16S rDNA Full-Length Assembly Sequencing Technology Analysis of Intestinal Microbiome in Polycystic Ovary Syndrome

Abstract

ObjectiveTo study the characteristics and relationship of the gut microbiota in patients with polycystic ovary syndrome (PCOS).MethodWe recruited 45 patients with PCOS and 37 healthy women from the Reproductive Department of Shengjing Hospital. We recorded their clinical indexes, and sequenced their fecal samples by 16S rDNA full-length assembly sequencing technology (16S-FAST).ResultWe found decreased α diversity and different abundances of a series of microbial species in patients with PCOS compared to healthy controls. We found LH and AMH were significantly increased in PCOS with Prevotella enterotype when compared to control women with Prevotella enterotype, while glucose and lipid metabolism level remained no significant difference, and situations were opposite in PCOS and control women with Bacteroides enterotype. Ruminococcus gnavus, Prevotella stercorea, Dialister succinatiphilus and Bacteroides fragilis were more abundant while Christensenellaceae spp. were less abundant in the PCOS group. P. stercorea was significantly more prevalent in PCOS-not insulin resistance (NIR) compared to control-NIR and PCOS-not overweight (NOW) patient groups compared to control-NOW groups. Kyoto Encyclopedia Genes and Genomes reflecting pathways related to lipopolysaccharide biosynthesis were more abundant in the PCOS group.ConclusionOur study found gut microbiota that had different abundance in patients with PCOS compared to healthy controls. An intimate relationship was shown between the gut microbiota and pathological changes in PCOS. We suggest the gut microbiota should be taken into consideration in the treatment of symptoms of PCOS via drugs and diet.

Gut and Vaginal Microbiomes in PCOS: Implications for Women’s Health

“…Further studies found that transplantation of fecal microbiota from women with PCOS would lead to the elevated proportion of disrupted ovarian functions, insulin resistance and infertility [15]. Also, some studies reported that Bacteroides were enriched in the PCOS women (35). Zhang et al found that the larger proportion of Bacteroides in PCOS women by analyzing the stool microbiome.…”

Interplay between PCOS and microbiome: The road less travelled

“…Prevotella might be one of the identifier in IR influenced healthy weight PCOS women and can be correlated with the PCOS disease as Prevotella is a prominent SCFA‐producing bacterium in the intestine, and it can also generate anti‐inflammatory compounds that can facilitate the development of regulatory T cells in the gut, thereby lowering inflammation 79 . Another finding reported that PCOS‐IR women have predominantly Bacteroidetes enterotype 80 . As a result of these altered microbiota, LPS containing microbes and steroid production pathways are enhanced in PCOS‐IR women 78 .…”

“…79 Another finding reported that PCOS-IR women have predominantly Bacteroidetes enterotype. 80 As a result of these altered microbiota, LPS containing microbes and steroid production pathways are enhanced in PCOS-IR women. 78 Inflammatory mediators can activate serine, resulting in enhanced phosphorylation, suppressed tyrosine kinase activity, and significantly reduced insulin-stimulated receptor tyrosine phosphorylation obstructing insulin receptor activation and downstream cascade activation thus, lowering IRS-1 function.…”

Oral-Gut Microbiome Analysis in Patients With Metabolic-Associated Fatty Liver Disease Having Different Tongue Image Feature

“…Next, data from electrophoresis and the measurements of Qubit concentrations were assembled for quality control before Illumina NovaSeq 6000 sequencing (Illumina, USA). The detailed process was reported in a previously published study ( Dong et al., 2021 ).…”