Detection of Brassinolide and Castasterone in Alnus glutinosa (European Alder) Pollen by Mass Spectrometry/Mass Spectrometry

Plattner, Ronald D.; Taylor, Scott L.; Grove, Michael D.

doi:10.1021/np50045a033

Cited by 24 publications

(4 citation statements)

References 12 publications

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“…The d6 type, in which all of the internodes except the uppermost are reduced, could indicate that the uppermost internode is exposed to more BRs than is the fourth internode. The timing of the elongation of the uppermost internode corresponds to the development of anthers in the flowers, organs that in many plants have been observed to contain high amounts of BRs (Grove et al, 1979;Plattner et al, 1986;Suzuki et al, 1986;Ikekawa et al, 1988;Takatsuto et al, 1989;Gamoh et al, 1990). Presumably, large quantities of BRs could move down from the anthers to lower organs, such as the uppermost internode, and there induce internode elongation.…”

Section: Internodes Differ In Their Sensitivity To Brsmentioning

confidence: 99%

Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint

et al. 2000

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Brassinosteroids (BRs) are plant growth-promoting natural products required for plant growth and development. Physiological studies have demonstrated that exogenous BR, alone or in combination with auxin, enhance bending of the lamina joint of rice. However, little is known about the function of endogenous BR in rice or other grass species. We report here the phenotypical and molecular characterization of a rice dwarf mutant, d61 , that is less sensitive to BR compared to the wild type. We cloned a rice gene, OsBRI1 , with extensive sequence similarity to that of the Arabidopsis BRI gene, which encodes a putative BR receptor kinase. Linkage analysis showed that the OsBRI1 gene is closely linked to the d61 locus . Single nucleotide substitutions found at different sites of the d61 alleles would give rise to amino acid changes in the corresponding polypeptides. Furthermore, introduction of the entire OsBRI1 coding region, including the 5 Ј and 3 Ј flanking sequences, into d61 plants complemented the mutation to display the wild-type phenotype. Transgenic plants carrying the antisense strand of the OsBRI1 transcript showed similar or even more severe phenotypes than those of the d61 mutants. Our results show that OsBRI1 functions in various growth and developmental processes in rice, including (1) internode elongation, by inducing the formation of the intercalary meristem and the longitudinal elongation of internode cells; (2) bending of the lamina joint; and (3) skotomorphogenesis.

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Section: Internodes Differ In Their Sensitivity To Brsmentioning

confidence: 99%

Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint

et al. 2000

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“…They have molecular weights ranging from 450 to 500 Da, and are polyhydroxylated steroids. The bioactive members of the family also have a keto-or lactone function in ring B. Brassinosteroids have been identified in pollen from many species (Grove et al 1979;Schneider et al 1983;Yokota et al 1983;Plattner et al 1986;Ikekawa et al 1988;Takatsuto et al 1989;Gamoh et al 1990;Abe 1991), and bioassay data indicate their presence in an even wider range of plants (Mandava 1988).…”

Section: Introductionmentioning

confidence: 99%

Detection of brassinosteroids in pollen of Lolium perenne L. by immunocytochemistry

Taylor

Spuck

Smith

et al. 1993

Planta

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Abstract. Bioactive brassinosteroids have been localized in developing and mature pollen of anhydrously fixed rye-grass (Lolium perenne) by immunocytochemistry using polyclonal antibodies to castasterone generated in rabbits. Tricellular pollen fixed by freeze-substitution was also labelled in the starch granules. Study of the developmental sequence of the pollen through the microsporocyte, microspore, bicellular and tricellular stages showed that the brassinosteroids were increasingly sequestered in starch granules as the amyloplasts matured, supporting the view that these are storage organelles for these potent plant growth promoters. In bicellular pollen, heavy labelling was seen in the zone within 0.5 gm of the starch granule, where stromal tissue remains. Thus, the stroma may be the site of synthesis of these compounds. During aqueous fixation, the brassinosteroids leached from the starch granules of tricellular pollen, indicating that they would be quickly available after imbibition to influence the physiology of germinating pollen. The results from high-performance liquid chromatography of dansylaminophenylboronates from partially purified extracts of freshly dehisced tricellular pollen of rye-grass showed 25-methylcastasterone may be a minor component, together with two unknown peaks. No specific binding of brassinolide to any soluble proteins extracted from tricellular rye-grass pollen was observed using the antibodies in gel electrophoresis or enzyme-linked immunosorbent assays.

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“…An optimized set up with CI source and multiple reaction monitoring (MRM) after proper precursor ion selection allows the routine processing and analysis of up to 60 plant samples of between 20 and 300 mg of fresh weight per day [25]. The methods have been used for the analysis of most plant hormones, brassinosteroids, fatty acids, gibberellins and jasmonates after derivatization [9][10][11]21,[27][28][29]40,41,46,[53][54][55].…”

Section: Plant Hormone Analysis By Gc-msmentioning

confidence: 99%

Profiling of plant hormones by mass spectrometry

Pan

Wang

2009

Journal of Chromatography B

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Detection of Brassinolide and Castasterone in Alnus glutinosa (European Alder) Pollen by Mass Spectrometry/Mass Spectrometry

Cited by 24 publications

References 12 publications

Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint

Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint

Detection of brassinosteroids in pollen of Lolium perenne L. by immunocytochemistry

Profiling of plant hormones by mass spectrometry

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