Radio Frequency Identification (RFID) is a wireless technology used for tracking a tag attached to an object and uniquely identifying it. In the proposed work, an array antenna that has high gain, good impedance matching, good adaptation, and improved reflection coefficient (S 11 ) at 2.40 GHz for the detection system of objects or living things in motion, has been designed and fabricated. The Fire Resistant RT/duroid-5880 substrate is used for the design purpose of the proposed array antenna. The antenna is an array of four elements with an overall dimension of (223.9 × 98 × 1.56) mm 3 and has a microstrip line feed matched to 50 Ω. The impedance bandwidth achieved by the design is about 120 MHz (2.357-2.477 GHz) at the 2.40 GHz microwave band and has a reflection coefficient, S 11 of about −23.17 dB. The measured gain obtained is around 13 dB. An experimental study of the fabricated prototype of the optimized array antenna is performed and the results verify its good performance.