1H NMR and MS based metabolomics study of the therapeutic effect of Cortex Fraxini on hyperuricemic rats

Wang, Yinan; Zhao, Min; Yi, Xin; Li, Jiajia; Wang, Miao

doi:10.1016/j.jep.2016.03.043

Cited by 49 publications

(34 citation statements)

References 52 publications

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“…Rats were randomly divided into a control group and a model group ( n = 6 rats/group). The treatment of rats in the model group before administration were in accordance with the previous studies we have reported (Wang et al, ). The model group were fed a high‐purine diet (oteracil potassium 100 mg/kg/day, yeast extract 10 g/kg/day and adenine 200 mg/kg/day) for 4 weeks.…”

Section: Methodssupporting

confidence: 82%

Comparative pharmacokinetic study of the main components of cortex fraxini after oral administration in normal and hyperuricemic rats

Wang

Zhao

Hao

et al. 2017

Biomedical Chromatography

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Cortex Fraxini is an important traditional Chinese herbal medicine used for the treatment of gout and hyperuricemia. An efficient and rapid ultra-performance liquid chromatography mass spectrometry method was developed and validated for simultaneous quantitation of six coumarins (aesculin, fraxin, aesculetin, fraxetin, sopoletin and 7-hydroxycoumarin) in normal and hyperuricemic rats plasma after oral administration of Cortex Fraxini. The method could successfully be applied for pharmacokinetics studies. The pharmacokinetic behavior of six coumarins in normal and hyperuricemia rats plasma was determined. Results showed that, for some of analytes, the pharmacokinetic parameters (AUC , AUC , C , T and CL) were significantly different between normal and hyperuricemic rats. The different pharmacokinetic parameters might result from renal impairment or a change of metabolic enzymes in the pathological state. The pharmacokinetic study in pathological state could provide more useful information to guide the clinical use of traditional Chinese herbal medicine.

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Section: Methodssupporting

confidence: 82%

Comparative pharmacokinetic study of the main components of cortex fraxini after oral administration in normal and hyperuricemic rats

Wang

Zhao

Hao

et al. 2017

Biomedical Chromatography

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“…Hyperuricemia is characterized by elevated blood uric acid levels [ 1 ], which cause accumulation of urate crystals in joints and the kidney, leading to gout and gouty arthritis [ 2 , 3 ]. The prevalence of hyperuricemia and gout is increasing worldwide, and uric acid is a risk factor of fatty liver, insulin resistance, hypertension, and cardiovascular diseases [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

DKB114, A Mixture of Chrysanthemum Indicum Linne Flower and Cinnamomum Cassia (L.) J. Presl Bark Extracts, Improves Hyperuricemia through Inhibition of Xanthine Oxidase Activity and Increasing Urine Excretion

et al. 2018

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Chrysanthemum indicum Linne flower (CF) and Cinnamomum cassia (L.) J. Presl bark (CB) extracts have been used as the main ingredients in several prescriptions to treat the hyperuricemia and gout in traditional medicine. In the present study, we investigated the antihyperuricemic effects of DKB114, a CF, and CB mixture, and the underlying mechanisms in vitro and in vivo. DKB114 markedly reduced serum uric acid levels in normal rats and rats with PO-induced hyperuricemia, while increasing renal uric acid excretion. Furthermore, it inhibited the activity of xanthine oxidase (XOD) in vitro and in the liver in addition to reducing hepatic uric acid production. DKB114 decreased cellular uric acid uptake in oocytes and HEK293 cells expressing human urate transporter (hURAT)1 and decreased the protein expression levels of urate transporters, URAT1, and glucose transporter, GLUT9, associated with the reabsorption of uric acid in the kidney. DKB114 exerts antihyperuricemic effects and uricosuric effects, which are accompanied, partially, by a reduction in the production of uric acid and promotion of uric acid excretion via the inhibition of XOD activity and reabsorption of uric acid. Therefore, it may have potential as a treatment for hyperuricemia and gout.

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“…NMR spectra of plasma (a), liver (b) and kidney (c) from the four groups are shown in Figure . The splitting and chemical shift of metabolites were identified by referring to the relevant literature (Chen et al, ; Dong et al, ; He et al, ; Wang et al, ). OPLS‐DA, the most commonly used supervised algorithm in metabolomic analysis, was used to deal with the metabolomics data.…”

Section: Resultsmentioning

confidence: 99%

Metabolomics analysis of the therapeutic mechanism of Semen Descurainiae Oil on hyperlipidemia rats using ¹H‐NMR and LC–MS

Cai

Zhao

Guan

et al. 2019

Biomedical Chromatography

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Semen descurainiae oil (SDO) is an important traditional Chinese medicine that was recently discovered to have the function of reducing blood lipids. Metabolomics analyses of plasma, liver and kidney in rats were performed using 1 H-NMR and LC-MS to illuminate the lower blood lipid concentration effect of SDO, and niacin was considered as the active control. The measure of total cholesterol (TC) and low-densitylipoprotein cholesterol (LDL-C) in plasma showed that SDO treatment decreased significantly the content of TC and LDL-C. An orthogonal partial least squaresdiscriminant analysis approach was applied to identify the different metabolic profiles of plasma, liver and kidney in rats and to detect related potential biomarkers. The results suggested that the metabolic profiles of the control group and hyperlipidemia group showed significant difference and the SDO and niacin group had effective antihyperlipidemia function. The biomarkers primarily concern lipid metabolism, amino acid metabolism and glycometabolism, and the change in biomarkers indicated that hyperlipidemia could cause the unbalance of these metabolic pathways in vivo. SDO reduced blood lipids by repairing amino acid and lipid metabolism. KEYWORDS 1 H NMR, hyperlipidemia, LC-MS, metabolomics, semen descurainiae oil

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1H NMR and MS based metabolomics study of the therapeutic effect of Cortex Fraxini on hyperuricemic rats

Cited by 49 publications

References 52 publications

Comparative pharmacokinetic study of the main components of cortex fraxini after oral administration in normal and hyperuricemic rats

Comparative pharmacokinetic study of the main components of cortex fraxini after oral administration in normal and hyperuricemic rats

DKB114, A Mixture of Chrysanthemum Indicum Linne Flower and Cinnamomum Cassia (L.) J. Presl Bark Extracts, Improves Hyperuricemia through Inhibition of Xanthine Oxidase Activity and Increasing Urine Excretion

Metabolomics analysis of the therapeutic mechanism of Semen Descurainiae Oil on hyperlipidemia rats using ¹H‐NMR and LC–MS

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1H NMR and MS based metabolomics study of the therapeutic effect of Cortex Fraxini on hyperuricemic rats

Cited by 49 publications

References 52 publications

Comparative pharmacokinetic study of the main components of cortex fraxini after oral administration in normal and hyperuricemic rats

Comparative pharmacokinetic study of the main components of cortex fraxini after oral administration in normal and hyperuricemic rats

DKB114, A Mixture of Chrysanthemum Indicum Linne Flower and Cinnamomum Cassia (L.) J. Presl Bark Extracts, Improves Hyperuricemia through Inhibition of Xanthine Oxidase Activity and Increasing Urine Excretion

Metabolomics analysis of the therapeutic mechanism of Semen Descurainiae Oil on hyperlipidemia rats using 1H‐NMR and LC–MS

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Metabolomics analysis of the therapeutic mechanism of Semen Descurainiae Oil on hyperlipidemia rats using ¹H‐NMR and LC–MS