149. Cryopreservation of the axial meristem of Crocus sativus L.

Zadeh, Saeid Malek; Khosrowshahli, Mahmood; Taeb, M.

doi:10.1016/j.cryobiol.2009.10.163

Cited by 4 publications

(2 citation statements)

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“…Cryopreservation offers a cost-effective and efficient preservation method for this genus. MalekZadeh et al [113] were the first to describe the successful vitrification procedure on the shoot tips of Crocus sativus. They indicated that the size of the corm is a crucial factor in explant survival after storage in LN.…”

Section: Iridaceae Familymentioning

confidence: 99%

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Merzougui

Benelli̇

Boullani

et al. 2023

Plants

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Nowadays, plant genetic resources are often at risk of loss and destruction. Geophytes are herbaceous or perennial species that are annually renewed by bulbs, rhizomes, tuberous roots, or tubers. They are often subject to overexploitation, which, combined with other biotic and abiotic stresses, can make these plants more vulnerable to a decline in their diffusion. As a result, multiple endeavors have been undertaken to establish better conservation strategies. Plant cryopreservation at ultra-low temperatures in liquid nitrogen (−196 °C) has proven to be an effective, long-term, low-cost, and suitable conservation method for many plant species. Over the last two decades, major advances in cryobiology studies have enabled successful explants of multiple genera and types, including pollen, shoot tips, dormant buds, and zygotic and somatic embryos. This review provides an update on recent advances and developments in cryopreservation and its application to medicinal and ornamental geophytes. In addition, the review includes a brief summary of factors limiting the success of bulbous germplasm conservation. The critical analysis underpinning this review will benefit biologists and cryobiologists in their further studies on the optimization of geophyte cryopreservation protocols and will support a more complete and wider application of knowledge in this area.

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Section: Iridaceae Familymentioning

confidence: 99%

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Merzougui

Benelli̇

Boullani

et al. 2023

Plants

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“…During the last two decades or so, number of media have been used by various researchers for in vitro studies in Saffron. Comparative studies have been undertaken between Murashige and Skoog (MS) and Linsmaier and Skoog (LS) media [18], LS for callogenesis and organogenesis [19], B5 for indirect and direct regeneration [20] and 1/2MS for direct regeneration of shoot in saffron [21]. In the present studies we used complete MS medium as it has been shown to be the best suited medium for complete propagation of saffron.…”

Section: Callus Production From Corm Slices In Vitro and Regenerationmentioning

confidence: 99%

Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

et al. 2020

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Background: Crocus sativus is a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties in Agrobacterium mediated transformation and vegetative reproduction. Effective genome editing requires proficient callus production and an efficient method to deliver Cas9 and sgRNAs into the plant. Here, we demonstrate Agrobacterium-mediated transformation of saffron. Further, we developed a CRISPR-Cas9 based system in this plant, for efficient gene knockout or edits in future. Results: Efficient callus production and regeneration confers important benefits in developing competent transformation system in plants. More than 70% multiplication rate of callus initiation was achieved from corm slices of saffron subjected to a two-step sterilization procedure and grown on complete MS medium supplemented with 2,4-D (0.5 mg/L), BAP (1 mg/L), IAA (1 mg/L), photoperiod of 16/8 h and 45% relative humidity at 20 ± 2 °C. In vitro cormlet generation was accomplished in 8 weeks by using mature somatic embryos on MS medium supplemented with TDZ (0.5 mg/L) + IAA (1 mg/L) + Activated charcoal (0.1 g/L) at 15 ± 2 °C. The attempt of using Agrobacterium-mediated transformation resulted in successful integration of the binary vector into the somatic embryos of saffron with a transformation efficiency of 4%. PCR and Southern blot analysis confirmed the integration of Cas9 into saffron. Conclusion: The protocol for callus production, somatic embryogenesis and regeneration was standardised. Successful demonstration of integrated Cas9 in this study constitutes first step in developing strategies for genetic manipulation of saffron, which has so far been considered recalcitrant. Furthering the development of this technology holds significant potential for advancing genetic research in saffron by integrating multigene targeting and/or use of recyclable cassettes.

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Conservation of Spices and Tree Borne Oil Seed Crops

Chaudhury

Malik

2012

Conservation of Tropical Plant Species

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149. Cryopreservation of the axial meristem of Crocus sativus L.

Cited by 4 publications

References 0 publications

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Conservation of Spices and Tree Borne Oil Seed Crops

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