16S partial gene mitochondrial DNA and internal transcribed spacers ribosomal DNA as differential markers of Trichuris discolor populations

Callejón, Rocío; Halajian, Ali; Rojas, Manuel de; Marrugal, Ángela; Guevara, Diego; Cutillas, Cristina

doi:10.1016/j.vetpar.2011.11.033

Cited by 29 publications

(26 citation statements)

References 26 publications

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“…Two major clades were revealed within enoplid nematodes: (Mermithidae + Longidoridae) and (Trichuridae + Trichinellidae). Within Trichuridae, T. ovis and T. discolor were revealed as two distinct groups in the branch with high support value (1.00 posterior probability for BI and 100% bootstrap values for ML and MP analyses) using the amino acid dataset, supporting the results of the recent study using ITS rDNA and a portion of the mt DNA sequences dataset as genetic markers (Callejón et al, 2012).…”

Section: Phylogenetic Analysessupporting

confidence: 82%

“…Adult samples representing T. discolor were obtained from the caecum of wild yak during surgery in Noel Cover Grassland in Sichuan Province, China. The whipworms were washed in physiological saline, identified preliminarily to species based on host preference, morphological characters and predilection sites (Cutillas et al, 1995;Callejón et al, 2012), fixed in 70% (v/v) ethanol and stored at À20°C until use.…”

Section: Parasites and Dna Extractionmentioning

confidence: 99%

“…T. ovis and T. discolor are not considered to be host specific because they are often found in the caecum and colon of bovines, sheep and goats. A morphological study revealed differences between T. ovis and T. discolor isolated from bovines (Callejón et al, 2012). A molecular study, using the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA (rDNA) and a portion of large subunit ribosomal RNA (16S) gene of the mitochondrial (mt) genome as genetic markers, supported that T. ovis and T. discolor represent two distinct species (Callejón et al, 2012).…”

Section: Introductionmentioning

confidence: 98%

“…A morphological study revealed differences between T. ovis and T. discolor isolated from bovines (Callejón et al, 2012). A molecular study, using the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA (rDNA) and a portion of large subunit ribosomal RNA (16S) gene of the mitochondrial (mt) genome as genetic markers, supported that T. ovis and T. discolor represent two distinct species (Callejón et al, 2012). However, ITS-1 and ITS-2 rDNA sequences can be difficult or unsuitable to provide reliable genetic marker for systematic studies of enoplid nematodes because significant level of size and sequence polymorphism (heterogeneity) exists in these rDNA regions within individual nematodes (Gasser, 1997;Gasser et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae)

Wang

et al. 2012

Infection, Genetics and Evolution

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Section: Phylogenetic Analysessupporting

confidence: 82%

Section: Parasites and Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae)

Wang

et al. 2012

Infection, Genetics and Evolution

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“…The Clade subdivision based on host nutritional requirements suggested by Callejón et al (2012) does not completely explain independent clustering into two phylogenetic lineages of Trichuris sp. from human and non-human primates and of T. suis, but it probably reflects ancient association between human and pigs as a consequence of domestication.…”

Section: Discussionmentioning

confidence: 88%

Genetic heterogeneity and phylogeny of Trichuris spp. from captive non-human primates based on ribosomal DNA sequence data

Cavallero

Liberato

Friedrich

et al. 2015

Infection, Genetics and Evolution

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a b s t r a c tNematodes of the genus Trichuris, known as whipworms, are recognized to infect numerous mammalian species including humans and non-human primates. Several Trichuris spp. have been described and species designation/identification is traditionally based on host-affiliation, although cross-infection and hybridization events may complicate species boundaries. The main aims of the present study were to genetically characterize adult Trichuris specimens from captive Japanese macaques (Macaca fuscata) and grivets (Chlorocebus aethiops), using the ribosomal DNA (ITS) as molecular marker and to investigate the phylogeny and the extent of genetic variation also by comparison with data on isolates from other humans, non-human primates and other hosts. The phylogenetic analysis of Trichuris sequences from M. fuscata and C. aethiops provided evidences of distinct clades and subclades thus advocating the existence of additional separated taxa. Neighbor Joining and Bayesian trees suggest that specimens from M. fuscata may be distinct from, but related to Trichuris trichiura, while a close relationship is suggested between the subclade formed by the specimens from C. aethiops and the subclade formed by T. suis.The tendency to associate Trichuris sp. to host species can lead to misleading taxonomic interpretations (i.e. whipworms found in primates are identified as T. trichiura). The results here obtained confirm previous evidences suggesting the existence of Trichuris spp. other than T. trichiura infecting non-human living primates.

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The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of ribosomal DNA

et al. 2012

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Trichuris nematodes were isolated from roe deer (Capreolus capreolus). At first, nematodes were determined using morphological and biometrical methods. Subsequently genomic DNA was isolated and the ITS1-5.8S-ITS2 segment from ribosomal DNA (RNA) was amplified and sequenced using PCR techniques. With u sing morphological and biometrical methods, female nematodes were identified as Trichuris globulosa, and the only male was identified as Trichuris ovis. The females were classified into four morphotypes. However, analysis of the internal transcribed spacers (ITS1-5.8S-ITS2) of specimens did not confirm this classification. Moreover, the female individuals morphologically determined as T. globulosa were molecularly identified as Trichuris discolor. In the case of the only male molecular analysis match the result of the molecular identification. Furthermore, a comparative phylogenetic study was carried out with the ITS1 and ITS2 sequences of the Trichuris species from various hosts. A comparison of biometric information from T. discolor individuals from this study was also conducted.

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16S partial gene mitochondrial DNA and internal transcribed spacers ribosomal DNA as differential markers of Trichuris discolor populations

Cited by 29 publications

References 26 publications

Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae)

Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae)

Genetic heterogeneity and phylogeny of Trichuris spp. from captive non-human primates based on ribosomal DNA sequence data

The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of ribosomal DNA

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