2-Week and 13-Week Inhalation Studies of Aerosolized Glycerol in Rats

Interspecies Variations in the Histology of Toxicologically Important Areas in the Larynges of CRL:CD Rats and Syrian Golden Hamsters*†

“…Our studies (5,6,18,22) have supported the conclusion of Lewis (11,13) and Gopinath et al (8) that the epithelium lining the base of the epiglottis is the area of the laryngeal mucosa in laboratory rodents most susceptible to changes induced by inhaled irritants.…”

“…As in the nose, the predilection of these sites for lesions in rodents is probably due to airflow characteristics and regional epithelial sensitivity (4,8,11,13 (5,6,22) or glycerol (18). Sources, housing, and necropsy procedures for the animals of these studies have been reported previously (5,6,18,22 …”

Respiratory System of Rats Exposed to Pollutants arising out of Heating Residual Glycerol

“…Article similar to ours was conducted by Renne et al [16], where they conducted a study of glycerol aerosol inhalation for 2 and 13 weeks, with different concentrations of glycerol (0.1, 1.93 and 3.91 mg/L for 2 weeks and 0, 0.033, 0.167 and 0.662 mg/L for 13 weeks) in rats and were mainly a result the development of squamous metaplasia of the epiglottis of the epithelium in aerosol inhalation concentration of 0.167 mg glycerol/L in the group submitted to 13 weeks of inhalation. Studies have demonstrated that mice sensitized with ovalbumin and exposed to diesel exhaust gases 12 hours a day, 5 days per week for 5 weeks had allergic inflammation and airway hyper responsiveness, and a significant increase in respiratory system resistance [17] and the intratracheal administration of diesel exhaust particles (SDP) sensitization with ovalbumin (OVA) in mice induces inflammation and hyperresponsiveness of the airways and mucus hypersecretion [18].…”

Safety evaluation and risk assessment of electronic cigarettes as tobacco cigarette substitutes: a systematic review

“…Evidence for the potential harm of inhaled glycerol is sparse. A study using Sprague-Dawley rats found minimal to mild squamous metaplasia of the epiglottis epithelium in the high-dose group only, without any changes observed in lungs or other organs [Renne et al 1992]. No comparative set of experiments with cigarette smoke was included, but it is well known that exposure to tobacco smoke in similar animal models leads to dramatic changes in the lungs, liver and kidneys [Czekaj et al 2002].…”