1H-NMR and MS Based Metabolomics Study of the Intervention Effect of Curcumin on Hyperlipidemia Mice Induced by High-Fat Diet

Abstract

Curcumin, a principle bioactive component of Curcuma longa L, is well known for its anti-hyperlipidemia effect. However, no holistic metabolic information of curcumin on hyperlipidemia models has been revealed, which may provide us an insight into the underlying mechanism. In the present work, NMR and MS based metabolomics was conducted to investigate the intervention effect of curcumin on hyperlipidemia mice induced by high-fat diet (HFD) feeding for 12 weeks. The HFD induced animals were orally administered with curcumin (40, 80 mg/kg) or lovastatin (30 mg/kg, positive control) once a day during the inducing period. Serum biochemistry assay of TC, TG, LDL-c, and HDL-c was conducted and proved that treatment of curcumin or lovastatin can significantly improve the lipid profiles. Subsequently, metabolomics analysis was carried out for urine samples. Orthogonal Partial Least Squares-Discriminant analysis (OPLS-DA) was employed to investigate the anti-hyperlipidemia effect of curcumin and to detect related potential biomarkers. Totally, 35 biomarkers were identified, including 31 by NMR and nine by MS (five by both). It turned out that curcumin treatment can partially recover the metabolism disorders induced by HFD, with the following metabolic pathways involved: TCA cycle, glycolysis and gluconeogenesis, synthesis of ketone bodies and cholesterol, ketogenesis of branched chain amino acid, choline metabolism, and fatty acid metabolism. Besides, NMR and MS based metabolomics proved to be powerful tools in investigating pharmacodynamics effect of natural products and underlying mechanisms.

Effect of high fat diet on NF‐кB microRNA146a negative feedback loop in ovalbumin‐sensitized rats

“…Also, the results of this study showed that there was a correlation between the expression level of miRNA-146a and study parameters such as EC 50 , maximum response, IL-1β, IRAK1, TRAF6, NF-kB, triglyceride, and HDL. In DIO groups, serum levels of TG, TC, LDL, and HDL were remarkably increased compared with those in the normal diet groups, indicating the occurrence of hyperlipidemia, which was similar to previous studies [45,46]. Given that it has been shown that free fatty acids can activate inflammatory pathways in tissues [7], the authors do not rule out the effect of lipid profiles in alteration of cellular pathways.…”

Recent Advances in NMR-Based Metabolomics

“…Another NMR-and MS-based study investigated the intervention effect of curcumin on hyperlipidemic mice induced by a high-fat diet. 88 While the serum biochemical assay indicated that treatment of curcumin improves the lipid profiles, metabolic pathways derived from urine metabolic profiles provided more detail and indicated reversal of the metabolic disorder.…”

1H NMR and MS based metabolomics study of the therapeutic effect of Cortex Fraxini on hyperuricemic rats

“…3-5 respectively. The identification of splitting and chemical shift of metabolites was accomplished by reference to the relevant literature (Fan 1996;Bollard et al 2000;Li et al 2015), and the resulting identification of metabolites is shown in S3 Table (a, b, c). The metabolomics results are presented using the OPLS-DA model (Fig.…”