Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores.

Abstract

Several acidophilic, slightly thermophilic or thermophilic Gram-positive isolates were recovered from solfataric soil at Furnas on the Island of Sa 4 o Miguel in the Azores. Phylogenetic analysis of the 16S rRNA gene sequence showed that these organisms represented two novel species of the genus Alicyclobacillus. Strains FR-11 T and FR-1b had an optimum growth temperature of about 50 SC, whereas strains FR-3 and FR-6 T had an optimum growth temperature of about 60 SC. Biochemical, physiological and chemotaxonomic characteristics did not distinguish isolates FR-3 and FR-6T from the type strain of Alicyclobacillus acidocaldarius ; however, strains FR-11 T and FR-1b could be easily distinguished from the type strain of Alicyclobacillus acidoterrestris by the carbon source assimilation pattern and the fatty acid composition. On the basis of the phylogenetic analysis, physiological and biochemical characteristics, and fatty acid composition the name Alicyclobacillus hesperidum is proposed for the species represented by strains FR-11 T and FR-1b ; a formal name for the new genomic species represented by strains FR-3 and FR-6 T is not proposed at this time.

Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus

“…Simple v-cyclohexane acids were also identified from the published data (Oshima & Ariga, 1975) and oxyacids by specific fragmentation of Me-TMS derivatives (methyl-O-trimethylsilyl derivatives) and methyl esters (without silylation). It was shown that strain K1 T differed only slightly from A. acidocaldarius, A. acidoterrestris, Alicyclobacillus acidiphilus, Alicyclobacillus sendaiensis, Alicyclobacillus hesperidum (Wisotzkey et al, 1992;Goto et al, 2003;Matsubara et al, 2002;Tsuruoka et al, 2003;Albuquerque et al, 2000), S. disulfidooxidans (Dufresne et al, 1996) and strains of S. thermosulfidooxidans (Tsaplina et al, 1994). All these strains contained v-alicyclic (cyclohexanoic) fatty acids as the major lipid component.…”

“…Strain K1 T grew optimally at 37-42 u C, differing from the known thermophilic bacteria of the genus Alicyclobacillus, which are characterized by optimal growth at 42-60 uC and Dufresne et al, 1996); 3, A. acidocaldarius subsp. acidocaldarius DSM 446 T (Wisotzkey et al, 1992); 4, A. acidoterrestris DSM 3922 T (Wisotzkey et al, 1992); 5, A. cycloheptanicus DSM 4006 T (Wisotzkey et al, 1992); 6, A. hesperidum DSM 12489 T (Albuquerque et al, 2000); 7, A. acidiphilus TA-67 T ; 8, A. herbarius DSM 13609 T ; 9, A. pomorum DSM 14955 T (Goto et al, 2003); 10, A. sendaiensis ATCC BAA-609 T (Tsuruoka et al, 2003). +, Positive result; 2, negative result; W, weakly positive; ND, not determined.…”

Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beverages.

“…The possession of ω-cyclohexane fatty acid as the major acid distinguishes the isolate from A. cycloheptanicus and A. herbarius, which possess ω-cycloheptane fatty acid as the major acid. ω-Alicyclic acid is unique H. Matsubara and others Albuquerque et al (2000).…”

“…k j k j j Growth in 5 % NaCl k k j k k Growth with lysozyme present j k k  k Acid production from : Albuquerque et al (2000).…”

“…Alicyclobacillus (Wisotzkey et al, 1992). Recently, Alicyclobacillus hesperidum (Albuquerque et al, 2000), ' Alicyclobacillus mali ' and ' Alicyclobacillus acidocaldarius subsp. rittmannii ' (Nicolaus et al, 1998), which possess ω-cyclohexane fatty acid, and Alicyclobacillus herbarius (Goto et al, 2002), which possesses ω-cycloheptane fatty acid, have been reported.…”