17α-Ethinylestradiol disrupts the ontogeny of the forebrain GnRH system and the expression of brain aromatase during early development of zebrafish

Vosges, Mélanie; Page, Yann Le; Chung, Bon Chu; Combarnous, Yves; Porcher, Jean‐Marc; Kah, Olivier; Brion, François

doi:10.1016/j.aquatox.2010.06.009

Cited by 81 publications

(45 citation statements)

References 71 publications

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“…The increase in fluorescence intensity of GnRH3 neural populations at 3 dpf could also reflect a proliferative action of BPA (Nanjappa et al, 2012) on GnRH3 neurons during early life stages, while the decrease in the fluorescence intensity of GnRH3 neurons at 5 dpf could be the result of an autocrine/ paracrine negative feedback system (Karigo and Oka, 2013) in response to the initial over-expression of GnRH3. Mechanisms underlying BPA-induced bidirectional changes in TN-GnRH3 neurons at the two development time points are not currently known; however, exposure-time sensitive actions of endocrine disrupting chemicals in developing brains have been reported in several studies (Peretz et al, 2014;Ramos et al, 2003;Vosges et al, 2010). For instance, chronic exposure to 17alpha-ethinylestradiol, a potent estrogenic compound, suppressed the size of the GnRH-immunoreactive soma in 10 dpf zebrafish (a closely related teleost) larvae, but not in 5, 20 and 30 dpf larvae (Vosges et al, 2010).…”

Section: Embryonic Bpa Exposure Alters Fluorescence Intensity Of Tngnmentioning

confidence: 98%

Low dose exposure to Bisphenol A alters development of gonadotropin-releasing hormone 3 neurons and larval locomotor behavior in Japanese Medaka

et al. 2016

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Section: Embryonic Bpa Exposure Alters Fluorescence Intensity Of Tngnmentioning

confidence: 98%

Low dose exposure to Bisphenol A alters development of gonadotropin-releasing hormone 3 neurons and larval locomotor behavior in Japanese Medaka

et al. 2016

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“…Studies dedicated to the impacts of EE2 in the brain of fish are very scarse. When larvae are exposed to EE2, the early development of the forebrain GnRH neurons circuitry is impaired with an increase in the number of GnRH neurons, a reduction in the size of their soma and a modification of their migration [134,135]. Taking advantage of the cyp19a1b-GFP transgenic zebrafish line (GFP expression restricted to RGCs and the strong susceptibility of the cyp19a1b promoter to estrogens), it has been shown that EE2 were approximately 50 times more potent than estradiol to induced fluorescence in RGCs of zebrafish larvae treated during 5 days [124].…”

Section: Adverse Effects Of Endocrine Disruptors During Early Neurogementioning

confidence: 99%

Steroid modulation of neurogenesis: Focus on radial glial cells in zebrafish

Pellegrini

Diotel

Vaillant-Capitaine

et al. 2016

The Journal of Steroid Biochemistry and Molecular Biology

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Estrogens are known as steroid hormones affecting the brain in many different ways and a wealth of data now document effects on neurogenesis. Estrogens are provided by the periphery but can also be locally produced within the brain itself due to local aromatization of circulating androgens. Adult neurogenesis is described in all vertebrate species examined so far, but comparative investigations have brought to light differences between vertebrate groups. In teleost fishes, the neurogenic activity is spectacular and adult stem cells maintain their mitogenic activity in many proliferative areas within the brain. Fish are also quite unique because brain aromatase expression is limited to radial glia cells, the progenitor cells of adult fish brain. The zebrafish has emerged as an interesting vertebrate model to elucidate the cellular and molecular mechanisms of adult neurogenesis, and notably its modulation by steroids. The main objective of this review is to summarize data related to the functional link between estrogens production in the brain and neurogenesis in fish. First, we will demonstrate that the brain of zebrafish is an endogenous source of steroids and is directly targeted by local and/or peripheral steroids. Then, we will present data demonstrating the progenitor nature of radial glial cells in the brain of adult fish. Next, we will emphasize the role of estrogens in constitutive neurogenesis and its potential contribution to the regenerative neurogenesis.Finally, the negative impacts on neurogenesis of synthetic hormones used in contraceptive pills production and released in the aquatic environment will be discussed.

