1α,25-dihydroxyvitamin D3 inhibits anti-CD40 plus IL-4-mediated IgE production in vitro

Heine, Guido; Anton, Katrin; Henz, Beate M.; Worm, Margitta

doi:10.1002/1521-4141(2002012)32:12<3395::aid-immu3395>3.0.co;2-i

Cited by 76 publications

(112 citation statements)

References 23 publications

(27 reference statements)

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“…Isolated peripheral blood mononuclear cells (4 Â 10 5 cells in 200 ml) were cultured in RPMI1640 containing 10% fetal calf serum as described (Heine et al, 2002), in 96-well round bottom culture plates in the presence or absence of 0.5 mg/ml S. enterotoxin B (Sigma) or 1% TT (Tetanus-Merieux, Sanofi Pasteur MSD, Strasbourg, France) in triplicates. After 24 h, the supernatants were stored at À80 1C until analysis using the Th1/Th2 cytokine CBA kit (BD), which allows the simultaneous quantification of secreted IL-2, IL-4, IL-5, IL-10, tumor necrosis factor-a (all sensitivity thresholds o3 pg/ml) and interferon-g (IFN-g; o8 pg/ml).…”

Section: Tt-induced Cytokine Releasementioning

confidence: 99%

“…Direct modulation of immune cells by vitamin D is most likely, as different immune cell types can produce and perceive calcitriol including monocytes, dendritic cells, T-helper cells and activated B cells (Hayes et al, 2003;Adorini and Penna, 2008). Previously, we demonstrated that activated human B cells can synthesize calcitriol from its precursor, which mediates enhanced expression of interleukin-10 (IL-10; Heine et al, 2008) and inhibited immunoglobulin E (IgE) production (Heine et al, 2002). In newborns, cord blood levels of 25-hydroxyvitamin D and the ratio of IL-10 to IgE are both higher in infants born in summer compared with winter (Zittermann et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Efficient tetanus toxoid immunization on vitamin D supplementation

et al. 2011

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Background/Objectives: Vitamin D mediates immunomodulatory functions, and its deficiency has been associated with an increased prevalence of immunological diseases. The supplementation of vitamin D might be therapeutically beneficial, for example, in lupus erythematosus patients. However, its affect on established recall immune responses is undefined. Subjects/Methods: In all, 32 individuals were randomized in a placebo controlled, double-blind setting, and received vitamin D (daily 2000 IU) for 10 weeks followed by tetanus toxoid (TT) booster immunization. Results: During vitamin D supplementation the median 25-hydroxyvitamin D serum concentration increased to 80.3 nM, which as expected decreased in the placebo group to 29.1 nM during the ultraviolet-deprived winter months. The TT-specific immunoglobulin G (IgG) boost efficiency was marginal higher in the vitamin D group (P ¼ 0.04). The increase of the 25-hydroxyvitamin D levels correlated with the increase of TT-IgG serum concentrations. The induction of specific serum IgA and specific antibody secreting cells was comparable between both groups. Accordingly, the TT-specific and polyclonally triggered T-cell cytokine profiles were stable as well. Conclusions: Vitamin D supplementation was successful and booster immunization induced efficiently specific antibodies titers.

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Section: Tt-induced Cytokine Releasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient tetanus toxoid immunization on vitamin D supplementation

et al. 2011

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“…After approval by the local ethics committee and informed consent was signed by the probands, B cells were isolated as described previously (19). Briefly, PBMC were isolated from buffy coats of healthy donors by density gradient separation (Ficoll hypaque, Biochrom) and B cells were purified using anti-CD19-coupled magnetic beads (Miltenyi Biotec) by magnetic cell sorting (Ն99.0% CD19 ϩ B cells, Ͻ0.5% CD3 ϩ T cells, and Ͻ0.5% CD14 ϩ monocytes, as assessed by flow cytometry).…”

Section: Cell Culturementioning

confidence: 99%

“…19. Briefly, anti-human IgE clone HP6061 coated on 96-well Maxisorb plates (Nunc) and biotinylated HP6029 (SouthernBiotech) were used and captured IgE was detected by the enzymatic activity of streptavidin-alkaline phosphatase (Zymed Laboratories) and the colorimetric analysis of the cleaved p-nitrophenyl phosphate substrate (Sigma-Aldrich) at 405 nm.…”

Section: Elisamentioning

confidence: 99%

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Liver X Receptors Control IgE Expression in B Cells

Heine

Dahten

Hilt

et al. 2009

The Journal of Immunology

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B lymphocytes play a fundamental role in the development of IgE-dependent allergic immune reactions. Upon appropriate activation, IgE class switch recombination is initiated in B cells, followed by terminal differentiation to IgE-secreting plasmablasts. This process is controlled by different nuclear receptors, including receptors for vitamin D, retinoids, and peroxisome proliferator-activated receptor-γ ligands. In this study, we show constitutive expression of the nuclear liver X receptor (LXR)α and LXRβ in peripheral human B cells. Activation of LXRs reduced secreted IgE (−68% ± 11) in CD40 and IL-4 activated B cells. The production of other isotypes, including IgG, IgM, IgA and B cell homeostatic parameters were not significantly altered by LXR activation. We identified inhibitory action of LXR activation on IgE production involving reduced phosphorylation of JNK and increased membrane CD23 expression (38% ± 11). The biological significance of our findings was validated by showing that systemic treatment of type I-sensitized BALB/c mice with LXR ligands reduced the serum concentrations of Ag-specific IgE in a dose-dependent manner (maximum, −52% ± 14). Thus, our data indicates that LXRs are involved in the control of IgE secretion by differentiating B cells.

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Autocrine IL‐10 promotes human B‐cell differentiation into IgM‐ or IgG‐secreting plasmablasts

et al. 2014

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B-cell-derived interleukin-10 (IL-10) is known to act in a paracrine fashion to suppress inflammation. Here, we show that IL-10 also acts in an autocrine manner to regulate the differentiation of activated human B cells. We report that IL-10 expression is not restricted to a dedicated B-cell subset, but is induced transiently in peripheral human naïve, memory, and CD5 + B cells alike upon activation. Global transcriptome comparison of activated human B cells, secreting IL-10 or not, identified 138 differentially regulated genes, most of which were associated with differentiation into antibody-secreting cells and reflecting autocrine IL-10 signaling. We monitored the differentiation of IL-10-secreting B cells and determined the effect of IL-10-blocking antibodies against its autocrine and paracrine signaling. IL-10 signaling promoted the differentiation of activated IL-10-secreting B cells into IgM-or IgG-secreting cells, but was dispensable for IgA secretion. Our data imply that B-cell-derived IL-10 not only suppresses immune reactions via paracrine mechanisms, but can also contribute to the differentiation of IL-10-secreting B cells into IgMand IgG-secreting plasmablasts through both autocrine and paracrine signaling.

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1α,25-dihydroxyvitamin D3 inhibits anti-CD40 plus IL-4-mediated IgE production in vitro

Cited by 76 publications

References 23 publications

Efficient tetanus toxoid immunization on vitamin D supplementation

Efficient tetanus toxoid immunization on vitamin D supplementation

Liver X Receptors Control IgE Expression in B Cells

Autocrine IL‐10 promotes human B‐cell differentiation into IgM‐ or IgG‐secreting plasmablasts

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