16S Ribosomal RNA (rDNA) Sequence Analysis and Phylogenetic Position of Sulfobacillus thermosulfidooxidans

Tourova, T. P.; Poltoraus, Andrey; Лебедева, И. А.; Цаплина, И. А.; Bogdanova, T. I.; Karavaiko, Gregory I.

doi:10.1016/s0723-2020(11)80069-x

Cited by 17 publications

(11 citation statements)

References 15 publications

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“…Isolates YTF1 (Frying Pan Hot Spring) and YS5-F1 (Sylvan Springs) most closely resembled Sulfobacillus strain ALV, a facultative, thermophilic iron oxidizer isolated from a coal spoil heap in Warwickshire, England (RDP similarity rank: score S~b 0.681 and 0.551, respectively). The sequences of heterotrophic isolates YTH1 and YTH2 (both from Frying Pan Hot Spring) most closely resembled sequence data (Tuorova et al, 1994) described for Sulfobacillus thermosulfidooxidans (S-ab 0.844 and 0.833, respectively). Phylogenetic relationships between these two Yellowstone isolates, the previously characterized moderately thermophilic and acidophilic heterotrophic bacteria Alicyclobacillus acidocaldarius and Alicyclobacillus acidoterrestris and the iron-oxidizer S. thermosulfidooxidans VKM B1269 are also shown in Fig.…”

Section: Alicyclobacillus Acidoterrestrissupporting

confidence: 68%

Thermophiles Biodiversity, Ecology, and Evolution

Reysenbach¹,

Voytek²,

Mancinelli³

2001

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xii Preface such enrichment procedures established the foundations of what we know of their diversity and physiology (Ramaley et al.; Johnson et al.). Recent advances in molecular phylogenetic techniques have eliminated the need to culture organisms to assess diversity. Studies based on these techniques have yielded a plethora of diverse, and, sometimes, novel lineages, such as the Korarchaeota (Graber et al.; Ferris et al.; Stoner et al.). Section III contains papers pertaining to the ecology and evolution of thermal spring microbial communities. These papers address aspects of the ecology of thermophiles and discuss how these organisms influence their environment through their physiological activity (Madigan et al.; Rothschild et al.). Thermal habitats can provide insights into unusual physiologies adapted for conditions similar to the early earth's environment. For example, many laminated stromatolite-like structures are found in the Archaean oceans, which are analogous to the structures formed by microbial mats in thermal springs. Findings from studies in these extant environments may aid interpretations of the nature, distribution, and paleoecology of ancient microorganisms (Lowe et al.; Ward et al.).The final section in the book addresses some of the applications and potential uses of thermophiles in industry, including the use of carotenoids as antioxidants in food and feed preparations, bioprocessing such as TNT degradation, and coal solubilization and desulfurization (Combie). Given the recent surge of interest in the biotechnological potential of thermophilic microorganisms, there is a need to clarify resource management policies so that microbial resources can be managed effectively to the benefit of all. The volume concludes with a discussion of ways in which the microbial organisms can be managed in areas such as national parks. Some of the specific issues regarding the management of Yellowstone's microbial resources are: inventory and monitoring of the resources, generation and maintenance of research support, habitat protection, legal and ecological ramifications of bioprospecting, and education (Varley et al.). These issues are of global concern and must eventually be confronted by all nations. Many of the initial discussions that started at the meeting in Yellowstone in 1995 were directed toward addressing these issues.

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Section: Alicyclobacillus Acidoterrestrissupporting

confidence: 68%

Thermophiles Biodiversity, Ecology, and Evolution

Reysenbach¹,

Voytek²,

Mancinelli³

2001

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“…However, phylogenetic analysis based on sequence comparisons of the 16S rRNA gene showed that species of the genus Alicyclobacillus belonged to a distinct line of descent within the low GjC Gram-positive lineage of the Bacteria that also included the closely related facultatively autotrophic species of the genus Sulfobacillus (Wisotzkey et al, 1992 ;Tourova et al, 1994 ; . The EMBL accession numbers for the 16S rRNA gene sequences of strains T and Alicyclobacillus acidoterrestris DSM 3922 T are AJ133633, AJ133632, AJ133634, AJ133635 and AJ133631, respectively.…”

Section: Introductionmentioning

confidence: 99%

Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores.

Albuquerque¹,

Rainey²,

Chung³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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Several acidophilic, slightly thermophilic or thermophilic Gram-positive isolates were recovered from solfataric soil at Furnas on the Island of Sa 4 o Miguel in the Azores. Phylogenetic analysis of the 16S rRNA gene sequence showed that these organisms represented two novel species of the genus Alicyclobacillus. Strains FR-11 T and FR-1b had an optimum growth temperature of about 50 SC, whereas strains FR-3 and FR-6 T had an optimum growth temperature of about 60 SC. Biochemical, physiological and chemotaxonomic characteristics did not distinguish isolates FR-3 and FR-6T from the type strain of Alicyclobacillus acidocaldarius ; however, strains FR-11 T and FR-1b could be easily distinguished from the type strain of Alicyclobacillus acidoterrestris by the carbon source assimilation pattern and the fatty acid composition. On the basis of the phylogenetic analysis, physiological and biochemical characteristics, and fatty acid composition the name Alicyclobacillus hesperidum is proposed for the species represented by strains FR-11 T and FR-1b ; a formal name for the new genomic species represented by strains FR-3 and FR-6 T is not proposed at this time.

