2-Deoxycastasterone, a New Brassinolide-related Bioactive Steroid fromPinusPollen

Detection of Brassinolide and Castasterone in Alnus glutinosa (European Alder) Pollen by Mass Spectrometry/Mass Spectrometry

“…(2), the probable biogenetic precursor of 1, has been found in these same plants (2)(3)(4)(5)(6) plus seed of Phaseolus vulgaris (7) and pollen of Pinus tbunbergii (8).…”

“…Among several other biologically active brassinolide analogs also found in these plants, reports of 2-deoxycastasterone or typhasterol in cattail (Typha latifolia) pollen (9) and pine pollen (8) are noteworthy because they represent other pollen sources of these growth regulators. The amounts of 2-deoxycastasterone isolated (0.068 and 0.09 mg/ kg of pollen) are near the quantities of brassinolide (0.1 mg/kg) obtained from rape pollen (1).…”

Purification and separation of eight steroidal plant-growth regulators from Dolichos lablab seed.

“…The extract from immature seed of Dolichos lablab contains high levels of brassinolide activity as determined by the rice-lamina inclination test. Wehave preliminarily reported the isolation and structures of new brassinolide-related steroidal growth regulators, dolicholide (2), dolichosterone (6), homodolicholide (3) and homodolichosterone (7), from the seed, as well as the identification of castasterone (5) and brassinolide (1).1~3)…”

Purification and Separation of Eight Steroidal Plant-growth Regulators fromDolichos lablabSeed

“…We have preliminarily reported the isolation and structures of new brassinolide-related steroidal growth regulators, dolicholide (2), dolichosterone (6), homodolicholide (3) and homodolichosterone (7), from the seed, as well as the identification of castasterone (5) and brassinolide (1).1- 3) We describe here the details of the purification procedures of the steroidal growth regulators from the Dolichos seed. Recent findings of the occurrence of 6-deoxocastasterone (9) and 6-deoxodolichosterone (10) are also described.…”