2012
DOI: 10.1590/s1517-83822012000100033
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16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting

Abstract: Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases. however, to our knowledge, this procedure has not been applied in vitreous samples from suspected endophthalmitis cases. Due to the hi… Show more

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Cited by 8 publications
(4 citation statements)
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References 22 publications
(14 reference statements)
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“…In preliminary PCR experiments 18 specimens showed a band in agarose gel in a correct fragment size, illustrating the presence of bacterial infection (56.4 %). Fungal infections may have caused endophthalmitis in 14 remaining PCR-negative samples [26]. BLAST analysis of PCR products indicated that the vitreous samples contained 12 bacterial species.…”
Section: Discussionmentioning
confidence: 99%
“…In preliminary PCR experiments 18 specimens showed a band in agarose gel in a correct fragment size, illustrating the presence of bacterial infection (56.4 %). Fungal infections may have caused endophthalmitis in 14 remaining PCR-negative samples [26]. BLAST analysis of PCR products indicated that the vitreous samples contained 12 bacterial species.…”
Section: Discussionmentioning
confidence: 99%
“…This emphasizes the need for molecular techniques to identify mixed pathogens with higher sensitivity. Very few studies have analyzed the polybacterial community, especially in POE cases (16,31). Hence, through this study, we investigated the polybacterial community of endophthalmitis cases through 16S rRNA gene library construction and sequencing.…”
Section: Discussionmentioning
confidence: 99%
“…16S gene expression was expectedly confirmed in all the tested bacterial strains. In the studies , the 16S rRNA gene for classification and identification of bacteria was used, while the suitability of this approach was discussed. The expression of the 16S rRNA gene demonstrated ribosome formation in cells, thus the presence of live bacterial cultures was found in our samples.…”
Section: Resultsmentioning
confidence: 99%