16-23S rRNA Spacer Region Polymorphism in Gangetic River Water Isolates of Salmonella

Abstract

Salmonella is one of the major pathogenic bacteria present in contaminated water. 16-23S rRNA spacer region has been reported to be polymorphic at serovar level in Salmonella. Salmonella isolates obtained from Ganges river water were studied for 16-23S rRNA spacer region polymorphism. Thirty three isolates belonging to eight serovars (S. Typhimurium, S. Abuja, S. Pantypridd, S. Lagos, S. Chinkual, S. Zwickau, S. Goldenberg and S. Oritamerin) were studied for the polymorphism. Out of 33 isolates, 15 different profiles were observed no serovar specific profile. Our findings indicate that 16-23S rRNA spacer region is not specific at serovar level, but can be used for differentiation of different Salmonella isolates

Nanogold based lateral flow assay for the detection of Salmonella typhi in environmental water samples

“…13 The Ganges River is considered as the lifeline of the Indian subcontinent and its microbial load is continuously increasing. Singh and Saxena (2010) 14 isolated thirty three isolates of Salmonella belonging to eight serovars from the Ganges water, India. Studies also showed that the addition of untreated sewage waste in river bodies has increased the prevalence of fecal origin Salmonella in different stretches of Ganges River, which is used majorly for drinking and domestic work.…”