18F-Fluoride PET/CT and 99mTc-MDP SPECT/CT can detect bone cancer at early stage in rodents

Alves, Christiano Robles Rodrigues; Faria, Daniele de Paula; Carneiro, Camila de Godoi; Garcez, Alexandre Teles; Gutierrez, Vanessa Pacciari; Neves, Willian das; Almeida, Ney R. de; Cury, Yara; Chammas, Roger; Brum, Patrícia C.

doi:10.1016/j.lfs.2018.05.030

Cited by 3 publications

(2 citation statements)

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“…To test this hypothesis, we first applied histological analysis to validate that glycolytic fibers are more susceptible to atrophy than oxidative fibers in a rat model of severe cancer cachexia recently established by our group by injecting Wistar rats with Walker 256 tumor cells in the bone marrow via an osteotomy. 8,38 We next used OxICAT combined to gel-free mass-spectrometry analysis to investigate the cysteine Ox-PTMs in the proteome of both plantaris and soleus in tumor-bearing and control rats.…”

Section: Introductionmentioning

confidence: 99%

“…Here, we hypothesized that tumor progression induces cysteine Ox‐PTMs in the skeletal muscle, but these modifications would be different in muscles with the prevalence of glycolytic fibers when compared to muscles with the prevalence of oxidative fibers. To test this hypothesis, we first applied histological analysis to validate that glycolytic fibers are more susceptible to atrophy than oxidative fibers in a rat model of severe cancer cachexia recently established by our group by injecting Wistar rats with Walker 256 tumor cells in the bone marrow via an osteotomy 8,38 . We next used OxICAT combined to gel‐free mass‐spectrometry analysis to investigate the cysteine Ox‐PTMs in the proteome of both plantaris and soleus in tumor‐bearing and control rats.…”

Section: Introductionmentioning

confidence: 99%

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Cancer‐induced muscle atrophy is determined by intrinsic muscle oxidative capacity

et al. 2021

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We tested the hypothesis that cancer cachexia progression would induce oxidative post‐translational modifications (Ox‐PTMs) associated with skeletal muscle wasting, with different responses in muscles with the prevalence of glycolytic and oxidative fibers. We used cysteine‐specific isotopic coded affinity tags (OxICAT) and gel‐free mass spectrometry analysis to investigate the cysteine Ox‐PTMs profile in the proteome of both plantaris (glycolytic) and soleus (oxidative) muscles in tumor‐bearing and control rats. Histological analysis revealed muscle atrophy in type II fibers in plantaris muscle, with no changes in plantaris type I fibers and no differences in both soleus type I and II fibers in tumor‐bearing rats when compared to healthy controls. Tumor progression altered the Ox‐PTMs profile in both plantaris and soleus. However, pathway analysis including the differentially oxidized proteins revealed tricarboxylic acid cycle and oxidative phosphorylation as main affected pathways in plantaris muscle from tumor‐bearing rats, while the same analysis did not show main metabolic pathways affected in the soleus muscle. In addition, cancer progression affected several metabolic parameters such as ATP levels and markers of oxidative stress associated with muscle atrophy in plantaris muscle, but not in soleus. However, isolated soleus from tumor‐bearing rats had a reduced force production capacity when compared to controls. These novel findings demonstrate that tumor‐bearing rats have severe muscle atrophy exclusively in glycolytic fibers. Cancer progression is associated with cysteine Ox‐PTMs in the skeletal muscle, but these modifications affect different pathways in a glycolytic muscle compared to an oxidative muscle, indicating that intrinsic muscle oxidative capacity determines the response to cancer cachectic effects.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%