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“…However, the mechanism of EE 2 in addition to metals on the disruption of HPLG axis is not clear. In this regard, increases of P450aromB transcript abundance have been reported in zebrafish (Danio rerio) juveniles exposed to EE 2 (Kazeto et al 2004, Vosges et al 2010 besides in the head of roach (Rutilus rutilus) at 28 and 56 days after spawning (Lange et al 2008). These findings indicate that metals and EE 2 could disrupt the HPLG axis by non specific mechanism.…”

Section: Discussionmentioning

confidence: 77%

DISRUPTION OF HYPOTHALAMUS-PITUITARY-LIVER-GONADS AXIS IN THE ENDANGERED Girardinichthys viviparus EXPOSED TO ENVIRONMENTALLY RELEVANT CONCENTRATIONS OF A MIXTURE OF METALS AND WITH 17α-ETHYNIL ESTRADIOL

Olivares-Rubio¹,

Dzul-Caamal²,

Nájera-Martínez³

et al. 2017

Rev. Int. Contam. Ambie.

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Girardinichthys viviparus is an endemic and endangered Mexican fish species with matrotrophic viviparity that only inhabits in some polluted water bodies in the Valley of Mexico. In the current study, G. viviparus of both sexes were exposed for 21 days to a mixture of metals with relevant environmental concentrations (T1) and to the same mixture spiked with 25 ng of 17α-ethynil estradiol (EE 2 )/L (T2). Some biomarkers involved in endocrine disruption of the hypothalamus-pituitary-liver-gonads axis such as gonadotropins I and II (GtH I and GtH II, respectively), and estradiol (E 2 ) concentrations in the head and gonads were measured. Vitellogenin (VTG) in the liver and gonads, and metallothionein (MT) in the head, liver and gonads were assessed. Increases of GtH I and decreases in GtH II, and alterations of E 2 in the head and gonads were found in fish treated with T1 and T2. Higher content of hepatic and gonadal VTG only in fish treated with T2 was detected. MT was notably induced by T2; however, a time-dependent MT reduction was observed. In both treatments, the hypothalamic-pituitary control point was most affected and their alterations were documented by gonadal and head content of E 2 . In female fish, it is most likely that endogenous levels of E 2 diminished the alterations elicited by EE 2 on this control point of the axis in contrast with male fish. The endocrine disruption of this fish species is a dynamic and complex process.Palabras clave: gonadotropinas, estradiol, vitelogenina, metalotioneínas, respuesta ligada al sexo RESUMENGirardinichthys viviparus es un pez mexicano endémico y en peligro de extinción con viviparidad matrotrófica que sólo habita en algunos cuerpos de agua contaminados del Valle de México. En este estudio, especimenes de G. viviparus de ambos sexos fueron expuestos durante 21 días a una mezcla de metales a concentraciones ambientalmente relevantes (T1) y a la misma mezcla enriquecida con 25 ng de 17α-etinil estradiol (EE 2 )/L (T2). En la cabeza y las gónadas se midieron biomarcadores implicados en el eje endocrino sexual hipotálamo-pituitaria-gónadas-hígado, así como las concentraciones de estradiol (E 2 ), gonadotropina I y II (GtH I y GtH II, respectivamente). En el Rev. Int. Contam. Ambie. 33 (2) 289-302, 2017 DOI: 10.20937/RICA.2017 H.F. Olivares-Rubio et al. 290hígado y las gónadas se evaluó la vitelogenina (VTG) y en la cabeza, el hígado y las gónadas los niveles de metalotioneínas (MT). En la cabeza y las gónadas de los peces expuestos a ambos tratamientos se observaron aumentos de la GtH I, disminuciones de GtH II y alteraciones de E 2 . Solamente en los peces expuestos al T2 se encontraron incrementos hepáticos y gonadales de VTG. Las MT fueron inducidas particularmente por el T2. Sin embargo, se observó una reducción dependiente del tiempo. En ambos tratamientos, el punto de control hipotálamo-pituitaria fue el más afectado y sus alteraciones fueron evidenciadas por los contenidos de E 2 . En contraste con los machos, en hembras es probable que los niveles e...

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17α-Ethinylestradiol disrupts the ontogeny of the forebrain GnRH system and the expression of brain aromatase during early development of zebrafish

Cited by 81 publications

References 71 publications

Low dose exposure to Bisphenol A alters development of gonadotropin-releasing hormone 3 neurons and larval locomotor behavior in Japanese Medaka

Low dose exposure to Bisphenol A alters development of gonadotropin-releasing hormone 3 neurons and larval locomotor behavior in Japanese Medaka

Steroid modulation of neurogenesis: Focus on radial glial cells in zebrafish

DISRUPTION OF HYPOTHALAMUS-PITUITARY-LIVER-GONADS AXIS IN THE ENDANGERED Girardinichthys viviparus EXPOSED TO ENVIRONMENTALLY RELEVANT CONCENTRATIONS OF A MIXTURE OF METALS AND WITH 17α-ETHYNIL ESTRADIOL

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