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Bertollo, Luiz Antônio Carlos; Fontés, Michel; Fenocchio, Alberto Sérgio; Cano, J.

doi:10.1023/a:1018477232354

Cited by 88 publications

(13 citation statements)

References 11 publications

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“…The distribution of heterochromatin in all the karyomorphs of the Hoplias malabaricus complex has often been described in the terminal and pericentromeric region of some pairs of chromosomes ( Dergam and Bertollo 1990 , Haff et al 1993 , Bertollo et al 1997a , 1997b , Born and Bertollo 2000 , Vicari et al 2005 , Blanco et al 2010 ), and was also observed in the population of the present study. However, a small variation in the amount and location of heterochromatin can be observed between the various allopatric populations already studied (i.e., Blanco et al 2010 ).…”

Section: Discussionsupporting

confidence: 83%

Basic cytogenetics and physical mapping of 5S and 18S ribosomal genes in Hoplias malabaricus (Osteichthyes, Characiformes, Erythrinidae) from isolated natural lagoons: a conserved karyomorph along the Iguaçu river basin

Gemi¹,

Lui²,

Treco³

et al. 2014

CCG

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Erythrinidae include Neotropical teleost fish that are widely distributed in South America. Hoplias Gill, 1903 include two large groups: H. malabaricus Bloch, 1794 and H. lacerdae Miranda Ribeiro, 1908. Hoplias malabaricus is characterized by remarkable karyotype diversity, with some karyomorphs widely distributed geographically while others are more restricted to certain river basins. Cytogenetic analyzes were performed in a population of Hoplias malabaricus from the Wildlife Refuge of Campos de Palmas, the Iguaçu River basin. The specimens showed diploid number of 42 chromosomes (24m+18sm) without differentiated sex chromosomes system. The impregnation by silver nitrate showed multiple AgNORs. Seven pairs (4, 7, 10, 13, 16, 20 and 21) carrying 18S rDNA were detected by FISH. Heterochromatin was verified in the centromeric and pericentromeric region of most chromosomes and the terminal region of some pairs. FISH with 5S rDNA probes showed two chromosome pairs carrying these sites in the interstitial region (8 and 14). The data obtained in this study are similar to those found for two other populations of H. malabaricus already studied in the basin of the Iguaçu River, confirming the hypothesis that this species is natural, not having been introduced, as well as having an intrinsic characteristic, such as the largest number of sites of 18S rDNA.

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Section: Discussionsupporting

confidence: 83%

Basic cytogenetics and physical mapping of 5S and 18S ribosomal genes in Hoplias malabaricus (Osteichthyes, Characiformes, Erythrinidae) from isolated natural lagoons: a conserved karyomorph along the Iguaçu river basin

Gemi¹,

Lui²,

Treco³

et al. 2014

CCG

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“…Three well-differentiated sex chromosome systems occur in this group, namely XX/XY in karyomorph B, X 1 X 1 X 2 X 2 /X 1 X 2 Y in karyomorph D and XX/XY 1 Y 2 in karyomorph G; additionally, karyomorph C has an early differentiated XX/XY system [18,19]. Karyomorph D has 2n = 40 chromosomes in females and 2n = 39 chromosomes in males; repetitive DNA chromosomal mapping suggests that karyomorph D may have derived from a karyotype similar to karyomorph C, which is characterized by 2n = 40 chromosomes in both sexes [19,20]. A conspicuous proximal GC-rich heterochromatic/18S rDNA site, which is present on the long arms of the X and Y chromosomes in karyomorph C, is also located in the same region on the X 1 chromosome and the short arm of the large Y chromosome of karyomorph D; this suggests that the X 1 and Y chromosomes of karyomorph D derived from the XY chromosomes of karyomorph C. In addition, the Y chromosome of karyomorph D shares similar DNA sequences with chromosomes Y and 20 of karyomorph C. Chromosomal pair number 20 in karyomorph C and its homolog in karyomorph D (X 2 ) have centromeric satellite 5S Hind III-DNA, as well as an exclusive interstitial site that is present on the long arms of the Y chromosome of karyomorph D, which is the only non-centromeric location of this DNA sequence in the entire karyotype.…”

