16 S rRNA gene diversity and gut microbial composition of the Indian white shrimp (Penaeus indicus)

Patil, Prasanna Kumar; Vinay, T.N.; Ghate, Sudeep D.; Baskaran, Viswanathan; Avunje, Satheesha

doi:10.1007/s10482-021-01658-9

Cited by 12 publications

(3 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies have reported the formation of microbiota, which get stabilized by post larval stages (PL5-PL15), indicating the role of early feeding, food and feeding habits, feed additives in shaping the gut microbiome in penaeid shrimp P. monodon [9] and P. vannamei [12]. Recently, we have reported the gut microbial composition in adult P. indicus obtained from wild and farmed environments [13]. However, information on the formation of microbiota in the early developmental stages of Indian white shrimp, P. indicus is not available.…”

Section: Introductionmentioning

confidence: 94%

Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus

et al. 2022

Self Cite

View full text Add to dashboard Cite

Gut microbiota is known to in uence the physiology, health, nutrient absorption, reproduction, and other metabolic activities of aquatic organisms. Microbial composition can in uence intestinal immunity and are considered as health indicators. Information on gut microbial composition provides potential application possibilities to improve shrimp health and production. In the absence of such information for Penaeus indicus, the present study reports the microbial community structure associated with its early developmental stages. Bacterial community associated with the early developmental stages (egg, nauplii, zoea, mysis, postlarvae-1, postlarvae-6 and postlarvae-12) from two hatchery cycles were analysed employing 16S rRNA high throughput sequencing. Proteobacteria and Bacteroidetes, were the two dominant phyla in P. indicus development stages. Sequential sampling revealed the constant change in the bacterial composition at genus level. Alteromonas was dominant in egg and nauplii stage, whilst Ascidiaceihabitans (formerly Roseobacter) was the dominant genera in both PL6 and PL12. The bacterial composition was highly dynamic in early stages and our study suggests that the mysis stage is the critical phase in transforming the microbial composition and it gets stabalised by early post larval stages. This is the rst report on the composition of microbiota in early developmental stages of P. indicus. Based on these results the formation of microbial composition seems to be in uenced by feeding at early stages. The study provides valuable information to device intervention strategies for healthy seed production.

show abstract

Section: Introductionmentioning

confidence: 94%

Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Diversity differences in the intestinal microbiota have been reported in a number of shrimp species, such as Indian white shrimp (Penaeus indicus), Pacific white shrimp (Litopenaeus vannamei), red claw crayfish (Cherax quadricarinatus) and red swamp crayfish (Procambarus clarkii) [28][29][30]. The intestinal microbiota of Indian white shrimp differed among different stages and showed decreasing diversity with age.…”

Section: Discussionmentioning

confidence: 99%

The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (Macrobrachium rosenbergii)

Xu,

Ren,

et al. 2023

Fishes

View full text Add to dashboard Cite

Intestinal bacterial communities play an important role in the growth and health of aquatic animal hosts and have drawn increasing attention. However, the role of the intestinal microbiota in the growth of freshwater prawns remains unclear. Here, the intestinal microbiota of freshwater prawns (Macrobrachium rosenbergii) at different life stages (one, two, and three months old) were investigated using 16S rRNA sequencing. The results showed that community richness and diversity increased with growth, which might be one of the reasons that the prawns maintained a fast growth rate before sexual maturation. Three core phyla were identified in the one-month-old group, namely, Firmicutes (79.24%), Proteobacteria (17.09%) and Actinobacteriota (2.01%). Five core phyla were identified in the two-month-old group, including Firmicutes (47.84%), Proteobacteria (44.22%), Actinobacteriota (1.83%), Acidobacteriota (1.66%) and Bacteroidota (1.24%), and the core phyla in the three-month-old group were similar, except for Acidobacterota, which was not identified. A total of 12 core genera were identified in all samples, and significant differences were observed in the relative abundance of gut microbiota between the three groups (p < 0.05). Exiguobacterium, Lactococcus and Shewanella were the three most significantly differentially abundant genera between stages. In addition, Candidatus Hepatoplasma was detected only in the two- and three-month-old prawn groups. This study provides information on the differences in the intestinal microbiota in different developmental stages, which contribute to adaptation to salinity in the early developmental stage and digestive ability to meet the growth needs of Macrobrachium rosenbergii.

show abstract

“…The raw data was optimized by removing the low-quality sequences using the QIIME (version 1.17) software, and chimeric sequences were identi ed and deleted using the UCHIME algorithm (Patil et al 2021). The re ned sequences were clustered to the operational taxonomic units (OTUs) by 97% similarity using UPARSE (Version 7.1), and the OTUs was assigned according to the SILVA database with 70% con dence threshold (Pavlovska et al 2021).…”

Section: Statistical Analysis Of Datamentioning

confidence: 99%

Exploratory study on interaction between bacteria and fungi from mulberry to intestine of Bombyx mori

Xiang

et al. 2022

Preprint

View full text Add to dashboard Cite

Many kinds of antibiotics in the environments deeply affect the microbial composition, but their effect on the interactions among the host microbiota is still poorly understood. This study used Bombyx mori as the model organism by feeding the antibiotics-treated mulberry leaf, preliminarily explored the effects of antibiotic exposure on the interaction of bacteria and fungi in the worm’s intestine. Our results showed that the elimination of fungi significantly reduced the bacterial richness and diversity in the worm’s intestine after exposure of anti-fungal amphotericin B, while the elimination of bacteria dramatically increased the richness and diversity of fungi after exposure of anti-bacterial ampicillin-streptomycin. The results revealed the intestinal bacteria and fungi of Bombyx mori were markedly shaped by the mulberry leaf flora. Anti-bacterial antibiotics enhanced the correlation between the host gut fungi and the diet-deriving fungi, while anti-fungal antibiotics weakened the correlation between the host gut bacteria and the diet-deriving bacteria. The data preliminarily established a simple model to explore the effect of antibiotics on the host microbiota interactions, and provided a way for further study on the environmental factors of intestinal bacteria-fungi interaction.

show abstract

16 S rRNA gene diversity and gut microbial composition of the Indian white shrimp (Penaeus indicus)

Cited by 12 publications

References 57 publications

Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus

Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus

The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (Macrobrachium rosenbergii)

Exploratory study on interaction between bacteria and fungi from mulberry to intestine of Bombyx mori

Contact Info

Product

Resources

About