1H and31P magnetization transfer studies of hindleg muscle in wild-type and creatine kinase-deficient mice

Kruiskamp, Marijn J.; Vliet, Gerard van; Nicolay, Klaas

doi:10.1002/(sici)1522-2594(200005)43:5<657::aid-mrm7>3.0.co;2-1

Cited by 22 publications

(9 citation statements)

References 19 publications

(21 reference statements)

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“…The echo time was 68, 136 or 272 ms. The magnetization transfer effect on Cr/PCr was subsequently confirmed in various studies in rat brain [17][18][19]44 and skeletal muscle, [45][46][47][48][49] and in human brain. [50][51][52] Various mechanisms may account for the observed MT effect:…”

Section: In Vivo Applicationsmentioning

confidence: 86%

“…However, Kruiskamp et al. 46 demonstrated that CK-deficient mice show almost unaffected MT effects in the absence of mitochondrial and cytoplasmic CK in skeletal muscle. This again indicates that Cr/PCr MT effects in proton NMR depend on a different process compared to the 31 P-MT effect of the CK reaction.…”

Section: Intermolecular Cross Relaxation Most Likely Accountsmentioning

confidence: 99%

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Magnetization transfer MRS

Leibfritz¹,

Dreher²

2001

NMR in Biomedicine

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This review deals with magnetization transfer (MT) effects observed in in vivo NMR spectroscopy. The basic experimental methods of MT experiments, the underlying kinetic mechanisms as well as the evaluation of measured data by fits to two-or three-pool models are described. Experimental results of both 31 P and 1 H in vivo MRS are reviewed showing the potential of MT experiments to characterize kinetic equilibrium reactions. This includes reactions where all involved components are MR visible, as well as situations where one indirectly measures pools of bound spins which cannot directly be observed in vivo. In particular, MT effects are described which have been observed in in vivo 1 H NMR spectra measured on the animal or human brain or on skeletal muscle. Possible mechanisms for the strong MT effects observed for the signals of creatine/phosphocreatine, lactate, alcohol and other metabolites are discussed. It is also emphasized that MT effects caused by water suppression techniques may lead to systematic errors in the quantification of in vivo 1 H NMR spectra.

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Section: In Vivo Applicationsmentioning

confidence: 86%

Section: Intermolecular Cross Relaxation Most Likely Accountsmentioning

confidence: 99%

Magnetization transfer MRS

Leibfritz¹,

Dreher²

2001

NMR in Biomedicine

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“…44 This suggests that a potential binding to the CK molecule is not the major factor for MT build-up. This would be in agreement with the observation that dipolar coupling of the creatine methylenes is also similar in wild-type and CK-deficient mice.…”

Section: Magnetization Transfer (Mt) In Mr Spectra Of Skeletal Musclesmentioning

confidence: 98%

“…On the other hand, it contains additional information about microscopic structures and mobility of metabolites. An additional problem is raised by specific MR techniques such as water suppression, where magnetization transfer effects [42][43][44][45][46] can reduce signal intensity depending on the sequence used. [47][48][49] …”

Section: Visibility Of Creatine/phosphocreatinementioning

confidence: 99%

“…[64][65][66] Magnetization transfer indicates that there is a motionally restricted proton pool in spin-exchange with free Cr/PCr. [42][43][44] Since MT effects on the Cr/PCr methyl groups do not change after death in an animal study, 42 it is concluded that both creatine and phosphocreatine contribute to the MT effect.…”

Section: Magnetization Transfer (Mt) In Mr Spectra Of Skeletal Musclesmentioning

confidence: 99%

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Dipolar coupling and ordering effects observed in magnetic resonance spectra of skeletal muscle

Boesch

Kreis

2001

NMR in Biomedicine

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Skeletal muscle is a biological structure with a high degree of organization at different spatial levels. This order influences magnetic resonance (MR) in vivo-in particular 1 H-spectra-by a series of effects that have very distinct physical sources and biomedical applications: (a) bulk fat (extramyocellular lipids, EMCL) along fasciae forms macroscopic plates, changing the susceptibility within these structures compared to the spherical droplets that contain intra-myocellular lipids (IMCL); this effect leads to a separation of the signals from EMCL and IMCL; (b) dipolar coupling effects due to anisotropic motional averaging have been shown for 1 H-resonances of creatine, taurine, and lactate; (c) aromatic protons of carnosine show orientation-dependent effects that can be explained by dipolar coupling, chemical shift anisotropy or by relaxation anisotropy; (d) limited rotational freedom and/or compartmentation may explain differences of 1 H-MR-visibility of the creatine/phosphocreatine resonances; (e) lactate 1 H-MR resonances are reported to reveal information on tissue compartmentation; (f) transverse relaxation of water and metabolites show multiple components, indicative of intra-, extracellular and/or macromolecular-bound pools, and in addition dipolar or J-coupling lead to a modulation of the signal decay, hindering straightforward interpretation; (g) diffusion weighted 31 P-MRS has shown restricted diffusion of phosphocreatine; (h) magnetization transfer (MT) indicates that there is a motionally restricted proton pool in spin-exchange with free creatine; reduced availability or restricted motion of creatine is particularly important for an estimation of ADP from 31 P-MR spectra, and in addition MT effects may alter the signal intensity of creatine 1 H-resonances following water-suppression pulses; (i) transcytolemmal water-exchange can be studied in 1 H-MRS by contrast-agents applied to the extracellular space; (k) transport of glucose across the cell membrane has been studied in diabetes patients using a combination of 13 C-and 31 P-MRS; and (l) residual quadrupolar interaction in 23 Na MR spectra from human skeletal muscle suggest that sodium ions are bound to ordered muscular structures.

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NMR Biomed.

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1H and31P magnetization transfer studies of hindleg muscle in wild-type and creatine kinase-deficient mice

Cited by 22 publications

References 19 publications

Magnetization transfer MRS

Magnetization transfer MRS

Dipolar coupling and ordering effects observed in magnetic resonance spectra of skeletal muscle

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