[17] Cloning and physical mapping of maize plastid genes

Bogorad, Lawrence; Gubbins, Earl J.; Krebbers, Enno; Larrinua, Ignacio M.; Mulligan, B. J.; Muskavitch, Karen M. T.; Orr, Elizabeth A.; Rodermel, Steven R.; Schantz, Rudi; Steinmetz, André; Vos, Guido De; Ye, Yukun K.

doi:10.1016/0076-6879(83)97160-4

Cited by 51 publications

(41 citation statements)

References 29 publications

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“…Total RNA was isolated from 100 hypocotyls (200 300 mg fresh weight) using the hot borate method described earlier [26]. Equal amounts of RNA (10 jzg) were separated on a 1.2% (w/v) agarose gel and transferred onto Nylon membranes (Amersham Hybond N+).…”

Section: Rna Isolation and Hybridizationmentioning

confidence: 99%

Is membrane potential involved in calmodulin gene expression after external stimulation in plants?

et al. 1996

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In Bidens pilosa (cv. radiata), a non-injurious stimulus induces a local and transient change in membrane potential, and an injurious stimulus induces a transmitted electrical signal described as the combination of an action potential and a slow wave. We have studied calmodulin gene expression after these stimuli. When the stimulus is non-injurious, calmodulin mRNA accumulation is only increased in the stimulated region. In contrast, when the stimulus is injurious, mRNA accumulation takes place in both wounded and distant, unwounded tissue. We propose that the slow wave plays a role in the long-distance transmission of a woundinduced information in plants.

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Section: Rna Isolation and Hybridizationmentioning

confidence: 99%

Is membrane potential involved in calmodulin gene expression after external stimulation in plants?

et al. 1996

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“…Those from fruits at the late development stages were extracted as described before [8]. RNAs were separated on 1.2% agarose-6% formaldehyde gels, transferred to Hybond-N+ membranes (Amersham).…”

Section: Northern Hybridizationsmentioning

confidence: 99%

Characterization and gene expression of an annexin during fruit development in Capsicum annuum

et al. 1996

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Several lines of evidence indicate that annexins, as calcium-dependent pbospbolipid-binding proteins, are involved in a variety of plant cellular processes. We were interested in determining if annexins are implicated in the highly regulated fruit development of bell pepper. By differential screening of several cDNA libraries, we isolated a full-length cDNA of 1180 bp encoding an annexin. Northern blot analyses show a differential expression pattern of the transcripts during the early stages of development and during ripening. Immunoblots using antiserum raised against p33/p35 from maize reveal that crossreactive polypeptides of about 30 kDa are present at each stage of fruit development in bell pepper. We partially purified the annexins from seedlings and green fruits. At least one annexin of 32 kDa is present in these plant tissues.

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“…RNA extraction was carried out as described (Bogorad et al, 1983). For northern blot analyses, the RNAs (10 pg) were separated by formaldehyde agarose gel electrophoresis (Maniatis et al, 1982) and blotted onto Hybond-N+ membranes (Amersham).…”

Section: Differential Gene Expression Analysismentioning

confidence: 99%

Fruit-Specific Expression of a Defensin-Type Gene Family in Bell Pepper (Upregulation during Ripening and upon Wounding)

et al. 1996

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We have isolated a 454-bp cDNA that encodes a nove1 fruit-specific defensin from bell pepper (Capsicum annuum). The encoded 75-amino-acid polypeptide contains an N-terminal domain characteristic of a signal peptide and a 48-amino-acid mature domain named 11. The mature protein, from which the N-terminal amino acid sequence was determined, contains eight cysteines that form four intramolecular disulfide bridges, suggesting a monomeric form for 11. In healthy fruits J1 is undetectable at the green stage but high levels accumulate during ripening. In wound areas of the green fruit the accumulation of 11 dramatically increased, suggesting a role for Jl in the plant's defense response. Moreover, we have demonstrated that 11 possesses an antifungal activity. We have isolated and characterized the corresponding two homologous genes (jl-1 and jl-2) that exist in the hell pepper genome. Both genes are interrupted hy the insertion, at the same position, of one intron of 853 bp for j7-7 and 4900 bp for j7-2. Northern blot and reverse transcriptase-polymerase chain reaction and restriction fragment length polymorphism analyses revealed that jl-7 transcripts are present only in fruits, only in trace amounts in mature green fruits, and that they accumulate to high levels in fully ripe fruits, whereas no jl-2 transcripts were detected in the samples monitored.

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[17] Cloning and physical mapping of maize plastid genes

Cited by 51 publications

References 29 publications

Is membrane potential involved in calmodulin gene expression after external stimulation in plants?

Is membrane potential involved in calmodulin gene expression after external stimulation in plants?

Characterization and gene expression of an annexin during fruit development in Capsicum annuum

Fruit-Specific Expression of a Defensin-Type Gene Family in Bell Pepper (Upregulation during Ripening and upon Wounding)

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