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“…The presence of w-cyclohexane C,, and C,, was revealed in this new organism (29). The G + C contents were found to be 53 2 1 mol% and 54 2 1 mol%, respectively, for SD-11 and S. themzosuljidooxidans, and were identical at 59 2 1 mol% for their 16s rDNA sequences (35). However many physiological differences were noted between isolate SD-ll and Sulfobacillus sp.…”

Section: Discussionmentioning

confidence: 89%

“…Even if the genera Sulfobacillus and Alicyclobacillus possess a number of distinctive phenotypic features that could justify their separation into two genera (Table 7), phylogenetic analyses confirm their close proximity (35). Since A. cycloheptanicus is found to be more related to Sulfobacillus strains than to other Alicyclobacillus strains, and because both groups share growth factor requirements (Table 7), a revision of the taxonomic nomenclature of the group at the genus level may be necessary, but this will require the description of additional species.…”

Section: Discussionmentioning

confidence: 99%

“…The 14 conserved nucleotide positions reported by Tourova et al (39, which link the genera Alicyclobacillus and Sulfobacillus and differentiate them from the other low-percentage-G+ C grampositive groups were also found in isolate SD-11 (154-G, 1123-T, 1150-A, 1151-C, 1297-G, and 1298-T). However, the clade formed by strains ALV, BC, and C-MT1 was shown by parsimony and maximum-likelihood methods to be quite remote from the genera Sulfobacillus and Alicyclobacillus (22,35), even if the isolate BC has been described as a gramnegative rod-shaped bacterium (15,27).…”

Section: Discussionmentioning

confidence: 99%

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Sulfobacillus disulfidooxidans sp. nov., a New Acidophilic, Disulfide-Oxidizing, Gram-Positive, Spore-Forming Bacterium

Dufresne

Bousquet

Boissinot

et al. 1996

International Journal of Systematic Bacteriology

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An acidophilic, disulfide-oxidizing, mesophilic, aerobic bacterium was isolated from wastewater sludge. The new organism is a gram-positive sporulated rod. It can use elemental sulfur and pyrite as sole energy sources and grows on organic substrates such as glutamate and glucose. It also grows on the following organic sulfur substrates: oxidized and reduced glutathione, cysteine, cystine, and dithio(bis) benzothiazole and clearly shows a preference for disulfide bond-containing substrates. The optimal pH of growth is between 1.5 and 2.5, depending on the substrate used, and the growth temperature range varies from 4 to 40°C, with an optimal value at 35°C. The G+C chromosomal DNA content was measured at 53 A 1 mol%. Phylogenetic analysis of 16s genes coding for rRNA sequences places the new isolate in the genus Sulfobacillus. In addition, unique phenotypic and physiologic characteristics and DNA homology values assign the isolate to a new species in the genus. Therefore, this new isolate has been named Sulfobacillus disulJidooxidans and has been assigned ATCC number 51911.Many facultatively heterotrophic bacteria are known for their ability to grow under highly acidic conditions. Some of these within the genera Sulfolobus (5) and Acidianus (31) are extremely thermophilic archaebacteria. They obtain their energy by the oxidation or reduction of elemental sulfur or from organic substrates. Others are mesophilic gram-negative bacteria belonging to the genus Acidiphilium (2, 18) and are able to use organic substrates at pH 3.0 or belong to the genus Thiobacillus, such as Thiobacillus acidophilus (17), which is also known for its ability to grow with elemental sulfur or glucose at pH 2.5.However, the only acidophilic, facultatively autotrophic, gram-positive, spore-forming bacillus so far characterized is Sulfobacillus thermosu&dooxidans (16), together with two subspecies ("themzotolerans" and "asporogenes") that do not produce spores (19). Golovacheva and Karavaiko (16) first described the genus Sulfobacillus in 1978 as gram-positive, sporeforming bacteria growing on elemental sulfur, ferrous iron, and other metal sulfides. The rod-shaped cells were described as fairly pleomorphic. The spores were spherical or slightly oval, terminal, subterminal, or paracentral. S. therrnosulfdooxidans is a typical acidophilic, thermotolerant, and facultatively autotrophic bacterium. Since then, four isolates which are gramvariable, thermotolerant acidophiles have been recovered from mines: ALV (27), BC (27), C-MT1(15), and TH3 (4), and have been described as belonging presumably to this genus. Some other gram-positive bacteria from the genus Alicyclobacillus (7-9) can also grow under acidic conditions, but they are heterotrophic bacteria.This work reports on the isolation and characterization of a new facultatively autotrophic, mesophilic, acidophilic, grampositive, spore-forming bacterium (SD-11) that could belong to the genus Sulfobacillus. MATERIALS AND METHODSBacterial strains and culture conditions. The acidophilic strain SD-1...

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16S Ribosomal RNA (rDNA) Sequence Analysis and Phylogenetic Position of Sulfobacillus thermosulfidooxidans

Cited by 17 publications

References 15 publications

Thermophiles Biodiversity, Ecology, and Evolution

Thermophiles Biodiversity, Ecology, and Evolution

Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores.

Sulfobacillus disulfidooxidans sp. nov., a New Acidophilic, Disulfide-Oxidizing, Gram-Positive, Spore-Forming Bacterium

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