16S rDNA-based metagenomic analysis of human oral plaque microbiota in patients with atherosclerosis and healthy controls

Abstract

To address the question if an altered oral microbiota is associated with atherosclerosis. Twenty patients suffering from atherosclerosis and 10 controls were recruited. Clinical oral, medical and laboratory investigations were performed. Oral bacteria were collected and 16S rDNA was sequenced following Single strand conformation polymorphism.(SSCP) Probing pocket depths in patients were significantly elevated. The oral microbiota of patients and controls were dominated by Fusobacterium (16%/17%), Streptococcus (21%/14%), Prevotella (10%/12%), Enterococcus (12%/12%), Porphyromonas (8%/7%), TM7 (0%/7%) and Veillonella (6%/7%). Differences in diversity were not significant between groups. The pathology of atherosclerosis may not be related to significant qualitative changes of the oral microbiota.

A potential oral microbiome signature associated with coronary artery disease in Tunisia

“…For these genera, no significant difference was found between both groups. These bacterial genera were also reported with the highest relative abundances in the oral microbiota of coronary patients and controls in the previous studies [ 12 , 13 ]. Streptococcus and Veillonella are early colonizers during the process of biofilm formation in the oral cavity initiated by the aggregation of these bacteria to establish an initial microenvironment [ 8 ].…”

“…In the previous studies, while abundance of Anaeroglobus in the oral cavity was associated with symptomatic atherosclerosis [ 12 ], CAD was not related to significant qualitative changes of the oral microbiota compared with healthy controls in other studies [ 11 , 13 , 14 ].…”

“…To address the question if an altered oral microbiota is associated with atherosclerosis compared with healthy subjects, 16S ribosomal RNA (rRNA)-based metagenomic analysis was conducted in several studies for different study populations: in Sweden [ 11 , 12 ], Germany [ 13 ], and India [ 14 ]. Abundance of Anaeroglobus in the oral cavity was associated with symptomatic atherosclerosis [ 12 ].…”

Oral microbiome-systemic link studies: perspectives on current limitations and future artificial intelligence-based approaches

“…Considering the potential role of inflammation during periodontitis in escalating artherosclerosis, it is important to obtain the oral health status of subjects while deciphering this link. None of the studies, except Ismail et al (2012), reported oral health data to stratify the population into study or control groups. Moreover, one of the studies has not taken oral samples and inferences were drawn based solely on the identification of the taxa in the artherosclerotic plaque (Calandrini et al 2014).…”