Section: Introductionmentioning

confidence: 99%

“…These data indicate that this interstitial site is derived from the centromere of chromosome 20, which was fused to the ancestral Y chromosome in karyomorph C and resulted in the dicentric Y chromosome currently present in karyomorph D [19]. Moreover, additional studies have shown that this dicentric Y chromosome behaves as a stable component of the karyotype having a correct segregation during meiosis [20]. …”

Section: Introductionmentioning

confidence: 99%

Cross-species chromosome painting tracks the independent origin of multiple sex chromosomes in two cofamiliar Erythrinidae fishes

et al. 2011

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BackgroundThe Erythrinidae fish family is characterized by a large variation with respect to diploid chromosome numbers and sex-determining systems among its species, including two multiple X1X2Y sex systems in Hoplias malabaricus and Erythrinus erythrinus. At first, the occurrence of a same sex chromosome system within a family suggests that the sex chromosomes are correlated and originated from ancestral XY chromosomes that were either homomorphic or at an early stage of differentiation. To identify the origin and evolution of these X1X2Y sex chromosomes, we performed reciprocal cross-species FISH experiments with two sex-chromosome-specific probes designed from microdissected X1 and Y chromosomes of H. malabaricus and E. erythrinus, respectively.ResultsOur results yield valuable information regarding the origin and evolution of these sex chromosome systems. Our data indicate that these sex chromosomes evolved independently in these two closed related Erythrinidae species. Different autosomes were first converted into a poorly differentiated XY sex pair in each species, and additional chromosomal rearrangements produced both X1X2Y sex systems that are currently present.ConclusionsOur data provide new insights into the origin and evolution of sex chromosomes, which increases our knowledge about fish sex chromosome evolution.

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“…The systems with male heterogamety have the first records in Neotropical fish in the genera Hoplias, with multiple sex chromosomes such as X 1 X 1 X 2 X 2 /X 1 X 2 Y and XX/XY 1 Y 2 [ 65 ], and Eigenmannia, with a X 1 X 1 X 2 X 2 /X 1 X 2 Y system [ 66 ].…”

Section: The Origin and Differentiation Of Sexual And B Chromosomementioning

confidence: 99%

Inferring Diversity and Evolution in Fish by Means of Integrative Molecular Cytogenetics

Artoni

Castro

Jacobina

et al. 2015

The Scientific World Journal

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Fish constitute a paraphyletic and profusely diversified group that has historically puzzled ichthyologists. Hard efforts are necessary to better understand this group, due to its extensive diversity. New species are often identified and it leads to questions about their phylogenetic aspects. Cytogenetics is becoming an important biodiversity-detection tool also used to measure biodiversity evolutionary aspects. Molecular cytogenetics by fluorescence in situ hybridization (FISH) allowed integrating quantitative and qualitative data from DNA sequences and their physical location in chromosomes and genomes. Although there is no intention on presenting a broader review, the current study presents some evidences on the need of integrating molecular cytogenetic data to other evolutionary biology tools to more precisely infer cryptic species detection, population structuring in marine environments, intra- and interspecific karyoevolutionary aspects of freshwater groups, evolutionary dynamics of marine fish chromosomes, and the origin and differentiation of sexual and B chromosomes. The new cytogenetic field, called cytogenomics, is spreading due to its capacity to give resolute answers to countless questions that cannot be answered by traditional methodologies. Indeed, the association between chromosomal markers and DNA sequencing as well as between biological diversity analysis methodologies and phylogenetics triggers the will to search for answers about fish evolutionary, taxonomic, and structural features.

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Cited by 88 publications

References 11 publications

Basic cytogenetics and physical mapping of 5S and 18S ribosomal genes in Hoplias malabaricus (Osteichthyes, Characiformes, Erythrinidae) from isolated natural lagoons: a conserved karyomorph along the Iguaçu river basin

Basic cytogenetics and physical mapping of 5S and 18S ribosomal genes in Hoplias malabaricus (Osteichthyes, Characiformes, Erythrinidae) from isolated natural lagoons: a conserved karyomorph along the Iguaçu river basin

Cross-species chromosome painting tracks the independent origin of multiple sex chromosomes in two cofamiliar Erythrinidae fishes

Inferring Diversity and Evolution in Fish by Means of Integrative Molecular Cytogenetics

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