in rat calvarial osteoblast primary cultures

Ultrastructural characterization and immunolocalization of osteopontin in rat calvarial osteoblast primary cultures

Fetal bone cells for tissue engineering

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

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